Molecularly imprinted microsphere and preparation method thereof, as well as hydrogen peroxidase inhibitor
A technology of molecularly imprinted microspheres and catalase, which is applied in the directions of alkali metal oxides/hydroxides, other chemical processes, chemical instruments and methods, etc. to overcome the inefficient and complex synthesis steps
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[0028] The invention provides a method for preparing molecularly imprinted microspheres, comprising the following steps:
[0029] A) The emulsion system obtained by mixing cyclohexane, isopropanol, water, emulsifier, catalyst and precursor is sequentially subjected to heating reaction, centrifugation, washing, drying and calcination to obtain monodisperse fibrous silica microspheres;
[0030] B) mixing the monodisperse fibrous silica microspheres, 3-aminopropyltriethoxysilane and toluene for a heating reaction to obtain amino-functionalized silica microspheres;
[0031] C) Mixing the amino-functionalized silica microspheres, weakly alkaline buffer solution, catalase and dopamine, carrying out polymerization reaction and then centrifuging and washing to obtain molecularly imprinted microspheres.
[0032] In the invention, firstly, cyclohexane, isopropanol, water, emulsifier, catalyst and precursor are mixed to obtain an emulsion system.
[0033] Wherein, the emulsifier is cety...
Embodiment 1
[0057] 1) Synthesis of silica microspheres with fibrous structure (F-SiO 2 ).
[0058] First, 1.0 g of cetyltrimethylammonium bromide (CTAB) and 0.6 g of urea were dissolved in 30 mL of deionized water, and then 30 mL of cyclohexane, 1 mL of isopropanol and 1.8 mL of tetraethylorthosilicate were added in sequence. The above mixture was mechanically stirred at room temperature for 30 min (250 rpm), and then heated to 75° C. for 16 h. Pour the reaction solution into a centrifuge tube, centrifuge at a speed of 8000rpm for 10min (Flying Pigeon TGL-16C centrifuge), pour the upper layer filtrate into the waste liquid bucket, soak and wash the product sinking at the bottom of the centrifuge tube with absolute ethanol, and then Centrifugation under the same conditions was performed. After repeating the ethanol washing-centrifugation steps above twice, the product was collected in a glass petri dish and dried in a Boxun GZX-9070MBE blast oven at 50°C to obtain a white powder. The ab...
Embodiment 2
[0064] 1) Synthesis of silica microspheres with fibrous structure (F-SiO 2 ).
[0065] First, 1.0 g of cetyltrimethylammonium bromide (CTAB) and 0.6 g of urea were dissolved in 30 mL of deionized water, and then 30 mL of cyclohexane, 1 mL of isopropanol and 2.5 mL of tetraethylorthosilicate were added in sequence. The above mixture was mechanically stirred at room temperature for 30 min (250 rpm), and then heated to 75° C. for 16 h. Pour the reaction solution into a centrifuge tube, centrifuge at a speed of 8000rpm for 10min (Flying Pigeon TGL-16C centrifuge), pour the upper layer filtrate into the waste liquid bucket, soak and wash the product sinking at the bottom of the centrifuge tube with absolute ethanol, and then Centrifugation under the same conditions was performed. After repeating the ethanol washing-centrifugation steps above twice, the product was collected in a glass petri dish and dried in a Boxun GZX-9070MBE blast oven at 50°C to obtain a white powder. The ab...
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