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Method for extracting and separating glutathione from GSCu precipitate

A technology of glutathione and crystals, applied in the field of extraction and separation of glutathione, to achieve the effects of reducing environmental pollution, stable product quality, and improving convenience

Active Publication Date: 2016-12-14
SHANGHAI QINGPING PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the defects and deficiencies in the prior art, in order to obtain a method for separating and purifying glutathione suitable for industrial production, the inventor intends to propose a copper salt extraction and separation method using metal sulfide instead of hydrogen sulfide, aiming at Simplify production operations and effectively solve environmental pollution problems, thereby facilitating industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Add 320g of GSCu precipitate and 700ml of water into the reaction kettle together to form a suspension, stir well, then add 22ml of 36% concentrated hydrochloric acid for acidification, then add 32ml of 20% ammonium sulfide solution, and stir at 20-25°C for 1.5 hour, centrifuge to remove the generated Cu 2 Precipitate S to obtain glutathione mother liquor; concentrate said glutathione mother liquor under the pressure of -0.05Mpa and under reduced pressure at 50°C to obtain glutathione concentrate; Add 50% ethanol solution preheated to 60°C to the concentrated solution, then use hydrochloric acid to adjust the pH of the glutathione ethanol solution to 2~4, stir slowly, fully crystallize, filter with suction, and use 65% ethanol solution Wash 2 to 4 times to obtain crude glutathione crystals; vacuum-dry the crude glutathione crystals at 60°C under a pressure of -0.05 Mpa to finally obtain 46g of pure glutathione crystals; Phase (HPLC) detection, the purity of glutathione...

Embodiment 2

[0041]Add 400g of GSCu precipitate and 1200ml of water into the reaction kettle together to form a suspension, stir well, then add 60ml of 18% hydrochloric acid for acidification, then add 14g of sodium sulfide, stir and react at 20-25°C for 2 hours, and remove by centrifugation Generated Cu 2 Precipitate S to obtain glutathione mother liquor; concentrate said glutathione mother liquor under 0Mpa pressure and under reduced pressure at 60°C to obtain glutathione concentrate; add glutathione concentrate to said glutathione concentrate Add 55% ethanol solution preheated to 65°C, then use hydrochloric acid to adjust the pH of glutathione ethanol solution to 2~4, stir slowly, fully crystallize, filter with suction, and wash with 70% ethanol solution for 2 ~4 times, the glutathione crystal crude product was obtained; under the pressure of -0.04 Mpa, the glutathione crystal crude product was vacuum-dried at 60°C to finally obtain 60g glutathione crystal pure product; through high per...

Embodiment 3

[0044] Add 380g of GSCu precipitate and 1100ml of water into the reaction kettle together to form a suspension, stir well, then add 10ml of 98% concentrated sulfuric acid for acidification, then add 19g of potassium sulfide, stir and react at 25-30°C for 2 hours, centrifuge Remove the generated Cu 2 Precipitate S to obtain glutathione mother liquor; concentrate said glutathione mother liquor at -0.06Mpa pressure and under reduced pressure at 50°C to obtain glutathione concentrate; add glutathione to said glutathione Add 50% ethanol solution preheated to 55°C to the concentrated solution, then use hydrochloric acid to adjust the pH of glutathione ethanol solution to 2~4, stir slowly, fully crystallize, filter with suction, and use 65% ethanol solution Wash 2 to 4 times to obtain crude glutathione crystals; vacuum-dry the crude glutathione crystals at 55°C under a pressure of -0.05 Mpa to finally obtain 53g of pure glutathione crystals; Phase (HPLC) detection, the purity of glu...

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Abstract

The invention provides a method for extracting and separating glutathione from GSCu precipitate. The method comprises the following steps: adding GSCu precipitate and water into a reactor, forming suspension liquid, completely stirring, adding acid to perform acidification, adding sulfide, reacting under the stirring condition, centrifuging or filtering, and removing the generated Cu2S precipitate to prepare a glutathione mother liquid; concentrating and crystallizing to prepare a glutathione crystal pure product, wherein the sulfide is selected from one or more of the following compounds: ammonium sulfide, sodium sulfide and potassium sulfide. By the method, the raw materials are effectively selected, and the proportion and the reaction condition are reasonably controlled and the use of hydrogen sulfide is avoided, so that environmental pollution is reduced, the convenience of production and operation is improved, the prepared glutathione has stable product quality, and the method is quite suitable for large-scale industrialized production. In conclusion, the method for extracting and separating glutathione from the GSCu precipitate, provided by the invention, has good application prospect and market potential.

Description

technical field [0001] The invention belongs to the field of biochemical industry and relates to a method for separating and purifying glutathione, in particular to a method for extracting and separating glutathione from GSCu precipitation. Background technique [0002] Glutathione (Glutathione, GSH), full name γ-L-glutamic acid-L-cysteinyl glycine, is a sulfhydryl-containing compound formed by condensation of L-glutamic acid, L-cysteine ​​and glycine. Biologically active tripeptide is one of the important non-protein sulfhydryl compounds in living organisms. [0003] GSH has multiple functions in the living body, participates in various metabolisms in the body, and especially plays an important role in maintaining the redox balance in the body. Based on its strong electron donor ability, GSH can protect biomolecules such as DNA and protein from oxidative damage such as free radicals. GSH has a detoxification effect, and can combine with heavy metal ions, carbon monoxide, ...

Claims

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Application Information

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IPC IPC(8): C07K5/037C07K1/30C07K1/14
CPCC07K5/0215
Inventor 刘世领汤晶
Owner SHANGHAI QINGPING PHARMA CO LTD
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