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Calorimetric bionic detection method for detecting pesticide Atrazine residue

A technology of atrazine and detection method, which is applied in the field of biosensors, can solve the problems of lack of specificity, etc., and achieve the effect of simple pretreatment, good consistency and sensitive detection method

Inactive Publication Date: 2017-01-11
INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the total exothermic heat of the thermal signal reaction, the lack of specificity of detection is the deficiency of this type of detection method

Method used

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  • Calorimetric bionic detection method for detecting pesticide Atrazine residue
  • Calorimetric bionic detection method for detecting pesticide Atrazine residue
  • Calorimetric bionic detection method for detecting pesticide Atrazine residue

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Experimental program
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Effect test

preparation example Construction

[0021] 1. Preparation of s-MIP

[0022] (1) Dissolve 2.85g of atrazine in 100ml of N, N-dimethylformamide (DMF) acetate buffer mixed solution (DMF: acetate buffer = 9: 1, v / v; acetate buffer, 0.2 mol / L, pH3.8). Make it dissolve completely under the condition of mechanical stirring;

[0023] (2) Add 13.63g p-sodium styrene sulfonate (SSS), 1.46g methylenebisacrylamide (BIS) and 400mg controllable hollow glass beads (CPG) into the mixed solution of step (1) successively, Form an imprinted pre-polymerization system;

[0024] (3) Pre-polymerize the system for 12 hours in a water bath at 38°C with a stirrer rotating at 300 rpm;

[0025] (4) The pre-polymerization system was fed with nitrogen for 30 minutes, and ammonium persulfate (APS) was added to initiate graft imprinting polymerization, and the reaction was carried out for 10 hours;

[0026] (5) The imprinted product is separated from the system by vacuum filtration through a sand core funnel;

[0027] (6) Regenerate the p...

Embodiment 1

[0048] (1) Put the surface imprinted product (prepared according to the method in step 1) into the reactor and place it in a heat regulator, and pass through the carrier liquid (0.02mol L-1, pH3. 8 acetic acid-sodium acetate buffer solution, containing 2% DMF) equilibrium flow system, the flow rate of the system is 800 μl min-1;

[0049] (2) Use carrier liquid as solvent to prepare atrazine solution with gradient concentration of 0-120 μg mL-1.

[0050] (3) The sample solution is added, and the thermal signal changes accompanying the binding and dissociation process of the analyte to the imprinted hole are collected by the Wheatstone bridge, converted and displayed as peak signals on the calorimetric workstation (N2000 chromatography data workstation); a single sample The detection takes about 5 minutes and can be continuously detected without regeneration.

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Abstract

The invention provides a calorimetric bionic detection of typical pesticide Atrazine residue by combining molecular imprinting technology and calorimetric sensing detection technology. The detection has the advantages of simple pre-treatment of samples without labeling, fast 'one step method' and specificity, and the disadvantage of complex pre-treatment of samples for detection by large-scale instruments is overcome. The detection sensitivity is ppm level, linearity range of the method is 3.9-120 [mu]g / mL, and detection limit is 1.1[mu]g / mL. Standard addition recovery experiments and methodology comparison results demonstrate that the detection and HPLC method detection of Atrazine have good consistency. The rapid, reliable and sensitive detection method for quantitative determination of typical pesticide Atrazine residue is provided.

Description

technical field [0001] The invention relates to a calorimetric bionic detection method for detecting pesticide atrazine residues, which belongs to the field of biosensors and is used for fast, sensitive and accurate detection of pesticide atrazine residues in food or water source samples, and has advantages that large-scale instruments cannot match . Background technique [0002] Herbicide Atrazine (Atrazine, ATR) is also known as atrazine, its full name is 2-chloro-4-ethylamine-6-isopropylamine-1,3,5-triazine, and its molecular weight is 215.69 Triazine herbicides As a selective systemic conduction pre-emergence and post-emergence herbicide, it achieves the purpose of removing weeds by inhibiting photosynthesis. It has been used in a large area since its development and production in the 1950s, mainly for corn, sorghum, sugarcane, and fruit trees. Dry fields such as woodlands, woodlands, etc., among which atrazine (ATR) is the most widely used; ATR has a certain residual t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N25/20G01N1/28
Inventor 高志贤郄志伟宁保安孙思明彭媛白家磊
Owner INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL