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Technological method for producing sodium gluconate by enzyme method

A technology of sodium gluconate and glucose, applied in the field of biochemical engineering, can solve problems such as shortage, and achieve the effects of improving production rate, reducing production cost, and saving the amount of enzyme added.

Inactive Publication Date: 2017-01-18
山东福洋生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In summary, there is a lack of an optimal method for the enzymatic production of sodium gluconate in this area, and there is an urgent need for a method that saves the amount of enzyme added, reduces production costs, and improves the production rate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] In the first step, add 30L of reaction liquid in a 50L tank, in which the glucose content is 300g / L, and add 6ml of defoamer;

[0026] In the second step, add 31.5ml of catalase (the addition amount is 3.5kg / T dry matter) and 10ml of glucose oxidase (the enzyme activity in the tank is greater than 2500U / ml) to the tank to start the reaction, and add 35% sodium hydroxide Control PH5.5-6.0, control speed and air volume to maintain 30%-40% dissolved oxygen in the tank.

[0027] The third step is to add glucose oxidase to detect the content of glucose oxidase in the reaction solution. When the enzyme activity drops to 2100U / ml, start to add glucose oxidase (873,600 U / ml) to make the glucose oxidase in the reaction solution The enzyme activity was maintained at 2000U / ml. End the reaction when the glucose content is lower than 3g / L. The reaction time is 20 hours, and the total amount of glucose oxidase used is 28ml (equivalent to an addition amount of 3.1kg / T dry matter). ...

Embodiment 2

[0033] In the first step, add 30L of reaction liquid in a 50L tank, in which the glucose content is 350g / L, and add 6ml of defoamer;

[0034] In the second step, add catalase 36.75m1 (375,000 U / m1) and glucose oxidase 10ml (enzyme activity in the tank is greater than 2500U / m1) to the tank to start the reaction, and add 35% sodium hydroxide to control the pH5. 5-6.0, control the speed and air volume to maintain the dissolved oxygen in the tank at 30%-40%.

[0035] The third step is to add glucose oxidase to detect the content of glucose oxidase in the reaction solution. When the enzyme activity drops to 2600U / ml, start to add glucose oxidase (873,600 U / ml) to make the glucose oxidase in the reaction solution The enzyme activity is maintained at 2500U / ml. End the reaction when the glucose content is lower than 3g / L. The reaction time is 18.5 hours, and the total amount of glucose oxidase used is 31ml (the added amount is 2.95kg / T dry matter).

[0036] The fourth step, enzyme ...

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Abstract

The invention provides an efficient enzyme method for producing sodium gluconate, comprising the following steps: carrying out an enzymatic reaction by adding catalase and glucose oxidase into a reaction tank containing glucose as a raw material; adding glucose oxidase to maintain enzyme activity of glucose oxidase in the reaction solution at 2000U / ml-2500U / ml, and stopping the reaction when content of glucose is lower than 3g / L; and crystallizing, separating and drying obtain a sodium gluconate finished product. In comparison with enzyme addition amount in the prior art, enzyme addition amount in the method at least can be reduced by 10%.

Description

technical field [0001] The invention relates to the field of biochemical engineering, in particular to a process for enzymatically producing sodium gluconate. Background technique [0002] Sodium gluconate, also known as D-sodium gluconate, is widely used in industry. It can be used in food, medicine, chemical industry, water treatment and other industries. It can be used as high-efficiency retarder and high-efficiency water reducer in the concrete industry. [0003] At present, the production methods of sodium gluconate mainly include microbial fermentation method, electrolysis method, catalytic oxidation method, double enzyme method and other methods. Due to the mild conditions of the microbial method and obvious energy saving, it is widely used. The fermentation of gluconic acid and its salts (such as sodium gluconate, calcium gluconate, etc.) is mainly carried out by mold fermentation. The process is to add a large amount of medium to the fermenter and then insert the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/58
CPCC12P7/58
Inventor 赵伟曹大鹏方建任峰马春燕高文学
Owner 山东福洋生物科技股份有限公司
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