A kind of green preparation method of porous graphene

A porous graphene and graphene technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of limited application fields, high cost, and inability to be widely promoted, and achieve a green and pollution-free process. Mild conditions, convenient and easy to obtain the effect of fecundity

Active Publication Date: 2020-01-17
HEFEI GUOXUAN HIGH TECH POWER ENERGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned traditional porous graphene preparation method is complicated in process and high in cost, and cannot be widely promoted, which limits its application field

Method used

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  • A kind of green preparation method of porous graphene
  • A kind of green preparation method of porous graphene

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preparation example Construction

[0027] Preparation of seed culture solution: Boil 200g of fresh potato pieces with water for 30 minutes, mash them and filter with 8 layers of gauze to obtain the filtrate; then add 20g of glucose, 1.5g of potassium dihydrogen phosphate, and 0.75g of magnesium sulfate for mixing, and finally use deionized water Dilute to 1 L, sterilize at 121°C for 20 min, and cool to room temperature.

[0028] Preparation of liquid nutrient-limited medium: Glucose 10g, ammonium tartrate 0.1g, potassium dihydrogen phosphate 2g, calcium chloride 0.1g, magnesium sulfate 0.25g, ferrous sulfate 0.1g, veratrol 150mg, sodium chloride 1mg, VB with Tween-80 5 mg, manganese sulfate 0.5 mg 1 Mix 5 mg with deionized water to 1 L and adjust the pH to 5.0; finally sterilize at 121°C and cool to room temperature.

Embodiment 1

[0030] A kind of green preparation method of porous graphene of the present invention comprises the following steps:

[0031] (1) Immobilized white rot fungi

[0032] Put 1 L of seed culture solution into 4 Erlenmeyer flasks, add 4 pieces of bacterial cellulose membrane (1.5 cm×1.5 cm×0.5 cm) into each Erlenmeyer flask, sterilize and cool;

[0033] Then inoculate 1×10 in each Erlenmeyer flask 7 CFU of spore suspension of white-rot fungus 5 mL, in which the spore concentration is 2 × 10 5 CFU; then place the flask in a constant temperature shaking incubator at 150 r / min and 30°C for 5 days, so that the hyphae can attach to and completely wrap the bacterial cellulose membrane; finally take it out and wash the surface with sterile water Three times, remove the residual culture medium to obtain immobilized white rot fungi;

[0034] (2) Preparation of porous graphene by biodegradation

[0035] Divide 1 L of liquid nutrient-limited medium into four 1 L Erlenmeyer flasks (250 mL / ...

Embodiment 2

[0040] A kind of green preparation method of porous graphene of the present invention comprises the following steps:

[0041] (1) First divide the seed culture solution into 4 Erlenmeyer flasks, add 2 bacterial cellulose membrane blocks (2.5 cm×2.5 cm×1.5 cm) into each Erlenmeyer flask, sterilize and cool;

[0042] Inoculate 2×10 7 5 mL of spore suspension of CFU white-rot fungi, in which the spore concentration is 4 × 10 5CFU; place the above four Erlenmeyer flasks in a constant temperature shaking incubator at 180 r / min, 30°C and incubate for 4 days, mycelium adheres to and completely wraps the bacterial cellulose membrane block, takes it out and cleans the surface three times with sterile water to remove The remaining culture medium is the immobilized white-rot fungus;

[0043] (2) Divide 1 L of liquid nutrient-limited medium into four 1 L Erlenmeyer flasks (250 mL / bottle), then add 2 pieces of immobilized white-rot fungi to each Erlenmeyer flask, and place in a constant ...

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Abstract

The invention discloses an environment-friendly preparation method of porous graphene. The environment-friendly preparation method comprises the following steps: inoculating spore suspension liquid of white-rot fungi into a seed culture solution containing bacterial cellulose membrane blocks, and carrying out culturing, so as to obtain fixed white-rot fungi membrane blocks; inoculating the fixed white-rot fungi membrane blocks into a liquid nutrition-limited culture medium, carrying out culturing for 1-3 days, and adding oxidized graphene suspension liquid to react for 5-24 hours until oxidized graphene adsorbs the surfaces of hyphae of the fixed white-rot fungi membrane blocks; carrying out biological oxidation and degradation, so as to obtain degraded oxidized graphene membrane blocks; peeling hyphae externally adsorbed with oxidized graphene by virtue of tweezers, adding the peeled hyphae into diluted hydrochloric acid, and heating to dissolve; carrying out centrifuging and washing, so as to obtain purified porous graphene suspension liquid; and finally, drying, so as to obtain porous graphene. According to the preparation method, the porous graphene is prepared from fixed white-rot fungi under mild conditions, so that the reaction process is environmentally friendly, the cost is low, and the hole distribution of porous graphene is relatively wide.

Description

technical field [0001] The invention relates to a preparation method of graphene materials, in particular to a green preparation method of porous graphene. Background technique [0002] In 2004, graphene was successfully prepared by Professor Andre K. Geim of the University of Manchester, UK, using a simple and easy tape stripping (mechanical stripping) method. Due to its excellent electrical properties, thermal properties, mechanical properties, optical properties and mechanical properties, it is expected to have great application prospects in microelectronics, energy, information, biomedicine, especially in the field of nanomaterials. At present, the preparation methods of graphene mainly include the "top-down" preparation method using graphite as the raw material and the "bottom-up" preparation method using small molecular carbon as the precursor. Among the "top-down" preparation methods The liquid-phase ultrasonic dispersion method, the chemical oxidation-reduction meth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P3/00C12R1/645
CPCC12P3/00
Inventor 朱春林杨尘杨茂萍曹勇
Owner HEFEI GUOXUAN HIGH TECH POWER ENERGY
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