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Culture medium and tremella fuciformis strain prepared by using same

A technology for cultivating substrates and white fungus, applied in the field of white fungus strains, can solve the problems of no growth of white fungus hyphae, betrayal, reduced activity of cultivated species, etc., and achieve the effects of improving biotransformation rate, uniform distribution of hyphae, and shortening germination time.

Inactive Publication Date: 2017-02-01
SICHUAN DAZHEN SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The growth range of Tremella fungus mycelia can only extend to about 2cm from the middle point, and the growth range of the mycelia of its associated bacteria (Ash fungus) can spread all over the whole fungus bag, and usually the diameter of the fungus bag is not less than 8cm after stretching ; This has just caused the hyphae of the associated bacteria to grow only on the side away from the center in the fungus bag, but no white fungus hyphae
The inoculation of white fungus is usually carried out in two ways, one is to inoculate by breaking blocks, and the growers often cannot distinguish between white fungus mycelia and incense ash mycelia, so that there are only incense ash mycelia and no white fungus mycelium in the inoculation hole, and holes appear Phenomenon; one is to plant the cultivars through a funnel-shaped tool after completely kneading the cultivars. This method can easily cause mycelium damage and reduce the activity of the cultivars. Hyphae phenomenon
[0006] It can be seen that the ear yield rate will be much lower than expected by using the currently commercially available cultivars, which not only wastes production materials, but also disappoints the efforts of the growers, reducing the production efficiency of white fungus
Even if growers can understand the biological characteristics of white fungus, can distinguish whether the cultivated species contain white fungus mycelia, and discard the part that only contains ash fungus during the cultivation process, this also causes a waste of resources and increases costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～3

[0040] Prepare the culture substrate with the formula in Table 1:

[0041] Table 1 culture substrate formula

[0042] sawdust bran cottonseed hulls rice bran plaster protein powder Potassium dihydrogen phosphate magnesium sulfate Example 1 60kg 23kg 10kg 5kg 1kg 1kg 0.2kg 0.05kg Example 2 50kg 20kg 15kg 7kg 0.5kg 2kg 0.19kg 0.047kg Example 3 70kg 30kg 5kg 3kg 2kg 0.5kg 0.22kg 0.055kg

[0043] The Qinggang sawdust is crushed and passed through a 10-mesh sieve. According to the recipe, mix greenwood sawdust, bran, cottonseed hulls, rice bran, gypsum, insect protein powder, potassium dihydrogen phosphate and magnesium sulfate, and sterilize at 123°C for 150 minutes to prepare a culture substrate.

[0044] The culture substrate is made into a truncated cone with a height of 30mm; the diameters of the two bottom surfaces are 18mm and 16mm respectively. After the sterilization of the culture...

Embodiment 4

[0057] Embodiment 4 Indoor test ear output rate

[0058] The main purpose is to truncate the Qinggang section wood to a more suitable size, and through high-pressure sterilization, it not only retains the active ingredients of the Qinggang section wood, and simulates natural growth; it also shortens the growth cycle of the section wood for earing, which is helpful for verifying new varieties and new processes. The development effect is fast and effective. Take the clinker soaked in lime water with a height of 10cm, the short section drilled with an electric drill, and autoclaved, that is, the short section clinker as the test carrier for the ear yield rate of Tremella.

[0059] The cultivars prepared in Examples 1-3 were used as experimental objects, and they were inserted into the inoculation holes of short sections of clinker to observe and record the rate of ear production and indoor testing of tremella traits.

[0060] The cultivars prepared in Comparative Examples 1-3 we...

Embodiment 5

[0066] Embodiment 5 Outdoor test ear output rate

[0067] The outdoor test of the fruiting rate was carried out with the wood cut in Chenhe Township, the main producing area of ​​Tremella tremella in Tongjiang section.

[0068] Taking the cultivars of Examples 1-3 and the cultivars of Comparative Examples 1-3 as the experimental objects, in Chenhe Township according to the local farming arrangements, after the Spring Festival, cut down the Qinggang tree, dry it for about 20 days, and cut it into 1 meter when the solar term of Awakening Insects uniform segment of wood. After being transported back to the place of production, the holes are drilled 2-3 deep, and after inoculation 40-60 days, the bacteria will develop. According to the climate conditions of the year, enter the pre-prepared ear-out hall at the end of May and early June for ear-out management; according to Tremella Spray water and insects in good time when growing. In the middle and late September, count the ears....

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Abstract

The invention relates to the technical field of edible mushroom cultivation and especially relates to a culture medium and a tremella fuciformis strain prepared by using the same. The culture medium includes wood chips, bran, cottonseed hull, rice bran, gypsum, protein powder, monopotassium phosphate, and magnesium sulfate. In the tremella fuciformis strain prepared on the culture medium, distribution of mycelia in the tremella fuciformis is uniform and a proper ratio of the mycelia to companion fungus is achieved. With the mycelia as a cultivar species in production, after inoculation, fungus growth rate is significantly increased (p < 0.05), cavity in a fungus wood rod is reduced, bio-conversion rate of the fungus wood rod is increased and incoming of farmers is increased.

Description

technical field [0001] The invention relates to the technical field of edible fungus cultivation, in particular to a culture medium and tremella fungus species prepared therefrom. Background technique [0002] Tremella fuciformis belongs to Eumycota, Basidiomycetes, Tremellales, Termelaceae, Tremell in classification. It is a mesophilic and aerobic fungus, mainly distributed in Subtropical, also distributed in tropical, temperate and frigid regions. Tremella is different from most cultivated edible fungi, it is a typical representative of associated fungi to complete its life history synergistically, and the completion of its life history is inseparable from the synergistic effect of the fungi. [0003] my country is a big producer of white fungus. In recent years, in response to market demand, the planting area of ​​white fungus has been greatly increased. In order to improve production efficiency, the quality of white fungus cultivars has received more and more attention....

Claims

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Application Information

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IPC IPC(8): C12N1/14A01G1/04C12R1/645
CPCC12N1/14
Inventor 肖奎顾世
Owner SICHUAN DAZHEN SCI & TECH CO LTD
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