Quarantine fruit fly multi-target rapid identification method

Abstract

The invention relates to a fruit fly quarantine technology, in particular to a quarantine fruit fly multi-target rapid identification method. The quarantine fruit fly multi-target rapid identification method comprises the following steps: extracting genome DNA of a to-be-detected sample; performing PCR by taking the genome DNA as a template and by using specific primers as shown in SEQ ID NO.1 and SEQ ID NO.2; and detecting the PCR product by gel electrophoresis, wherein the sample belongs to quarantine fruit flies if a 603bp specific band occurs, and the sample does not belong to the quarantine fruit flies if the 603bp specific band does not occur. The quarantine fruit flies relate to anastrepha schiner, bactrocera macquart, carpomya, ceratitis, dacus fabricius and/or rhagoletis; and the specific primers can distinguish the quarantine fruit flies from other tephritidae and diptera close species, have high universality and provide powerful technological support for effectively preventing and controlling introduction and diffusion of the quarantine fruit flies and protecting the ecological safety in China.

Description

technical field [0001] The invention relates to fruit fly quarantine technology, in particular to a multi-target rapid identification method for fruit fly quarantine. Background technique [0002] Fruit flies, belonging to Tephritidae insects of Diptera Tephritidae, are widely distributed all over the world and mostly occur in tropical and subtropical regions. There are about 500 genera and 4,500 species known in the world, of which about 1,500 are related to various fruits, and there are more than 250 species of fruit flies with economic significance. Fruit flies have a wide range of hosts. Adults lay eggs on the fruit surface of fruits and vegetables to form holes, and larvae eat inside the fruit to cause damage, resulting in fruit rot or fruit drop. The damage can cause up to 100% economic loss, which not only affects the production of fruits and vegetables. Serious harm, and have a serious impact on import and export trade. [0003] There are mainly five genera of frui...

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Problems solved by technology

[0005] In the past ten years, a variety of molecular biology techniques have been applied to the rapid identification of fruit flies, such as: DNA barcoding (DNA Barcoding), PCR technology, restriction fragment length polymorphism (Restriction Fragment Length Polymorphism, RFLP), random amplification Polymorphic DNA technology (Random Amplified Polymorphic DNA, RAPD), amplified fragment length polymorphism technology (Amplified Fragment Length Polymorphism, AFLP) and gene chip technology, but from the current reports, all studies are only for A rapid detection method has been established for individual fruit fly species, but it still cannot fully meet the detection requirements in the list of imported plant quarantine pests in the unit of high-level taxa of fruit flies

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