Mediastinal malignant teratoma detecction colloidal gold immunochromatographic test paper and preparation method thereof

Abstract

The invention provides a mediastinal malignant teratoma detecction colloidal gold immunochromatographic test paper. The test paper is prepared by the steps of: (1) preparing a colloidal gold-AFP and colloidal gold-LDH antibody complex; (2) preparing double-antibody sandwich test paper: a, preparing a combination pad: taking a glass fiber membrane as the material, and cutting it into alternative specifications for standby use; b, preparing a sample absorption pad: soaking the sample absorption pad in a PBS buffer solution for 12h, and conducting drying for standby use; c, preparing a nitrocellulose membrane: drawing a detection line 1, a detection line 2 and a quality control line at different positions on the nitrocellulose membrane in order with a membrane dispenser for standby use, wherein the detection line 1 is coated with the colloidal gold-AFP antibody complex, the detection line 2 is coated with the colloidal gold-LDH antibody complex, and the quality control line is coated with a goat-anti-rabbit monoclonal antibody; and d, assembly test paper: sticking the sample absorption pad, the combination pad, the nitrocellulose membrane and a water absorption pad on a PVC substrate in order, performing drying treatment at 40DEG C for 40min, and then cutting the product into 2cm wide test strip.

Description

technical field [0001] The invention relates to the technical field of cardiothoracic disease detection, in particular to a colloidal gold immunochromatographic test paper for detecting mediastinal malignant teratoma. Background technique [0002] A teratoma is a true tumor. There are several different types of tissue consisting of 2 or 3 germ layers, which may be composed of mature, immature, or mixed elements. Occasionally, it can be seen that a germ layer tissue component is dominant, or a highly specific tissue type is dominant. Teratoma most commonly occurs in the ovary, but it can also be found in the testis, retroperitoneum, sacrum, mediastinum, etc., and other parts are rare. The ratio of benign and malignant teratomas is related to the site of occurrence. Malignancy accounts for only 1% to 3% of ovarian cyst teratomas, and 95% of testis. In addition to the gonads, the mediastinum is also a predilection site, most of which are located in the anterior mediastinum, ...

AI Technical Summary

Problems solved by technology

[0003] At present, benign teratomas can be treated by surgical resection, but most patients with malignant teratomas have metastases that cannot be removed when they see a doctor. Even if the resection is mainly for biopsy to confirm the diagnosis, most patients relapse or metastasize and die within half a year

Clinically, patients in stage II-III generally have invasion and metastasis of surrounding tissues, and non-surgical treatment is the main treatment. At present, radiotherapy and chemotherapy are often used, but the curative effect is not good.