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Thermoactinomyces thalpoillus strain and application thereof

A high-temperature actinomycetes and mesophilic technology, applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of no articles or patents, and achieve the goal of not destroying the ecological balance, not polluting the environment, and having strong tolerance Effect

Active Publication Date: 2017-02-22
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few researches on the discovery and functional exploration of high-temperature actinomycetes in Maotai-flavored high-temperature Daqu, and there are no related articles or patents

Method used

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Examples

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Embodiment

[0033] Mesophilic thermoactinomycetes of the present invention ( Thermoactinomyces thalpohillus )Separation method:

[0034] Weigh 10g of the preserved koji powder sample from a distillery in Guizhou, mix it in a sterile beaker according to the mass ratio of the sample to CaCO3 of 10:1, add 2ml of sterile water, and enrich at 28°C with an air humidity of 30%. Cultivate for 1 week; put the enriched Daqu sample into a 90ml sterile CMC aqueous solution (2.5g / L) in a Erlenmeyer flask with glass beads, shake at 50°C and 200r / min for 30min; put the Daqu sample suspension in Heat treatment in a water bath at 50°C for 10 minutes, take 1ml of the sample suspension for 10-fold gradient dilution, respectively take 0.2ml of the sample suspension with a dilution of 10-4, 10-5, 10-6 and 10-7 and apply it on the separation On the medium, culture statically at 50°C for 3 days, pick a single colony for further screening, and store it on the slant medium.

[0035] The colonies formed by the ...

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PUM

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Abstract

The invention discloses a thermoactinomyces thalpoillus strain and application thereof. The thermoactinomyces thalpoillus strain is morphologically characterized in that the strain is cultured on an ISP2 culture medium for 3 days at 50 DEG C, wherein round protruding non-transparent colonies 6-7mm in diameter are dry, dense and less prone to being poked, have rugae and white spores and are free from soluble pigment, medium mycelia are faint yellow, and aerial mycelia are white; under observation of a scanning electron microscope, single spores are formed on the mycelia and insert on the same without stems or directly, and single side of every spore grows on two sides of the corresponding mycelium; the thermoactinomyces thalpoillus strain is waxy in texture, non-transparent and smooth in colony edge. The thermoactinomyces thalpoillus strain is capable of yielding geosmin substances.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a mesophilic high-temperature actinomycete, and also relates to the use of the mesophilic high-temperature actinomycete in producing geosmin substances. Background technique [0002] Daqu is a kind of saccharifying agent and leavening agent used in wine making. The production of Daqu has four characteristics: raw material production, open production, low degree of mechanization and simple operation. The total amount of microorganisms in the cultivation process; its cultivation has the characteristics of natural cultivation, accumulation and heating, strong seasonality, easy cultivation, long cycle, and coexistence of bacteria and enzymes. [0003] At present, the applicant has used the second-generation high-throughput sequencing method to analyze the microbial diversity of Maotai-flavored Daqu produced by liquor enterprises in Guizhou Province. Using a culture-free method...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04C12R1/01
CPCC12P1/04C12N1/205C12R2001/01
Inventor 王晓丹邱树毅罗小叶
Owner GUIZHOU UNIV
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