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Detection kit for melamine in food

A detection kit and melamine technology, which is applied in biological testing, material inspection products, and analysis through chemical reactions of materials, can solve the problems of insufficient sensitivity and reaction efficiency, so as to expand the effective reaction area, ensure stability, The effect of short reaction times

Inactive Publication Date: 2017-02-22
WUXI X RES PROD DESIGN & RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods can realize the accurate detection of melamine, their sensitivity and reaction efficiency have certain deficiencies.

Method used

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Embodiment 1

[0054] In an embodiment of the present invention, a detection kit for melamine in food includes: 1) melamine calibrator; 2) melamine-carboxyl magnetic bead linker reagent; 3) enzyme conjugate; 4) chemiluminescent substrate; 5) Cleaning fluid. The concentration gradient of melamine standard substance is 0ng / ml, 10ng / ml, 20ng / ml, 40ng / ml, 80ng / ml, 200ng / ml. The main component of the melamine-carboxyl magnetic bead connector reagent is the melamine-coupled carboxyl magnetic bead connector. The particle size of carboxyl magnetic beads is 2-3 μm, and the surface active group is carboxyl (-COOH). The main component of the enzyme conjugate is melamine-binding protein labeled with alkaline phosphatase. The main component of the chemiluminescent substrate is (3-(2-spiroadamantane)-4-methoxy-4-(3-phosphoryloxy)-phenyl-1,2-dioxetane disodium salt ).

[0055] The preparation method of the detection kit of melamine in described food, comprises the following steps:

[0056] 1) Preparat...

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Abstract

The invention discloses a detection kit for melamine in food, which comprises: 1) a melamine calibrator; 2) a melamine-carboxylate magnetic bead linker reagent; 3) an enzyme conjugate; 4) a chemiluminescent substrate; 5) cleaning solution. Through a method for directly and stably connecting melamine with the solid phase carrier, the detection kit ensures the stability in connection with the solid phase carrier, and the full exposure of the melamine molecules, and further enlarges the effective reaction area by introducing the magnetic particles, the melamine chemiluminescence immunoassay kit that is higher in sensitivity and shorter in the reaction time is provided. The kit can be applied to a variety of luminescence detectors.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a detection kit for melamine in food. Background technique [0002] Melamine, commonly known as melamine and protein essence, is a triazine nitrogen-containing heterocyclic organic compound, which is a pure white monoclinic prism. Melamine is an organic chemical raw material with a wide range of uses. Its main use is as a raw material for the production of melamine-formaldehyde resin, and it can also be used as a flame retardant, water reducer, and formaldehyde cleaner. [0003] In the food industry, the Kjeldahl method is usually used to calculate the protein content by measuring the nitrogen content. The nitrogen content of melamine is 66%, which is often illegally added to food by criminals to create the illusion that the protein content meets the standard. [0004] However, this melamine-added food not only does not really increase the protein content, but its toxic a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N33/68
CPCG01N21/76G01N33/68
Inventor 吴敏芳徐静赵春城胡勇蒋韦艳刘金杰朱倩倩
Owner WUXI X RES PROD DESIGN & RES
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