Method for preparing 2-carbonyl ethyl butyrate salicylhydrazone nickel complex and application of 2-carbonyl ethyl butyrate salicylhydrazone nickel complex
A technology of ethyl carbonyl butyrate salicyloyl hydrazone and ethyl carbonyl butyrate salicyloyl hydrazone, applied in the directions of botanical equipment and methods, nickel organic compounds, applications, etc., to achieve easy industrial production, high sterilization efficiency, and simple synthesis method. Effect
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[0031] The preparation method of ethyl 2-carbonyl butyrate salicylhydrazone nickel complex is characterized in that it comprises the following steps:
[0032] Step a, the preparation of salicylhydrazide;
[0033] Add methyl salicylate and hydrazine hydrate to absolute ethanol at a material ratio of 1:1.1, heat and back-distill to react, evaporate most of the ethanol, cool, stir, and precipitate a white solid, recrystallize with a mixed solvent of water and ethanol , to obtain white needle-like crystals;
[0034] Step b, the preparation of ethyl 2-oxobutyrate salicylhydrazone;
[0035] Add salicylic hydrazide and ethyl acetoacetate into 40mL of absolute ethanol at a material ratio of 1:2, heat and stir in a water bath for back distillation, and react after cooling, white needle-like crystals are precipitated, and the crude product obtained by suction filtration is washed with anhydrous Recrystallized from ethanol to obtain white needle-like crystals;
[0036] Step c, prepara...
Embodiment 1
[0048] Example 1 Field efficacy experiment of ethyl 2-carbonylbutyrate salicyloylhydrazone nickel complex on rice blast
[0049] Experimental principle: The growth rate method is also called the medium dosing method. The method is to mix the test agent with the unsolidified culture medium evenly, and then inoculate the bacteria after cooling and solidification, and use this drug-containing medium to cultivate the bacteria, and judge the inhibitory effect of the test agent by the speed of mycelium growth. bacteria effect.
[0050] The test agent: 2-carbonyl butyrate ethyl salicylhydrazone nickel complex (NiL)
[0051] Pathogen tested: rice blast pathogenic fungus
[0052] Experimental steps: preparation of test agents with different concentration gradients, preparation of pathogenic bacteria, preparation of medium for test agents
[0053] Fight pathogenic bacteria, inoculate, cultivate, inspect
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