Bovine bone collagen peptide preparation method

A collagen peptide, bovine bone technology, applied in the direction of animal/human protein, specific peptide, connective tissue peptide, etc., can solve the problems of long production cycle, easy browning, high energy consumption, etc., to achieve low energy consumption and shorten production Cycle, the effect of broad market prospects

Inactive Publication Date: 2017-03-08
于海峰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The main purpose of the present invention is to solve the extraction rate of bovine collagen peptide prepared by the existing production process by successively adopting the technology of twice crushing, second re-boiling, high-temperature...

Method used

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  • Bovine bone collagen peptide preparation method

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specific Embodiment approach 1

[0042] Specific embodiment one: this embodiment is a kind of preparation method of bovine bone collagen peptide, specifically is finished according to the following steps:

[0043] 1. Screening: According to the raw material acceptance standards and inspection procedures, the purchased fresh beef bone raw materials are inspected;

[0044] 2. Primary crushing: Put the screened fresh beef bones into the bone crusher and crush them into 5-8cm 3 Size and weigh bone fragments;

[0045] 3. Secondary crushing: place the broken bone in step 2 in a bone clay machine for crushing;

[0046] The bone slime machine described in step 3 adopts a 40-mesh stainless steel round hole screen;

[0047] 4. Cleaning: Wash the broken bones in a bone washing machine for 30-40 minutes, and soak them in clean water for 2-3 hours to extract the residual blood and drain them;

[0048] 5. High-temperature extraction: according to the mass ratio of crushed bone pieces to clear water 1: (3.5-4), place in ...

specific Embodiment approach 2

[0069] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is: when extracting at high temperature in step 5, place it in a high-temperature extraction tank at a temperature of 120° C. Extract 2h. Others are the same as the first embodiment.

specific Embodiment approach 3

[0070] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: when reboiling in the second step in step six, put it in the high-temperature extraction tank again according to the mass ratio of bone mud and clear water of 1: 3.1, at a temperature of Extract at 115°C for 2h. Others are the same as those in Embodiment 1 or 2.

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Abstract

The invention provides a bovine bone collagen peptide preparation method, and relates to a protein peptide preparation method. A main purpose of the invention is to solve the problems of low extraction rate, easy browning, high energy consumption and long production period of the existing bovine bone collagen peptide preparation in the prior art by combining a specific compound enzyme preparation through a technology comprising two crushing on bovine bone, secondary reboiling, high temperature cooking and concentration with an ultra-filtration membrane. The method comprises: 1, screening; 2, first crushing; 3, second crushing; 4, washing; 5, high temperature extracting; 6, secondary reboiling; 7, filtration separation; 8, liquid-liquid separation; 9, enzymolysis; 10, sterilization and enzyme inactivation; 11, solid-liquid separation; 12, concentration with an ultra-filtration membrane; 13, spray drying; and 14, packaging so as to obtain the bovine bone collagen peptide. According to the present invention, the preparation method is mainly used for preparing the bovine bone collagen peptide.

Description

technical field [0001] The invention relates to a preparation method of protein peptide. Background technique [0002] At present, the development of food related to protein peptides in my country has the following problems: [0003] 1. The extraction rate of traditional processing technology is low [0004] Collagen peptides, especially bone-like collagen peptides, are prepared by traditional methods such as crushing, high-temperature extraction, and single enzymatic extraction. The general production cycle of this type of preparation process is 16 to 20 hours, and the enzymatic hydrolysis efficiency is low, resulting in collagen peptides Extraction rates have been at a low level. [0005] 2. High production energy consumption [0006] At present, all kinds of collagen peptide production enterprises adopt traditional evaporation concentration method when concentrating the extract, which not only consumes a lot of energy, but also has strict requirements on the steam deli...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/34C07K1/14
Inventor 张根生费英敏程健博
Owner 于海峰
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