Method for measuring total saponins in radix panacis quinquefolii by aid of solid-phase extraction-colorimetric processes

Abstract

The invention discloses a method for measuring total saponins in radix panacis quinquefolii by the aid of solid-phase extraction-colorimetric processes. The method includes steps of (1), pretreating samples; (2), carrying out solid-phase extraction; (3), measuring the total saponins by the aid of colorimetric processes; (4), drawing standard curves and calculating results. The method has the advantages that the total saponins in radix panacis quinquefolii capsules is extracted by the aid of ultrasonic extraction processes, the total saponins in the samples is extracted and purified by the aid of solid-phase extraction processes, accordingly, grease, impurity saccharides and the like can be effectively separated from the total saponins, and extracted target products are high in stability; the content of the total saponins is analyzed and measured by the aid of the vanillin-perchloric acid-glacial acetic acid colorimetric processes in 40 min, and the results are good in stability and high in precision; the method is simple, convenient and feasible as compared with the traditional method, is high in repeatability and recovery rate, is applicable to analyzing the content and the quality of the total saponins in healthcare foods represented by the radix panacis quinquefolii and has important reference significance on quality control of the healthcare foods.

Description

technical field [0001] The invention relates to the technical field of health food detection, in particular to a solid-phase extraction-colorimetric method for determining total saponins in American ginseng. Background technique [0002] American ginseng (Panaxquinquefolium L), also known as American ginseng, American ginseng, and American ginseng, is Araliaceae ginseng. Yin reduces fire, replenishes qi and promotes fluid production. With the improvement of people's living standards, some products with health functions represented by American ginseng emerge in an endless stream. Most of these health foods on the market use the roots of American ginseng as raw materials, which are made into American ginseng lozenges, American ginseng lozenges after crushing or extraction. Tea or American ginseng capsules, etc., the health function is measured by the total saponin content. The research methods of its quantitative analysis mainly include gravimetry, colorimetry, near-infrared...

AI Technical Summary

Problems solved by technology

The gravimetric analysis process takes a long time, the operation is cumbersome, and the consumption of reagents is large. Generally, the obtained saponins are mixed with a certain amount of impurities, and the error is relatively large. Severe overlap, weak signal, coupled with instrument noise, baseline drift, stray light and other factors, severely limit the accuracy of its research; HPLC has high requirements for equipment, and the HPLC method is suitable for the determination of a single ginsenoside

At present, most domestic researches are determined according to the method of "Determination of Total Saponins in Health Food" in "Technical Specifications for Inspection and Evaluation of Health Food (2003 Edition)", but there are reports in the literature that the reproducibility of the analysis results measured according to the standard method The comparability is poor, and there are outliers in some concentration levels

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