Dendrobium officinale complex bacterial agent

A technology of Dendrobium candidum and compound bacterial agent, which is applied in the direction of bacteria, fungi, and microbial-based methods, can solve the problems of endangered natural resources, strict environmental conditions, and slow growth, and achieves improved resistance to extreme environments and high efficiency. Defense ability, enhance the effect of self-repair

Inactive Publication Date: 2017-03-15
云南山里红生物科技有限公司
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Problems solved by technology

[0003] At present, due to the low natural reproduction rate of Dendrobium candidum, strict requirements on environmental conditions, and long-term mining by farmers in production areas, natural resources are on the verge of extinction.
However, because the seeds of Dendrobium candidum are tiny and have no endosperm, it is not conducive to obtaining

Method used

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  • Dendrobium officinale complex bacterial agent

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Embodiment Construction

[0025] 1. Isolation and purification of actinomycetes in roots

[0026] (1) Select the healthy and fresh Dendrobium candidum nutrient root system, wrap the root sample with gauze and rinse it with running tap water.

[0027] (2) Disinfect in 75% ethanol for 20 seconds, rinse with sterile water for 2-3 times, then disinfect with 0.1% mercury chloride solution for 20 seconds, rinse with sterile water for 4-5 times, use sterile filter paper to absorb the water on the surface of the root sample Dry.

[0028] (3) Set up a blank control test, that is, put the sterilized root samples on Gaoshi No. 1 medium and roll them several times.

[0029] (4) Put the sterilized root samples on the surface, grind them into a homogenous slurry in an ultra-clean workbench, and microwave them for 30 seconds. Because the endophytic actinomycetes are isolated, and the root actinomycetes are difficult to isolate, so it must be fully ground to increase the isolation types of endophytic actinomycetes; ...

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Abstract

The invention belongs to the technical field of biological bacterial fertilizers and particularly relates to a dendrobium officinale complex bacterial agent. The complex bacterial agent is prepared by steps of separation and purification of in-root actinomycetes, screening of antagonistic actinomycetes, screening of mycorrhiza fungi growth promoting actinomycetes, determination of species status of the growth promoting actinomycetes and growth promotion of dendrobium officinale clonal seedlings by synergy of optimized mycorrhiza fungi and the actinomycetes. By synergy of dendrobium officinale mycorrhiza fungi and the in-root actinomycetes for obtaining the complex bacterial agent, dendrobium officinale cells can be stimulated to generate efficient defense capability, self repairing, disease resistance, extreme environment resistance of dendrobium officinale can be improved, the survival rate of the dendrobium officinale clonal seedlings is increased, growth of the dendrobium officinale clonal seedlings is promoted, and large-scale production of high-quality dendrobium officinale seedlings is supported technically.

Description

technical field [0001] The invention belongs to the technical field of biological bacterial fertilizer, and in particular relates to a composite bacterial agent of dendrobium officinale. Background technique [0002] Dendrobium officinale is a perennial epiphyte of the genus Dendrobium orchidaceae, which is widely used in research and development in the field of medicine. Modern medical research shows that Dendrobium candidum has medicinal effects such as nourishing the lungs and clearing heat, nourishing yin and nourishing the stomach, clearing the liver and improving eyesight, resisting cancer, and lowering blood sugar. As the best product for health care, Dendrobium officinale has been highly praised by medical scientists of all ages. [0003] At present, due to the low natural reproduction rate of Dendrobium officinale, strict requirements on environmental conditions, and long-term mining by farmers in production areas, natural resources are on the verge of extinction. ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C05F11/08C12R1/465
CPCC12N1/20C05F11/08C12N1/14
Inventor 丁雅迪范志伟岳健苏娟李昆华李锋
Owner 云南山里红生物科技有限公司
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