Dendrobium officinale complex bacterial agent
A technology of Dendrobium candidum and compound bacterial agent, which is applied in the direction of bacteria, fungi, and microbial-based methods, can solve the problems of endangered natural resources, strict environmental conditions, and slow growth, and achieves improved resistance to extreme environments and high efficiency. Defense ability, enhance the effect of self-repair
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[0025] 1. Isolation and purification of actinomycetes in roots
[0026] (1) Select the healthy and fresh Dendrobium candidum nutrient root system, wrap the root sample with gauze and rinse it with running tap water.
[0027] (2) Disinfect in 75% ethanol for 20 seconds, rinse with sterile water for 2-3 times, then disinfect with 0.1% mercury chloride solution for 20 seconds, rinse with sterile water for 4-5 times, use sterile filter paper to absorb the water on the surface of the root sample Dry.
[0028] (3) Set up a blank control test, that is, put the sterilized root samples on Gaoshi No. 1 medium and roll them several times.
[0029] (4) Put the sterilized root samples on the surface, grind them into a homogenous slurry in an ultra-clean workbench, and microwave them for 30 seconds. Because the endophytic actinomycetes are isolated, and the root actinomycetes are difficult to isolate, so it must be fully ground to increase the isolation types of endophytic actinomycetes; ...
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