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Activation fluorescence latex microsphere for gastrin 17 fluorescence immunochromatographic assay

A technology of fluorescent latex microspheres and fluorescent immunochromatography, which is applied in the analysis of materials, luminescent materials, and biomaterials. It can solve the problems of reduced detection sensitivity, uneven dispersion, and chromatographic failure, and achieve stable detection results. Accurate results and easy operation

Active Publication Date: 2017-03-15
BEIJING ELCOTEQ BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is: after drying, the fluorescent latex microspheres of the fluorescent immunochromatographic test strips are easy to agglomerate and unevenly dispersed, resulting in chromatography failure or reduced detection sensitivity

Method used

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  • Activation fluorescence latex microsphere for gastrin 17 fluorescence immunochromatographic assay
  • Activation fluorescence latex microsphere for gastrin 17 fluorescence immunochromatographic assay
  • Activation fluorescence latex microsphere for gastrin 17 fluorescence immunochromatographic assay

Examples

Experimental program
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Effect test

Embodiment 1

[0048] 1) Dissolve the surfactant (2mg polyethylene glycol monolaurate, 3.5mg sodium stearate, 0.5mg sodium lauryl dimethylene carbamate and 4mg sodium cocoyl glutamate) in 0.5M pH9.6 carbonic acid buffer (H value to 9.6), then add in 0.1ml of DMF, add N,N'-dicyclohexylcarbodiimide (DCC) 1mg and 0.6 mg N-hydroxysuccinimide (NHS) and stirred at room temperature for 3 hours.

[0049] 2) Adjust the pH value of 1ml of fluorescent latex microsphere solution containing 1% solids on the amino surface to 9.6 with 0.5M carbonic acid buffer solution of pH 9.6, then add it to step 1), and continue to stir and react for 3 hours. Acquired activated latex microspheres.

[0050] 3) Use a high-speed centrifuge to centrifuge the fluorescent latex microsphere reaction solution at high speed, remove the supernatant of the reaction solution, and redissolve the fluorescent latex microspheres with 1mL microsphere buffer to form a marker working solution.

[0051] Microsphere phosphate buffer prep...

Embodiment 2

[0053] The operation method of this example is similar to Example 1, except that the surfactant in step 1) is: 1 mg polyethylene glycol monolaurate, 5 mg sodium stearate, 1 mg dodecyl dimethylene Sodium Carbamate and 5mg Sodium Cocoyl Glutamate.

Embodiment 3

[0055] The operation method of this example is similar to that of Example 1, except that the surfactant in step 1) is: 2.5 mg polyethylene glycol monolaurate, 5 mg sodium stearate, 1.5 mg lauryl di Sodium methylene carbamate and 5 mg sodium cocoyl glutamate.

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Abstract

The invention discloses activated fluorescence latex microspheres and a test strip for gastrin 17 fluorescence immunochromatographic assay. A preparation method of the activated fluorescence latex microspheres for a gastrin 17 fluorescence immunochromatographic assay test strip comprises the following steps: 1) adding a surfactant into a buffer solution of which the pH value is 8-10, and further adding dimethyl formamide, N,N'-dicyclohexylcarbodiimide and N-hydroxysuccinimide; 2) adjusting the pH value of the buffer solution to be 8-10 by using a dispersion liquid of fluorescence latex microspheres on surface of amino, adding into the mixture obtained in the step 1), reacting under stirring, and after the reaction is completed, centrifuging to remove supernate, thereby obtaining the surface activated fluorescence latex microspheres, wherein the surfactant comprises poly(ethylene glycol) monolaurate, stearate, dodecyl dimethylene amino diformate and cocoyl glutamate in a weight ratio of (1-5):(5-10):(0.5-3):(5-10).

Description

technical field [0001] The invention belongs to the technical field of fluorescence immunochromatography, and in particular relates to an activated fluorescent latex microsphere for a gastrin 17 fluorescence immunochromatography test strip and its preparation and application. Background technique [0002] Fluorescence immunochromatography test strips (reagent cards) are widely used in the field of quick detection because of their fast and convenient characteristics. The analyte antibody is labeled with fluorescent latex microspheres, and the latex microspheres have surface electron repulsion and van der Waals forces in the liquid phase to keep the latex particles in the liquid phase evenly distributed. However, after spraying on the glass cellulose film and drying, as the solution is evaporated to dryness, the surface tension pushes the latex particles infinitely closer together, resulting in the disappearance of the intermolecular repulsion, and the increase of the van der ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/543G01N33/558G01N33/577G01N33/74C09K11/06
CPCC09K11/06C09K2211/14G01N33/533G01N33/54313G01N33/558G01N33/577G01N33/74G01N2800/06
Inventor 林斯
Owner BEIJING ELCOTEQ BIO TECH
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