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Preparing method for nano-composite fiber film with functions of carrying medicine and killing cancer cells

A technology of nano-composite fibers and cancer cells, applied in the field of nano-composite fiber films and their preparation, can solve the problems of difficulty in obtaining tumor cells and the like

Active Publication Date: 2017-03-22
UNIV OF SCI & TECH BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is very difficult to obtain tumor cells from peripheral blood samples

Method used

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  • Preparing method for nano-composite fiber film with functions of carrying medicine and killing cancer cells
  • Preparing method for nano-composite fiber film with functions of carrying medicine and killing cancer cells
  • Preparing method for nano-composite fiber film with functions of carrying medicine and killing cancer cells

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preparation example Construction

[0025] The invention discloses a method for preparing a nanocomposite fiber film with dual functions of drug loading and killing cancer cells. The method adopts electrospinning technology to prepare a nanocomposite fiber film loaded with antitumor drugs, and performs carboxyl modification on the nanocomposite fiber film. properties, and then modify the anti-epithelial cell adhesion molecule protein on the surface, which specifically includes the following steps:

[0026] Step 1, preparing gold nanoparticles loaded with antitumor drugs;

[0027] Step 2, mixing the gold nanoparticles prepared in step 1 with the polymer solution and electrospinning to prepare a nanocomposite fiber film;

[0028] Step 3: Carboxyl-modify the nanocomposite fiber film obtained in step 2, and then modify anti-EpCAM to obtain a nanocomposite fiber film with dual functions of drug loading and killing cancer cells.

[0029] The first step is as follows: add 0.02-0.04 μl methyl thioglycolate to 20-40 mL ...

Embodiment 1

[0036] (1) Preparation of gold nanoparticles

[0037] 100 mL containing 0.01% HAuCl 4 ·3H 2The aqueous O solution was heated and stirred vigorously until boiling. Add 4 mL of 1% sodium citrate to the boiling solution. After the color of the solution was observed to change from dark blue to wine red, the solution was reboiled with stirring. Then remove the heating source and continue to stir, so that the colloidal solution of gold nanoparticles (AuNPs) with a diameter of about 10-20nm is produced.

[0038] (2) Doxorubicin modified onto gold nanoparticles

[0039] Using 40 mL of the above colloidal solution of gold nanoparticles, add 0.04 μL of methylthioglycolate (MTG), and react in the dark. Continue to inject 0.1 μL of hydrazine hydrate solution into the above colloidal gold solution, stir at 50°C, dialyze in pure water for 3 days, and then freeze-dry the solution to obtain gold nanoparticle powder modified with hydrazine hydrate (AuNPs-NH-NH2 ). Take 1 mg of the prepa...

Embodiment 2

[0047] (1) The doxorubicin (Au-DOX) modified on the Au nanoparticles was evenly distributed in the polyacrylonitrile solution for co-spinning to obtain polyacrylonitrile nanofibers wrapped with Au-DOX. called composite nanofibers.

[0048] (2) Carboxylation modification of composite nanofibers: soak polyacrylonitrile (PAN) nanofibers in a concentrated NaOH solution with a mass fraction of 20%, and hydrolyze at 50°C. The surface of the hydrolyzed nanofibers turned orange-red and emitted a pungent odor. Then put the hydrolyzed and oxidized nanofiber membrane into a hydrochloric acid solution with a mass fraction of 4%, and protonize it at 37° C. for 12 hours. The orange-red color on the surface of the nanofiber fades and turns white again. Finally, the reacted nanofiber membrane is washed with a large amount of water, put into a vacuum drying oven to make it completely dry, and carboxyl-functionalized polyacrylonitrile composite nanofibers are obtained.

[0049] (3) Composite ...

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Abstract

The invention provides a preparing method for a nano-composite fiber film with the functions of carrying medicine and killing cancer cells. The nano-composite fiber film has the function of capturing and killing circulating cancer cells. The preparing method includes the steps that nano-composite fibers are prepared into the nano-composite fiber film with carboxyl functional groups or carboxyl functional groups generated through modification with the electrostatic spinning technology, the film is carried with anticancer medicine, and then anti-epithelial cell adhesion molecule protein (anti-EpCAM) is grafted to the surface of the nano-fiber film. The nano-composite fiber system prepared with the method has the advantage that the dual functions that the circulating cancer cells can be captured and killed at the same time are achieved; the nano-composite fiber system carrying the anticancer medicine has the advantage that circulating cancer is specifically captured through the specificity; the nano-composite fiber system can automatically release the anticancer medicine through the PH value of the ambient environment of the cancer cells, and the aim that the circulating cancer cells are killed is achieved.

Description

technical field [0001] The invention relates to the technical field of nanometer functional materials, in particular to a nanocomposite fiber film capable of loading drugs and killing cancer cells and a preparation method thereof. Background technique [0002] Cancer is one of the greatest threats to human health and life, and conquering cancer has always been the dream of all mankind. With the rapid development of nanotechnology, scientists have designed and prepared a series of drug controlled release systems based on nanomaterials, which are used to kill tumor cells and treat cancer. The most deadly tumor is not the "static" tumor cells, but the "flowing" and "spreadable" circulating tumor cells (CTCs). It has been reported that more than 90% of cancer deaths are caused by the spread of tumor cells from the initial tumor location to tissues and organs throughout the body. It can be seen that designing and preparing a drug controlled release system that can target and ki...

Claims

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Application Information

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IPC IPC(8): A61K9/70A61K47/52A61K47/32A61K47/36A61K47/42A61K31/704A61K31/337A61K31/17A61P35/00A61M31/00D06M13/21D06M13/402D06M13/328D06M15/15D04H1/728D06M101/26D06M101/28
CPCA61K9/7007A61K31/17A61K31/337A61K31/704A61K47/32A61K47/36A61K47/42A61M31/00D04H1/728D06M13/21D06M13/328D06M13/402D06M15/15D06M2101/26D06M2101/28A61K2300/00
Inventor 温永强张淼李延生
Owner UNIV OF SCI & TECH BEIJING
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