Extracellular aldonic acid lactonase PoALAC and application thereof

A technology of lactonase and aldonic acid, applied in the fields of application, hydrolase, sugar production, etc.
CN106520726AActive Publication Date: 2017-03-22SHANDONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANDONG UNIV
Publication Date
2017-03-22

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Abstract

The invention discloses an extracellular aldonic acid lactonase PoALAC. An amino acid sequence thereof is as shown in SEQ ID NO.1 and a nucleotide sequence thereof is as shown in SEQ ID NO.2. The invention also discloses an application of the extracellular aldonic acid lactonase PoALAC in synergic degradation of celluloses. The specific enzyme activity can reach up to 173009IU / mg. After the extracellular aldonic acid lactonase PoALAC is added into a cellulose system, the extracellular aldonic acid lactonase PoALAC has a synergic effect with polysaccharide monooxygenase and beta-glucosidase and the degrading effects for different pre-treated natural lignocellulose substrates are promoted, so that the extracellular aldonic acid lactonase PoALAC has huge application potential and economic benefit in the industries of biological energy source and bio-based chemicals.
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Description

Technical field

[0001] The invention relates to an extracellular aldonic lactonase, in particular to an extracellular aldonic lactonase PoALAC and its application, and belongs to the field of genetic engineering. Background technique

[0002] Lignocellulosic biomass is the most abundant renewable resource in the world and can be used to produce bioethanol fuel and other value-added products. The degradation of cellulose requires the synergistic action of endoglucanase cellobiohydrolase to convert it into cellobiose, which is then hydrolyzed into glucose by β-glucosidase. In recent years, it has been discovered that a lysing polysaccharide monooxygenase (LPMOs) can break the cellulose chain through oxidation, thereby synergistically degrading lignocellulose with other cellulolytic enzymes. Studies have shown that the hydrolysates of polysaccharide monooxygenase include cellooligosaccharides, aldonic acid lactones and aldonic acid. As we all know, aldonic acid lactone has inhibit...

Claims

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