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High-yield preparation method for active polysaccharides of wheat bran

A technology for wheat bran and active polysaccharide, which is applied in the field of plant polysaccharide extraction, can solve the problems of affecting the biological activity of wheat bran polysaccharide and destroying the polysaccharide structure, and achieves the effects of mild extraction conditions, good activity and simple extraction operation steps.

Active Publication Date: 2017-03-22
HUAIYIN TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are some problems in these extraction methods, for example: the acid-base extractant will destroy the polysaccharide structure to a certain extent, thereby affecting the biological activity of wheat bran polysaccharides

Method used

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  • High-yield preparation method for active polysaccharides of wheat bran
  • High-yield preparation method for active polysaccharides of wheat bran
  • High-yield preparation method for active polysaccharides of wheat bran

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Wheat bran pretreatment: After the wheat bran is mechanically crushed, impurities are removed, and the removed wheat bran is heated and dried in an oven at 30°C until the moisture content of the wheat bran is within 3%. Then, the wheat bran is further pulverized by an ultrafine pulverizer until the particle size is 500 mesh, and the wheat bran ultrafine powder is obtained;

[0041] (2) Degreasing treatment of wheat bran: according to the ratio of 1:6 of the mass grams of wheat bran superfine powder and the volume milliliter of sherwood oil, sherwood oil is added in wheat bran superfine powder; The obtained mixed system was stirred in a water bath at 30°C for 4 hours, degreased, and then the defatted wheat bran superfine powder was dried in an oven at 40°C;

[0042](3) Wheat bran crude polysaccharide extraction: take by weighing wheat bran superfine powder after degreasing, and by the mass gram ratio of the wheat bran superfine powder after degreasing and water is th...

experiment example 1 Embodiment 1

[0045] Experimental example 1 Example 1 Wheat bran crude polysaccharide and wheat bran active polysaccharide sample test

[0046] 1) Determination of polysaccharide content in wheat bran crude polysaccharide according to phenol-sulfuric acid method:

[0047] (1) Drawing of the standard curve: Accurately weigh 10 mg of glucose dried at 105°C to constant weight, and dilute to a 100 mL volumetric flask with distilled water to obtain a standard solution; dilute the standard solution to a mass concentration of 50, 100, 150, 200, 250 , 300, and 350 mg / mL solutions, absorb 0.2 mL of the diluent, add 0.4 mL of 5% phenol by mass fraction, and quickly add 2 mL of concentrated sulfuric acid after mixing. Make a blank control; make a standard curve with the polysaccharide concentration as the abscissa (X) and the absorbance as the ordinate (Y);

[0048] (2) Sample determination: take by weighing 40-50 mg of the wheat bran crude polysaccharide sample extracted in Example 1, distilled wate...

Embodiment 2

[0062] (1) Wheat bran pretreatment: After the wheat bran is mechanically crushed, impurities are removed, and the removed wheat bran is heated and dried in a 60°C oven until the moisture content of the wheat bran is within 6%. Then, the wheat bran is further pulverized by an ultrafine pulverizer until the particle size is 2000 mesh sieves to obtain wheat bran ultrafine powder;

[0063] (2) Degreasing treatment of wheat bran: according to the ratio of 1:4 of the mass grams of wheat bran superfine powder and the volume milliliter of sherwood oil, sherwood oil is added in wheat bran superfine powder; Then, will The resulting mixed system was stirred for 4 hours in a water bath at 30°C for degreasing, and then the degreased superfine wheat bran powder was dried in an oven at 40°C;

[0064] (3) Wheat bran crude polysaccharide extraction: take by weighing wheat bran superfine powder after degreasing, and by the mass gram ratio of the wheat bran superfine powder after degreasing and ...

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Abstract

The invention provides a high-yield preparation method for active polysaccharides of wheat bran. In the method provided by the invention, a wheat bran superfine powder is taken as a raw material, degreased through petroleum ether, and then subjected to cellulase extraction, alcohol precipitation, starch removal and protein removal to prepare the crude polysaccharides of wheat bran. The reaction conditions of the method provided by the invention are moderate, acids and bases damaging a polysaccharide structure are avoided, the overall preparation process is simple, and the method is a practical, efficient and high-yield method for preparing the polysaccharides of wheat bran. Meanwhile, the polysaccharides of wheat bran, which are prepared by the method provided by the invention, have the advantages of being high in sugar content, high in purity, high in bioactivity and the like.

Description

technical field [0001] The invention relates to the field of extracting plant polysaccharides, in particular to a method for preparing high-yield active polysaccharides from wheat bran. Background technique [0002] Wheat is one of the earliest cultivated crops in the world, belonging to the Gramineae monocotyledons plant. The caryopsis of wheat is the main part of human consumption. After being ground into flour, it can be made into steamed buns, noodles and other foods. Bran is recorded in "Compendium of Materia Medica" that "bran is wheat skin, which is the same sex as floating wheat, and the antiperspirant effect is second only to floating wheat, and it has no meat." Bran can also be used to clear away heat and eliminate phlegm. Softening and dispelling stagnation and tonifying deficiency and suppressing sweat. In my country, the annual output of wheat bran is as high as more than 20 million tons, accounting for about 20% of wheat, but more than 85% of wheat bran is us...

Claims

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Application Information

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IPC IPC(8): C12P19/04
CPCC12P19/04
Inventor 胡卫成沈婷王新风宋虎卫朱翠玲
Owner HUAIYIN TEACHERS COLLEGE
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