High-yield preparation method for active polysaccharides of wheat bran

A technology for wheat bran and active polysaccharide, which is applied in the field of plant polysaccharide extraction, can solve the problems of affecting the biological activity of wheat bran polysaccharide and destroying the polysaccharide structure, and achieves the effects of mild extraction conditions, good activity and simple extraction operation steps.

Active Publication Date: 2017-03-22
HUAIYIN TEACHERS COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are some problems in these extraction methods, for example: the acid-base extractant will destroy the poly

Method used

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  • High-yield preparation method for active polysaccharides of wheat bran
  • High-yield preparation method for active polysaccharides of wheat bran
  • High-yield preparation method for active polysaccharides of wheat bran

Examples

Experimental program
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Example Embodiment

[0039] Example 1:

[0040] (1) Wheat bran pretreatment: After the wheat bran is mechanically crushed, impurities are removed, and the removed wheat bran is heated and dried in an oven at 30°C until the moisture content of the wheat bran is within 3%. Then, the wheat bran is further pulverized by an ultrafine pulverizer to a particle size of 500 meshes to obtain an ultrafine wheat bran powder;

[0041] (2) Degreasing treatment of wheat bran: According to the ratio of the mass grams of wheat bran superfine powder to the volume milliliters of petroleum ether at a ratio of 1:6, add petroleum ether to the wheat bran superfine powder; The resulting mixed system was stirred for 4 hours under a water bath at 30°C for degreasing, and then the defatted wheat bran ultrafine powder was dried in an oven at 40°C;

[0042] (3) Extraction of crude polysaccharides from wheat bran: Weigh defatted wheat bran superfine powder, and transfer the defatted wheat bran superfine powder to water in a ratio of...

Example

[0045] Experimental example 1 Example 1 Wheat bran crude polysaccharide and wheat bran active polysaccharide sample test

[0046] 1) Determine the polysaccharide content in wheat bran crude polysaccharides according to the phenol-sulfuric acid method:

[0047] (1) Drawing the standard curve: accurately weigh 10 mg glucose dried to constant weight at 105°C, and distilled water to a 100mL volumetric flask to obtain a standard solution; dilute the standard solution to a mass concentration of 50, 100, 150, 200, 250 , 300, and 350 mg / mL solution, respectively draw 0.2 mL of the diluent, add 0.4 mL of 5% phenol by mass fraction, and quickly add 2 mL of concentrated sulfuric acid after mixing. After mixing, let it stand at room temperature for 30 minutes, and measure the absorbance at 490nm wavelength with distilled water Make a blank control; use the polysaccharide concentration as the abscissa (X) and absorbance as the ordinate (Y) to make a standard curve;

[0048] (2) Sample determinat...

Example Embodiment

[0061] Example 2:

[0062] (1) Wheat bran pretreatment: the wheat bran is mechanically crushed to remove impurities, and the removed wheat bran is heated and dried in an oven at 60°C until the moisture content of the wheat bran is within 6%. Then, the wheat bran is further pulverized by an ultrafine pulverizer to a particle size of 2000 mesh sieve to obtain wheat bran ultrafine powder;

[0063] (2) Degreasing treatment of wheat bran: According to the ratio of the weight of the wheat bran superfine powder to the volume of petroleum ether in a ratio of 1:4, petroleum ether is added to the wheat bran superfine powder; The resulting mixed system was stirred for 4 hours under 30°C water bath for degreasing, and then the defatted wheat bran ultrafine powder was dried in an oven at 40°C;

[0064] (3) Extraction of crude polysaccharides from wheat bran: Weigh defatted wheat bran ultrafine powder, and transfer the defatted wheat bran ultrafine powder to water at a mass ratio of 1:25 to the w...

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Abstract

The invention provides a high-yield preparation method for active polysaccharides of wheat bran. In the method provided by the invention, a wheat bran superfine powder is taken as a raw material, degreased through petroleum ether, and then subjected to cellulase extraction, alcohol precipitation, starch removal and protein removal to prepare the crude polysaccharides of wheat bran. The reaction conditions of the method provided by the invention are moderate, acids and bases damaging a polysaccharide structure are avoided, the overall preparation process is simple, and the method is a practical, efficient and high-yield method for preparing the polysaccharides of wheat bran. Meanwhile, the polysaccharides of wheat bran, which are prepared by the method provided by the invention, have the advantages of being high in sugar content, high in purity, high in bioactivity and the like.

Description

technical field [0001] The invention relates to the field of extracting plant polysaccharides, in particular to a method for preparing high-yield active polysaccharides from wheat bran. Background technique [0002] Wheat is one of the earliest cultivated crops in the world, belonging to the Gramineae monocotyledons plant. The caryopsis of wheat is the main part of human consumption. After being ground into flour, it can be made into steamed buns, noodles and other foods. Bran is recorded in "Compendium of Materia Medica" that "bran is wheat skin, which is the same sex as floating wheat, and the antiperspirant effect is second only to floating wheat, and it has no meat." Bran can also be used to clear away heat and eliminate phlegm. Softening and dispelling stagnation and tonifying deficiency and suppressing sweat. In my country, the annual output of wheat bran is as high as more than 20 million tons, accounting for about 20% of wheat, but more than 85% of wheat bran is us...

Claims

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Application Information

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IPC IPC(8): C12P19/04
CPCC12P19/04
Inventor 胡卫成沈婷王新风宋虎卫朱翠玲
Owner HUAIYIN TEACHERS COLLEGE
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