Device and method for dispersive solid-phase extraction- magnetic needle desorption electricity rising atomizing ionized mass spectrometry of magnetic nanoparticles

Abstract

The invention discloses a device and a method for dispersive solid-phase extraction- magnetic needle desorption electricity rising atomizing ionized mass spectrometry of magnetic nanoparticles. The method comprises steps of 1), dispersing magnetic nanoparticles in a solution to be tested, and performing ultrasound extraction; 2), inserting a magnetic needle in the solution to be tested, and collecting magnetic nanoparticles; 3), fixing the magnetic needle in an analysis device, starting power source and an injector, and dropwise adding desorption fluid on the surface of the magnetic needle; 4) adjusting power voltage, and adjusting the distance between the magnetic needle and a sample inlet of a mass spectrometer according to an atomizing effect formed in a camera, so as to obtain a stable signal; 5), collecting and analyzing a mass spectrum signal, and obtaining an analysis result. The device and method can effectively solve the combination problem of magnetic dispersive solid-phase extraction and direct desorption electricity rising atomizing spectrometry analysis. The magnetic nanoparticles absorbing materials can compound according to the type of a target, and the technology has important meaning for improving the analysis efficiency.

Description

technical field [0001] The invention relates to a magnetic nanoparticle dispersion solid phase extraction-magnetic needle desorption nanoelectrospray ionization mass spectrometry device and method. [0002] technical background [0003] The sample pretreatment of modern chemical analysis faces many challenges such as complex sample matrix, low concentration of target compounds and small sample volume. Traditional pretreatment methods, such as column solid-phase extraction and liquid-liquid extraction, have disadvantages such as long pretreatment time, large consumption of organic solvents, and large potential loss of target substances. How to realize the combination of efficient extraction and instrumental analysis and reduce the analysis cost is one of the main problems to be solved in chemical analysis at present. The direct combination of mass spectrometry and sample pretreatment can effectively shorten the analysis time. For example, the combination of solid-phase microe...

AI Technical Summary

Problems solved by technology

The direct combination of mass spectrometry and sample pretreatment can effectively shorten the analysis time. For example, the combination of solid-phase microextraction and analytical electrospray technology can achieve high-sensitivity analysis of the target. The transfer speed between the extraction probe and the extraction probe is slow, and there are disadvantages such as long extraction time and uneven distribution of the target on the surface of the extraction head, which easily reduces the accuracy and sensitivity of the detection

The magnetic nanoparticle dispersion solid phase extraction technology disperses magnetic nanoparticles in the solution system, and can realize the rapid transfer of target molecules between the solution system and the nanoparticles by ultrasound. However, since the magnetic nanoparticles cannot be directly combined with the mass spectrometer, it is necessary to After the target molecules are analyzed from the surface of the particle, the sample is injected after further concentration and separation, which cannot simplify the operation process

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