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Method for decreasing mildewing rate of elymus sibiricus seeds in germination stage

A technology of old mango wheat seeds and germination period, which is applied in the fields of seed immunity, seed and rhizome treatment, botanical equipment and methods, etc., can solve the problems of Qinghai-Tibet Plateau ecosystem loss, herbivore threat, poor sterilization effect, etc., and achieve reduction Seed mildew rate, enhanced germination potential and germination rate, less damage to vigor

Inactive Publication Date: 2017-03-29
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the treatment of seed fungus, the traditional method mainly uses warm soup soaking or chemical agent seed dressing to kill the seed fungus before sowing, but the bactericidal effect is poor
At the same time, chemical seed dressing may pose a potential threat to local birds and other herbivores in the food chain, and may also cause irreparable damage to the already fragile ecosystem of the Qinghai-Tibet Plateau

Method used

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  • Method for decreasing mildewing rate of elymus sibiricus seeds in germination stage
  • Method for decreasing mildewing rate of elymus sibiricus seeds in germination stage
  • Method for decreasing mildewing rate of elymus sibiricus seeds in germination stage

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Comparative Example 1

[0036] The method of reducing the mildew rate of old mango wheat seeds during germination is as follows:

[0037] 1. Heat the water bath to 40±1℃ and keep it warm for later use, and prepare 200mL of distilled water at 40±1℃ for later use;

[0038] 2. Put the old awn wheat seeds in a beaker, and add the prepared 40±1°C distilled water to submerge the seeds;

[0039] 3. Place the beaker with seeds and distilled water in a water bath for constant temperature initiation;

[0040] 4. Filter the seeds after 15 minutes and place them in a sterile environment to dry naturally;

[0041] 5. Put the dried seeds in a beaker, add sterile water to soak the seeds, take out the seeds after 12 hours, and place the seeds evenly in a 9cm petri dish covered with two layers of sterilized filter paper, 50 per dish 1 seed, 4 replicates, add 6ml of sterile water to each dish, seal the Petri dish with a sealing film and place it in the dark at 25±1°C for cul...

Embodiment 2 comparative example 2

[0043] The method of reducing the mildew rate of old mango wheat seeds during germination is as follows:

[0044] 1. Heat the water bath to 40±1°C and keep it warm for later use, and prepare 200mL of distilled water at 40°C for later use;

[0045] 2. Put the old awn wheat seeds in a beaker, and add the pre-prepared 40°C distilled water to cover the seeds;

[0046] 3. Place the beaker with seeds and distilled water in a water bath for constant temperature initiation;

[0047] 4. Filter the seeds after 15 minutes and place them in a sterile environment to dry naturally;

[0048]5. Put the dried seeds in a beaker, add pre-sterilized PDB medium to soak the seeds, take out the seeds after 12 hours, and place the seeds evenly in a 9cm petri dish covered with two layers of sterilized filter paper , 50 seeds per dish, 4 repetitions, add 6ml sterile water to each dish, seal the Petri dish with a sealing film and place it in the dark at 25±1°C for cultivation, observe and count the ge...

Embodiment 3

[0050] The method for reducing the mildew rate of old mango wheat seeds in the germination period of the present invention is as follows:

[0051] 1. Heat the water bath to 40±1°C and keep it warm for later use, and prepare 200mL of distilled water at 40°C for later use;

[0052] 2. Put the old awn wheat seeds in a beaker, and add the pre-prepared 40°C distilled water to cover the seeds;

[0053] 3. Place the beaker with seeds and distilled water in a water bath for constant temperature initiation;

[0054] 4. Filter the seeds after 15 minutes and place them in a sterile environment to dry naturally;

[0055] 5. Neotyphodium Preparation of sp. endophytic fungus liquid: the activated Neotyphodium Inoculate the quality of sp. endophytic fungus strains into sterilized PDB liquid medium; cultivate on a constant temperature culture shaker with a rotation speed of 200 rpm and a temperature of 23°C; after 8 weeks, the cultured endophytic fungi Bacteria solution was subjected ...

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Abstract

The invention provides a method for decreasing the mildewing rate of elymus sibiricus seeds in the germination stage. The method comprises the steps that the elymus sibiricus seeds are subjected to priming in water at the constant temperature of 40+ / -1 DEG C and then subjected to natural dehydration, and the seeds obtained after natural dehydration are subjected to seed soaking treatment for a period of time with Neotyphodium sp. liquid. The method has the advantages that safety is achieved, wherein Neotyphodium sp. separated from gramineous plants is utilized, and therefore not only can high efficiency of the Neotyphodium sp. be guaranteed to the maximum extent, but also safety and non-toxicity to the ecological environment can be guaranteed; high efficiency is achieved, wherein warm water bath priming is conducted on the seeds while seed-carried fungi are killed, and therefore high efficiency and rapidness are achieved; operation is easy and convenient, wherein the steps are simple and comprise constant-temperature water bath priming, seed dehydration and seed soaking through fungus liquid; low damage is generated to seed vitality, wherein due to the fact that the constant-temperature water bath priming temperature is low and the treatment time is short, no influence can be generated to seed germination, the germination potential of the seeds can be improved to a certain extent, and the germination rate of the seeds can be increased to a certain extent; the mildewing rate of the seeds is significantly decreased.

Description

technical field [0001] The invention belongs to the technical field of interaction between microorganisms and plants and forage planting, and in particular relates to a method for reducing the mildew rate of old awn wheat seeds in the germination stage. Background technique [0002] old awn wheat ( Elymus sibiricus ), also known as Elymus siberia, is a perennial sparse bush-type medium-xerophyte of Gramineae (Gramineae) wheat family (Triticeae). It has excellent characteristics such as strong adaptability, cold resistance, high crude protein content, good palatability and easy cultivation. It is widely used in the establishment of artificial grassland and grazing grassland in the alpine region of Northwest my country. It is an excellent forage grass widely cultivated in the Qinghai-Tibet Plateau region of my country. It is often used as supplementary feed for livestock such as yaks and Tibetan sheep. It plays an important role in grassland animal husbandry. At the same time, ...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01C1/08A01N63/04A01P21/00A01P3/00
CPCA01C1/00A01C1/08A01N63/30
Inventor 李秀璋李春杰魏学凯刘静
Owner LANZHOU UNIVERSITY
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