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A preparation method of a three-dimensional fluffy porous scaffold

A porous scaffold and fluffy technology, which is applied in the field of preparation of three-dimensional fluffy porous scaffolds, can solve the problems of low porosity and poor connectivity of porous scaffolds, and achieve the effect of ensuring safety, non-toxicity, high porosity and high porosity

Active Publication Date: 2021-11-09
SHINCELL NEW MATERIAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is to provide a method for preparing a three-dimensional fluffy porous scaffold for the disadvantages of low porosity and poor connectivity in the prior art.

Method used

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  • A preparation method of a three-dimensional fluffy porous scaffold
  • A preparation method of a three-dimensional fluffy porous scaffold
  • A preparation method of a three-dimensional fluffy porous scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] S1. Polycaprolactone and 2,2,2-tetrafluoroethanol are dissolved to obtain a polymer solution with a concentration of 10wt.%;

[0036] S2. The polycaprolactone solution prepared in step S1 is subjected to electrospinning with a voltage of 18kV and a receiving distance of 15cm, and a polycaprolactone nanofiber is obtained by using alcohol with a volume concentration of 70% as the electrospinning receiving solution;

[0037] S3. place the polycaprolactone nanofibers prepared in step S2 in a pre-cooled alcohol / dry ice mixture with a mass ratio of 1:3, and let stand for 25min;

[0038] S4. Take out the polycaprolactone nanofibers processed in step S3 and place them in water at 25° C. for 5 minutes, and then take them out and quickly freeze them with liquid nitrogen for 15 minutes;

[0039] S5. freeze-dry the polycaprolactone nanofibers frozen in step S4 for 24 hours to obtain a polycaprolactone three-dimensional porous scaffold.

Embodiment 2

[0041] S1. Polycaprolactone and 2,2,2-tetrafluoroethanol are dissolved to obtain a polymer solution with a concentration of 8wt.%;

[0042] S2. carrying out the electrospinning of the polycaprolactone solution prepared in step S1 with a voltage of 15kV and a receiving distance of 10cm, and using alcohol with a volume concentration of 70% as the electrospinning receiving solution to obtain polycaprolactone nanofibers;

[0043] S3. place the polycaprolactone nanofibers prepared in step S2 in a pre-cooled alcohol / dry ice mixture with a mass ratio of 1:3, and let stand for 20min;

[0044] S4. Take out the polycaprolactone nanofibers treated in step S3 and place them in water at 20° C. for 7 minutes, then take them out and quickly freeze them with liquid nitrogen for 10 minutes;

[0045] S5. freeze-drying the polycaprolactone nanofibers frozen in step S4 for 36 hours to obtain a polycaprolactone three-dimensional porous scaffold.

Embodiment 3

[0047] S1. Polycaprolactone and 2,2,2-tetrafluoroethanol are dissolved to obtain a polymer solution with a concentration of 15wt.%;

[0048] S2. The polycaprolactone solution prepared in step S1 is subjected to electrospinning with a voltage of 20 kV and a receiving distance of 20 cm, and a polycaprolactone nanofiber is obtained by using alcohol with a volume concentration of 70% as the electrospinning receiving solution;

[0049] S3. Place the polycaprolactone nanofibers prepared in step S2 in a pre-cooled alcohol / dry ice mixture with a mass ratio of 1:5, and let stand for 30min;

[0050] S4. Take out the polycaprolactone nanofibers processed in step S3 and place them in water at 30°C for 3 minutes, then take them out and freeze them quickly with liquid nitrogen for 20 minutes;

[0051] S5. freeze-dry the polycaprolactone nanofibers frozen in step S4 for 12 hours to obtain a polycaprolactone three-dimensional porous scaffold.

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Abstract

The invention discloses a preparation method of a three-dimensional fluffy porous support. The invention uses biocompatibility, green and degradable polymers as raw materials, and prepares nanofiber structures through electrospinning. The nanofibers are placed in alcohol / dry ice mixed liquid, and the sublimation of dry ice is used to leave behind the nanofibers inside the nanofibers. Porous structure, the porous structure was quickly frozen in liquid nitrogen, and the three-dimensional fluffy porous scaffold was obtained after freeze-drying. The method of the invention is simple and easy, and the prepared three-dimensional fluffy porous scaffold has large pore diameter, high porosity and good connectivity between cells, which can well promote the growth of cells inside the scaffold.

Description

technical field [0001] The present invention relates to the technical field of porous materials, and more particularly, to a preparation method of a three-dimensional fluffy porous scaffold. Background technique [0002] In tissue engineering, a good bioscaffold should have rigidity and flexibility, appropriate degradation rate and good biocompatibility, and simulate the internal environment of cells in vivo as much as possible, so as to provide cells with the microenvironment required for growth. Nanomaterials have been more and more widely used in the field of tissue engineering due to their good simulation of the topology of cells in vivo. Electrospinning technology can continuously prepare nano-scale or sub-micron-scale ultrafine fibers, and the prepared scaffolds have unique microstructure and appropriate mechanical properties, which can simulate the nano-network structure of natural extracellular matrix. has unique advantages. [0003] However, traditional electrospi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D04H1/728D04H1/4326D04H1/4358D04H1/435D06C7/00A61L27/56D01F6/62
CPCA61L27/56A61L2400/12D01F6/625D04H1/4326D04H1/435D04H1/4358D04H1/728D06C7/00
Inventor 经鑫米皓阳刘跃军
Owner SHINCELL NEW MATERIAL CO LTD
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