Making method of microbial fermentation feed capable of increasing meat quality of pork
A technology of microbial fermentation and production method, applied in the field of microbial fermentation feed production, can solve problems such as poor controllability and optimization of mutual relations, and achieve the effects of high production efficiency, reduced equipment investment, and shortened fermentation cycle.
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Embodiment 1
[0026] A kind of microbial fermented feed preparation method that can improve pork meat quality
[0027] (1) Cultivate the original strains of Bacillus subtilis, Bacillus licheniformis, Lactobacillus acidophilus, Lactobacillus lactis, Enterococcus faecalis, Saccharomyces cerevisiae and Candida utilis at pH=8, 6, 4, 2 respectively After each culture, the strains with the best growth were selected and cultured on a medium with a lower pH, and finally the acid-resistant strains were selected.
[0028] (2) Place the bacteria solution of the acid-resistant and acclimated strain in a constant temperature water bath at 45°C for 60 minutes, then inoculate the bacteria solution on a solid plate for cultivation, then inoculate the culture on the solid plate in a liquid medium for cultivation, and finally A strain resistant to acid and heat is obtained.
[0029] (3) The temperature-resistant bacterial strains are cultivated successively on the medium of cholic acid concentration=5%, 10%...
Embodiment 2
[0034]A kind of microbial fermented feed preparation method that can improve pork meat quality
[0035] (1) Cultivate the original strains of Bacillus subtilis, Bacillus licheniformis, Lactobacillus acidophilus, Lactobacillus lactis, Enterococcus faecalis, Saccharomyces cerevisiae and Candida utilis at pH=8, 6, 4, 2 respectively After each culture, the strains with the best growth were selected and cultured on a medium with a lower pH, and finally the acid-resistant strains were selected.
[0036] (2) Place the bacteria solution of the acid-resistant and acclimated strain in a constant temperature water bath at 45°C for 60 minutes, then inoculate the bacteria solution on a solid plate for cultivation, then inoculate the culture on the solid plate in a liquid medium for cultivation, and finally A strain resistant to acid and heat is obtained.
[0037] (3) The temperature-resistant bacterial strains are cultivated successively on the medium of cholic acid concentration=5%, 10%,...
Embodiment 3
[0042] A kind of microbial fermented feed preparation method that can improve pork meat quality
[0043] (1) Cultivate the original strains of Bacillus subtilis, Bacillus licheniformis, Lactobacillus acidophilus, Lactobacillus lactis, Enterococcus faecalis, Saccharomyces cerevisiae and Candida utilis at pH=8, 6, 4, 2 respectively After each culture, the strains with the best growth were selected and cultured on a medium with a lower pH, and finally the acid-resistant strains were selected.
[0044] (2) Place the bacteria solution of the acid-resistant and acclimated strain in a constant temperature water bath at 45°C for 60 minutes, then inoculate the bacteria solution on a solid plate for cultivation, then inoculate the culture on the solid plate in a liquid medium for cultivation, and finally A strain resistant to acid and heat is obtained.
[0045] (3) The temperature-resistant bacterial strains are cultivated successively on the medium of cholic acid concentration=5%, 10%...
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