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Preparation method of deer blood peptide liposome

A liposome and deer blood peptide technology, applied in the field of deer blood, can solve the problems of restricting the consumption of deer blood, achieve good application prospects, improve bioavailability, and expand the application range

Inactive Publication Date: 2017-04-05
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In ancient my country, as early as the Song Dynasty, the nobles of the palace raised deer blood for raw drinks. However, this way of eating limited the number of people who can eat deer blood. It is urgent to develop deer blood health products that are convenient to eat and have clear effects, so that deer blood, a royal nourishment Products into the families of ordinary people

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Using fresh deer blood as raw material, deer blood peptides are prepared according to the following process:

[0019] Take 1 liter of fresh deer blood, centrifuge at 4°C and 3000 rpm for 1 hour, take the supernatant and denature it at 100°C for 1 hour, add 1 liter of pH 2 Tris-HCl buffer solution to the deer blood plasma protein after denaturation, Add pepsin, the amount of enzyme added is enzyme: protein (plasma protein) mass ratio = 1:100, hydrolyze at 20°C for 0.5 hours, freeze-dry after the reaction, and obtain powdered deer blood active peptide.

[0020] Take 50mg of dry phospholipid and cholesterol mixture, the mass ratio of phospholipid to cholesterol is 1:0.1, dissolve the mixture in 50ml of chloroform and mix well, then vacuum rotary evaporation at 80℃ to form a lipid film. Hydrate the lipid film with 500ml of deionized water, add glass beads with a diameter of 0.5-3mm, stir at 90°C for 60 minutes, and then stir at room temperature for 60 minutes to form liposo...

Embodiment 2

[0022] Take 1 liter of fresh deer blood, centrifuge at 4°C and 15,000 rpm for 0.1 hour, take the supernatant and add 480 grams of urea, denature it for 1 hour, add 25 liters of pH 8 phosphate buffer, add neutral protease, enzyme The amount added is enzyme: protein = 1:500, hydrolyzed at 80°C for 24 hours, and freeze-dried after the reaction to obtain powdered deer blood active peptide.

[0023] Take 50mg of dry phospholipid and cholesterol mixture, the mass ratio of phospholipid and cholesterol is 1:0.01, dissolve the mixture in 1000ml of chloroform and mix well, then evaporate in vacuum at 40℃ to form a layer of lipid film. Add 500ml of deionized water to hydrate the lipid film, add glass beads with a diameter of 0.5-3mm, stir at 90°C for 60 minutes, and then stir at room temperature for 60 minutes to form liposomes. The above-prepared buckblood peptide was dissolved in pH 7.4 phosphate buffer to prepare a buckblood peptide solution with a concentration of 5 mg / ml; 50 ml of b...

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Abstract

The invention relates to a deer blood peptide liposome and especially relates to a preparation method of the deer blood peptide liposome. The deer blood peptide liposome is prepared from a deer blood peptide and has functions of keeping deer blood peptide activity and resisting the degradation of digestive enzymes. The deer blood peptide can enter a blood system and can produce effects at a target organ. The deer blood peptide liposome solves the problem that the oral deer blood peptide can be easily degraded through the digestive enzymes and loses activity, improves bioavailability of oral deer blood peptide, enlarges a deer blood peptide eating method range, can be used in the fields of drugs, functional foods and food additives and has a wide application prospect.

Description

technical field [0001] The invention relates to deer blood, in particular to a deer blood peptide liposome and a preparation method thereof. Background technique [0002] Deer blood has been a precious tonic since ancient times. Chinese herbal books such as "Compendium of Materia Medica" and "New Edition of Materia Medica" have records of the medicinal value of deer blood. The use of deer blood for fitness was first seen in the "Qingbo Magazine" in the Song Dynasty. The "Compendium of Materia Medica" written by Li Shizhen in the Ming Dynasty recorded the medical effects of deer blood in detail: "mainly impotence, tonic, lumbago, falls, rabies, and Take alcohol to control lung impotence, hematemesis, and collapse, and those who are suffering from qi pain and want to be in danger drink it to heal immediately. It can greatly tonify deficiency and damage, benefit essence and blood, relieve acne poison, and drug poison. In ancient my country, as early as the Song Dynasty, the roya...

Claims

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Application Information

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IPC IPC(8): A61K38/01A61K9/127C12P21/06A23L33/18A61P9/12A61P39/06A61P37/04A61P35/00
Inventor 邹汉法靳艳
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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