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In-vitro rapid propagation method for haworthia cooperi v. truncata

A fast, in vitro technology, applied in Ji Yulu's field of in vitro rapid propagation, can solve the problems of expensive plants, large impact on the natural environment, long cycle, etc., and achieve the effect of improving the reproduction efficiency and the method is simple and easy to implement

Inactive Publication Date: 2017-04-26
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the extremely slow growth of Ji Yulu itself and strict environmental conditions, the maintenance cost is high, the plants are expensive, and the current research on succulents is relatively lagging behind.
At present, Ji Yulu's reproduction mainly relies on branch reproduction. Although seed reproduction is also one of the commonly used methods of reproduction, it must be artificially pollinated to obtain seeds.
Moreover, these conventional breeding methods have a long cycle and are greatly affected by the natural environment.
It is difficult to meet the demand for Ji Yulu in the market
In order to overcome the above problems, adopt tissue culture technology to speed up the reproduction and growth of Ji Yulu and reduce the cost, then the ornamental value of Ji Yulu will be fully displayed, and people can also feel the joy and health and environmental protection it brings. There is no report about Ji Yulu's in vitro rapid propagation method

Method used

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  • In-vitro rapid propagation method for haworthia cooperi v. truncata

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] When the present invention is concretely implemented, comprise the following steps:

[0015] 1) Sterilize the surface of explants: use the leaves of Ji Yulu as explants, dissolve 0.05g of soap in 150ml of tap water to make soapy water, then soak the leaves of Ji Yulu in soapy water for 15 minutes, and put them under running water Rinse for 2 hours, put the rinsed leaves of Ji Yulu on the sterile operating table, disinfect the surface with alcohol with a volume concentration of 75% for 30 seconds, and then use HgCl with a mass concentration of 0.1% 2 Surface disinfection for 7 minutes, and finally rinse with sterile water 5 times;

[0016] 2) Induction of callus: cut the surface-sterilized leaf explants into 0.3 cm on a sterile operating table 2 Put the small pieces of the explants into the high-pressure sterilized medium, each bottle of medium is 50ml, put 5 pieces of explants in each bottle, and carry out the cultivation under the temperature of 23 ℃, the light intens...

Embodiment 2

[0022] When the present invention is concretely implemented, comprise the following steps:

[0023] 1) Sterilize the surface of explants: use the leaves of Ji Yulu as explants, dissolve 0.08g of soap in 180ml of tap water to make soapy water, then soak the leaves of Ji Yulu in soapy water for 15 minutes, and put them under running water Rinse for 2 hours, put the rinsed leaves of Ji Yulu on the sterile operating table, disinfect the surface with alcohol with a volume concentration of 75% for 30 seconds, and then use HgCl with a mass concentration of 0.1% 2 Surface disinfection for 7 minutes, and finally rinsed with sterile water 6 times;

[0024] 2) Induction of callus: the surface sterilized leaf explants were cut into 0.4 cm on a sterile operating table 2 Put the small pieces of the explants into the high-pressure sterilized medium, each bottle of medium is 50ml, put 5 pieces of explants in each bottle, and carry out in the cultivation room temperature 25 ℃, light intensity...

Embodiment 3

[0030] When the present invention is concretely implemented, comprise the following steps:

[0031] 1) Sterilize the surface of explants: use the leaves of Ji Yulu as explants, dissolve 0.1g of soap in 200ml of tap water to make soapy water, then soak the leaves of Ji Yulu in soapy water for 15 minutes, and put them under running water Rinse for 2 hours, put the rinsed leaves of Ji Yulu on the sterile operating table, disinfect the surface with alcohol with a volume concentration of 75% for 30 seconds, and then use HgCl with a mass concentration of 0.1% 2 Surface disinfection for 7 minutes, and finally rinsed with sterile water for 7 times;

[0032] 2) Induction of callus: the surface sterilized leaf explants were cut into 0.6 cm on a sterile operating table 2 Put the small pieces of the explants into the high-pressure sterilized medium, each bottle of medium is 50ml, put 5 pieces of explants in each bottle, and carry out the cultivation under the temperature of 27°C, the lig...

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Abstract

The invention relates to an in-vitro rapid propagation method for haworthia cooperi v. truncata, and the in-vitro rapid propagation method can effectively solves the problems that conventional haworthia cooperi v. truncata propagation method is relatively long in period, is affected greatly by a natural environment, and cannot meet needs, on haworthia cooperi v. truncata, of the market. According to the technical scheme for solving the problems, the in-vitro rapid propagation method comprises the following steps: 1) sterilizing an explants surface; 2) inducing a callus; 3) inducing buds; 4) performing bud proliferation; 5) rooting; and 6) acclimatizating and transplanting. The in-vitro rapid propagation method is simple and easy to implement, can increase propagation efficiency within a short time, provides early-stage technical support for industrialized production of the haworthia cooperi v. truncata, is not affected by a natural environment, can be used for large-scale rapid propagation of the haworthia cooperi v. truncata, provides certain reference for researching other succulent plants and is innovative on the haworthia cooperi v. truncata propagation method.

Description

technical field [0001] The invention relates to a method for plant tissue culture in biotechnology, in particular to a method for in vitro rapid propagation of Ji Yulu. Background technique [0002] Jiyulu (Haworthia cooperi v.truncata) flower is as its name suggests, small, transparent, and elegant in color. It is a very cute succulent plant in the genus Twelve Volumes of Liliaceae. It is not only pleasing to the eye, And can purify the air. In recent decades, Ji Yulu has become a succulent plant that people are vying to collect and maintain because of its strong ornamental value, tenacious vitality, and the development of the Internet. ". However, because Ji Yulu itself grows extremely slowly and requires strict environmental conditions, the maintenance cost is high, the plants are expensive, and the current research on succulents is relatively lagging behind. At present, Ji Yulu's reproduction mainly relies on branch propagation. Although seed propagation is also one o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 董诚明苏秀红王琳琳魏硕熊玉平
Owner HENAN UNIV OF CHINESE MEDICINE
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