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Selenium modification method for improving immunological competence of radix pseudostellariae polysaccharides

The technology of immunological activity and modification method is applied in the field of selenization modification for improving the immunological activity of Polysaccharide Polysaccharide, which can solve the problems of barium chloride residue, polysaccharide efflux, low extraction rate of selenized polysaccharide, etc., so as to improve the yield and ensure the purity. Effect

Inactive Publication Date: 2017-04-26
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0004] The current method of selenized polysaccharides includes the nitric acid-sodium selenite method, and there are three problems in this method: 1. some polysaccharides will flow out during dialysis with a dialysis bag, resulting in low extraction rate of selenized polysaccharides
2. Although the use of barium chloride as a catalyst can improve the preparation speed of selenized polysaccharides, it will cause the residue of barium chloride at the same time, making the selenized polysaccharides impure
3. To test the success of selenization modification, hydride generation-atomic fluorescence spectroscopy and infrared spectroscopy are generally used, but the molecular weight before and after selenization is not clear

Method used

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  • Selenium modification method for improving immunological competence of radix pseudostellariae polysaccharides
  • Selenium modification method for improving immunological competence of radix pseudostellariae polysaccharides

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Embodiment 1

[0018] A selenization modification method for improving the immune activity of polysaccharides from Radix Pseudostellariae, said method comprising the following steps:

[0019] (1) Take 9 parts of 500 mg polysaccharides of Pseudostellaria Radix Pseudostellariae, put them in 50 mL of water, dissolve them under magnetic stirring, put them into three-neck flasks respectively, slowly add 2 mL of glacial acetic acid and 0.6 g of magnesium ferrite dropwise; add Na 2 SeO 3 200-400mg, react at 50-90℃ for 6-10h;

[0020] (2) Cool to room temperature after the reaction, adjust the pH value to 6 with anhydrous sodium carbonate, centrifuge, filter, and put the solution into a 1000 D dialysis bag for dialysis; take a sample of the dialysate every 6 hours, and use the ascorbic acid method to detect whether it contains sub- Sodium selenate, stop dialysis when there is no sodium selenite residue or no red color;

[0021] (3) The solution was subjected to Sephadex G-100 column chromatography...

Embodiment 2

[0025] The mice had free access to food and water, the temperature was controlled at 22±1°C, and the light was cycled for 12 h. Mice were randomly divided into 11 groups, including healthy control group, drug treatment group, and cyclophosphamide (CY) model group, 20 in each group, and all animals were adaptively fed for 7 days. The healthy control group and the cyclophosphamide (CY) model group were subcutaneously injected with normal saline for 14 days. Administration group sRPP 2 , sRPP 3 , RPP was divided into three groups of three concentrations (1 mg / mL, 2 mg / mL, 3 mg / mL), a total of 9 groups, subcutaneous injection of 0.2 mL, continuous administration for 14 days. From the 15th day, except the healthy control group, 9 administration groups and cyclophosphamide (CY) model group received intraperitoneal injection of cyclophosphamide, 80 mg / kg·d, once a day, for 3 consecutive days.

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Abstract

The invention provides a selenium modification method for improving the immunological competence of radix pseudostellariae polysaccharides. The selenium modification method comprises the following steps: taking the radix pseudostellariae polysaccharides, reacting with Na2SeO3 under certain conditions, cooling to a room temperature after the end of reaction, adjusting a pH value to 5-6 with anhydrous sodium carbonate, centrifuging, filtering and putting a solution into a 1000-D dialysis bag for flowing water dialysis; taking a dialysate sample once every 6 hours, detecting whether the dialysate sample contains sodium selenite or not by using an ascorbic acid method, and stopping dialysis when no sodium selenite residue exists or the red is not displayed; and performing Sephadex G-100 column chromatography on the solution to obtain the radix pseudostellariae polysaccharides. When nitric acid is used for reaction, the cleavage of a polysaccharide chain can be caused, so that oligosaccharides are formed, and the low yield of selenium-containing polysaccharides is caused. By using glacial acetic acid, the cleavage of the polysaccharide chain is difficultly caused, so that the yield of selenium-containing polysaccharides is improved.

Description

technical field [0001] The invention belongs to the field of veterinary medicine, and in particular relates to a selenization modification method for improving the immune activity of heterophylla polysaccharides. Background technique [0002] Pseudostellaria Pseudostellariae is a commonly used traditional Chinese medicine recorded in the "Chinese Pharmacopoeia". It has the effects of nourishing qi and invigorating the spleen, promoting body fluid and moisturizing the lungs. It is clinically used for symptoms such as spleen deficiency, fatigue, loss of appetite, and weakness after illness. Pharmacological tests have shown that Pseudostellaria polysaccharides can enhance the immune function of the body, and Pseudostellaria polysaccharides are neutral glucans. [0003] Selenium is an essential element for life activities, and plays an important role in human cancer prevention and anti-cancer, anti-aging, improving body immunity, preventing and treating cardiovascular diseases a...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P37/02
CPCC08B37/00
Inventor 秦韬黄一帆李健马玉芳黄小红俞道进任喆
Owner FUJIAN AGRI & FORESTRY UNIV
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