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Method for disinfection and anti-stress treatment of leech seedlings

A leech, anti-stress technology, applied in applications, anti-toxic agents, pharmaceutical formulations, etc., can solve problems such as excessive drug residues, pathogen resistance (resistance), and irritating odors, so as to improve the economic benefits of breeding and escape everywhere. The effect of reducing the rate and slowing down the stress response

Inactive Publication Date: 2020-10-16
GUANGXI UNIV OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] Although Hirudo philippines has formed a certain scale of artificial breeding, there are still many imperfections.
In the disinfection of Hirudo phenatus seedlings, although it has been reported that 4-8ppm bleaching powder or 1.3-2.7ppm strong chlorine or 0.009mL / L chlorine preparations or 0.003mL / L povidone can be used Iodine preparations or 0.002mL / L iodine preparations, but these disinfectants are all chemicals, and some even have a strong pungent smell, which all produce certain toxic and side effects
In addition, the use of chemicals will not only lead to drug resistance (resistance) of pathogenic bacteria, but also lead to problems such as exceeding the standard of drug residues, which seriously affects the effect of Hirudo phenanthae as a traditional Chinese medicine

Method used

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  • Method for disinfection and anti-stress treatment of leech seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Breeding Hirudo phenatus in cement ponds for seedling disinfection and anti-stress treatment.

[0051] 1. Cultivate Hirudo phenanthae in a cement pond with an area of ​​15 square meters for seedling disinfection and anti-stress treatment;

[0052] 2. About 50 catties of seedlings of B. philippines have been tied into 100-mesh mesh bags. Put the leech phenanthrene seedlings in a dry place in the farm, and place the mesh bag horizontally for 10 minutes;

[0053] 3. During this waiting process, configure 10ml of Chinese herbal medicine reagent mainly composed of rhubarb essence, dilute it with water (it is sufficient to allow the leeches to be completely soaked in water, and the concentration range can be controlled at 0.3ml / cubic water) and then put in in a plastic box; then completely soak the seedlings in a plastic box with Chinese herbal medicine reagents for 15 minutes;

[0054] 4. After soaking, place the seedlings of B. phenanthae on the spawning platfor...

Embodiment 2

[0056] Example 2 The seedling disinfection and anti-stress treatment of Hirudo phenanthae were cultured in net cages.

[0057] 1. In an area of ​​50 square meters of pond cages to breed Hirudo phenanthae, carry out seedling disinfection and anti-stress treatment;

[0058] 2. About 100 kilograms of Hirudo spp. seedlings have been tied in a 110-mesh mesh bag. Put the Hirudo philippines seedlings in a dry place by the pond, and place the mesh bags horizontally for 15 minutes;

[0059] 3. During this waiting process, configure 20ml of Chinese herbal medicine reagent mainly composed of rhubarb essence, dilute it with water (it is sufficient to allow the leeches to be completely soaked in water, and the concentration range can be controlled at 0.4ml / cubic water) and then put in In a plastic box; completely soak the seedlings in a plastic box with Chinese herbal medicine reagents for 20 minutes;

[0060] 4. After soaking, put the seedlings of B. phenanthae on the spawning platform i...

Embodiment 3

[0062] Example 3 Cultivation of Hirudo phenatus in cement ponds for disinfection of seedlings and anti-stress treatment.

[0063] 1. Cultivate Hirudo phenanthae in a cement pond with an area of ​​15 square meters for seedling disinfection and anti-stress treatment;

[0064] 2. About 50 catties of Hirudo phenanthae seedlings have been tied in a 90-mesh mesh bag. Put the Hirudo phenanthae seedlings in a dry place in the farm and place the mesh bag horizontally for 10 minutes. A layer of black canvas is placed on the top;

[0065] 3. During this waiting process, configure 10ml of Chinese herbal medicine disinfectant solution mainly composed of rhubarb essence, dilute it with water (you can completely soak the leeches in the water, and the concentration range can be controlled at 0.3ml / cubic water) and then put it put in a plastic box;

[0066] 4. Then completely soak the seedlings in a plastic box with Chinese herbal medicine reagents for 15 minutes; wherein, in parts by weight,...

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Abstract

The invention discloses a hirudinaria manillensis lesson seedling disinfection and anti-stress processing method, which comprises the following steps: filling hirudinaria manillensis lesson seedlings into a mesh bag and tightening with a rope; horizontally placing the mesh bag on the dry ground of a farm for 10-15min; immersing the mesh bag together with hirudinaria manillensis lesson into a disinfection solution and continuously soaking for 15-20min; placing the mesh bag with soaked and disinfected hirudinaria manillensis lesson in the middle of or around a cultivating pool of the farm, then untightening the mesh bag of the hirudinaria manillensis lesson seedlings so as to lead the hirudinaria manillensis lesson seedlings to self crawl into the cultivating pool; and after the hirudinaria manillensis lesson seedlings enter the cultivating pool, immediately splashing an anti-stress solution evenly into the whole cultivating pool. By utilizing the method, the safe, effective, pollution-free, green and environment-friendly traditional Chinese herbal disinfection and anti-stress processing can be conducted to the hirudinaria manillensis lesson seedlings, the disease of hirudinaria manillensis lesson seedlings can be effectively prevented, the death rate of the hirudinaria manillensis lesson seedlings can be reduced, so that the survival rate of the seedlings can be improved, and the cultivation benefits can be greatly increased.

Description

technical field [0001] The invention relates to the technical field of A. phenanthae breeding, in particular to a method for disinfection and anti-stress treatment of A. phenanthae seedlings. Background technique [0002] Hirudinaria manillensis Lesson (1842), commonly known as "Phnom Penh leech", belongs to the genus Hirudinaria genus Hirudinaria and is mainly distributed in Guangxi, Guangdong, Hainan and other provinces in my country and the tropical regions of Southeast Asia. It is more common in paddy fields, ditches or ponds, and lives by sucking the blood of vertebrates such as humans, livestock, and frogs in a natural environment. Hirudin salivary gland secretions and body are rich in hirudin, which has special curative effect in treating cardiovascular and cerebrovascular diseases such as thrombosis, high blood pressure, and high blood fat. Hirudo philippines is a characteristic medicinal animal resource in Guangxi. As early as 2011, Hirudocarpus was included in the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/40A61L2/18A61K36/9068A61K38/05A61P39/00A61K31/375A61K31/216A61K35/62A61K31/355A61K31/51A61K31/525A61K31/715A61K31/722A61K36/254A61K31/353A61K31/7048A61K31/122
CPCA01K61/00A61K31/122A61K31/216A61K31/353A61K31/355A61K31/375A61K31/51A61K31/525A61K31/7048A61K31/715A61K33/30A61K35/62A61K36/254A61K36/9068A61K38/05A61L2/0088A61K2300/00
Inventor 黄勇缪剑华张月云韦范韦桂丽
Owner GUANGXI UNIV OF CHINESE MEDICINE
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