A kit for detecting fibrinogen content

A fibrinogen and kit technology, applied in the biological field, can solve problems such as unmentioned significance, and achieve the effects of high accuracy, good stability and wide detection range

Active Publication Date: 2018-08-03
BEIJING SICCEEDER TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, few manufacturers in the market have mentioned "calcium-containing" reagent systems, and most products ignore or do not mention Ca in reagents. 2+ Significance of Quantitative Detection of FIB in Plasma

Method used

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  • A kit for detecting fibrinogen content
  • A kit for detecting fibrinogen content
  • A kit for detecting fibrinogen content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 Preparation and use method of kit of the present invention

[0047] 1. Kit preparation

[0048] (1) Thrombin reagent (pH 7.4):

[0049]

[0050] (2) Imidazole buffer solution (pH value is 7.4):

[0051]

[0052] (3) Fixed value plasma: 3g / L.

[0053] 2. How to use the kit

[0054] The test kit of the present invention is carried out on the coagulation method full-automatic blood coagulation tester for routine inspection in hospital clinical laboratories, and its specific use method is:

[0055] 1. Preparation of samples and reagents:

[0056] (1) Prepare the plasma sample to be tested: blood is collected from a vein, and 109mmol / L sodium citrate is mixed with whole blood at a ratio of 1:9. Centrifuge at 2500g for 15 minutes and remove the plasma with a plastic pipette. Plasma samples must be tested within 4 hours.

[0057] (2) FIB thrombin reagent: each bottle of thrombin is accurately weighed according to the label on the bottle and added to dist...

Embodiment 2

[0069] Embodiment 2 The verification experiment of the stability of the optimal buffer system of the invention kit:

[0070] In order to find the optimal ratio of the main components in the reagent system, this experiment compared and analyzed the concentrations of the thrombin reagent and imidazole buffer components in the kit, and tested the preparations of the three buffer systems A, B, and C respectively. The FIB content changes of the reagents at 37°C were used to evaluate the stability of the corresponding reagent system.

[0071] Wherein, A, B, C reagent formula are as shown in Table 2:

[0072] Table 2 Reagent system composition with different concentrations:

[0073]

[0074] The specific experimental data are shown in Table 3 below:

[0075] Table 3 Stability of reagent systems with different concentrations

[0076]

[0077]

[0078] The above experimental data shows that the reagent kit adopts the B reagent system, which has better stability, and the dev...

Embodiment 3

[0079] Ca in embodiment 3 invention kit 2+ Verification experiments on the quantitative accuracy of FIB:

[0080] This experiment grouped as adding Ca to the thrombin reagent 2+ Ca-free in groups and thrombin reagents 2+ group, add Ca 2+ Group adopts the B reagent system of embodiment 2 to detect, without Ca 2+ Group adopts the reagent that lacks calcium chloride on the basis of the B reagent system of embodiment 2.

[0081] Each group randomly selects 10 reagents for quantitative detection of FIB. The reference value of control plasma FIB is 2.8g / L. 2+ The impact on FIB quantification, the relevant experimental data are shown in Table 4 below:

[0082] Table 4 Ca in thrombin reagent 2+ Effect on FIB Quantitative Accuracy

[0083]

[0084]

[0085] The above data show that the addition of Ca to the thrombin reagent 2+ The FIB detection value of the group is more consistent with the reference value, and the deviation is smaller, that is, adding Ca to the thrombin ...

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Abstract

The invention relates to the technical field of biology and particularly relates to a kit for detecting the content of fibrinogen. The kit comprises a thrombin reagent, an imidazole buffer solution and constant-value plasma, wherein the thrombin reagent comprises thrombin, imidazole, calcium chloride, mannitol, peptone, bovine serum albumin and sodium azide. A proper preparation scheme of the thrombin reagent and the imidazole buffer solution is determined and an in vitro diagnosis kit for clinical detecting the content of the FIB of human plasma in a hospital is researched. The thrombin reagent particularly contains Ca<2+> to reduce or supplement inhibited calcium ions in a clinical blood sample, an in vivo normal coagulation process is more ideally simulated and a more accurate data reference is provided for clinical detection. The basic performance of the kit reaches the industry standard requirements; the kit is high in accuracy, wide in detection range and good in stability and can be further popularized and applied to the market.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a detection kit for measuring fibrinogen content by a Clauss method. Background technique [0002] Fibrinogen is a glycoprotein synthesized by the liver with blood coagulation function and is the precursor of fibrin. Increased fibrinogen levels are more common in diabetes, inflammatory stress response, and obesity; lower fibrinogen levels are more common in DIC and fibrinolysis. Severe impairment of liver function or congenital deficiency can reduce the concentration of plasma fibrinogen, and in severe cases, there may be a bleeding tendency. In addition, fibrinogen has also been used in studies of the pathogenicity of cardiovascular accidents. [0003] Fibrinogen is synthesized in the liver (1.7-5g / day) and megakaryocytes, and the reference value in plasma is 2-4g / l. Fibrinogen is composed of six chains, 2Aα, 2Bβ, and 2γ. Under the action of thrombin (factor IIa), the fibrinogen ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/68G01N2333/75G01N2800/085G01N2800/32
Inventor 丁重辉闫君
Owner BEIJING SICCEEDER TECH CO LTD
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