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Extraction Technology of Corn Yellow Powder Protein and Its Application in Xanthan Gum Fermentation

A technology of corn yellow powder and extraction process, which is applied in the direction of plant zein, fermentation, and peptide preparation methods, can solve the problems of limited properties, difficult extraction process, and low content of corn glutenin research, and achieve waste reduction. For treasure, improve economic benefits, high yield and high purity effect

Active Publication Date: 2020-05-22
SHANDONG FUFENG FERMENTATION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on gluten is mainly concentrated on glutenin, and some people have studied the optimal extraction process of glutenin in rapeseed pollen, and the research on corn glutenin is only limited to the study of its properties
Albumin and corn polypeptide substances have high nutritional content, but the content is low, the extraction process is very difficult, and they are generally ignored or discarded, resulting in waste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The extraction process of corn gluten protein, it comprises the steps:

[0020] 1) Remove grease: take corn yellow powder, crush it, soak it in water five times its weight, stir at 500rpm for 10 minutes, then let it stand for 30 minutes, remove the upper layer of grease, and then centrifuge to collect the sediment and upper liquid;

[0021] 2) Preparation of fermentation medium: take glucose 9%, upper liquid 80%, magnesium sulfate heptahydrate 0.3%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.1%, ferrous sulfate heptahydrate 0.001%, VB according to weight percentage 1 0.00001%, the rest is water, stir evenly, adjust the pH to 7.2;

[0022] 3) Extrusion treatment: Add the precipitate obtained in step 1) to an extruder for extrusion treatment to obtain material A. The extrusion parameters are: the diameter of the die hole is 2mm, the temperature of the extruded material is 120°C, and the water content is 18wt%;

[0023] 4) Alcohol dissolution ...

Embodiment 2

[0027] A kind of technology that utilizes corn yellow powder extract fermentation to prepare xanthan gum, it comprises the steps:

[0028] The fermentation medium adopts the fermentation medium or the control medium prepared in Example 1 of the present invention respectively; wherein, the components of the control medium are: 9% of glucose, 0.8% of soybean protein, 2.5% of corn steep liquor, and sulfuric acid heptahydrate according to the percentage by weight. Magnesium 0.3%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.1%, ferrous sulfate heptahydrate 0.001%, VB 1 0.00001%, the rest is water, stir evenly, adjust pH to 7.2;

[0029] The fermentation step can adopt the following process: insert the Xanthomonas gummosa seed liquid into the fermentation medium according to the inoculum size of 5% (volume ratio) for cultivation, the temperature is 30° C., the pH value is 7.2, and the cultivation time is 60 hours to obtain the fermentation liquid After te...

Embodiment 3

[0032] Step) is not used to extrude, and all the other are the same as in Example 1.

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PUM

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Abstract

The invention belongs to the technical field of corn gluten meal deep processing, and discloses a corn gluten meal extraction process which includes the steps: 1) removing grease; 2) preparing a fermentation culture medium; 3) performing extrusion treatment; 4) performing alcohol-soluble and ultrasonic treatment; 5) extracting alcohol-soluble protein and yellow pigments; 6) extracting gluten. The fermentation culture medium is prepared while protein substances are extracted, and double benefits are achieved at one stroke.

Description

technical field [0001] The invention belongs to the technical field of corn yellow powder deep processing, and relates to an extraction process of corn yellow powder protein and its application in xanthan gum fermentation. Background technique [0002] Corn gluten meal (corn gluten meal) is a by-product of wet production of corn starch, containing about 60% protein, of which zein accounts for about 65% of protein, followed by pigments, oils, etc. Most of our country produces starch Enterprises sell corn yellow powder as cheap feed, and some companies do not make any use of corn yellow powder when producing starch, and discharge it with waste liquid, resulting in a waste of resources. [0003] Glamins are the main protein in corn flour. Osborne invented a method for extracting zein from corn flour, a by-product of wet starch production. This method increases the yield of zein from 5% to 30%. Since then, through the unremitting efforts of scientists, the extraction process ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/425C07K14/415C07K1/14C07K1/30C09B61/00C12P19/06C12R1/01
CPCC07K14/415C07K14/425C09B61/00C12P19/06
Inventor 贾召鹏李德衡赵兰坤刘元涛李树标刘建阳
Owner SHANDONG FUFENG FERMENTATION CO LTD