A kind of preparation method of antibacterial protein peptide-nanoparticle complex

A protein peptide and nanoparticle technology, which is applied in the preparation method of peptides, antibacterial drugs, hydrolyzed protein components, etc., can solve problems such as behavioral differences, and achieve the effects of reducing toxicity, benefiting health, and improving solubility

Active Publication Date: 2020-11-13
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Nanomaterials have certain uniqueness. When the material scale is small to a certain extent, quantum mechanics must be used instead of traditional mechanics to describe its behavior. When the powder particle size is reduced from 10 microns to 10 nanometers, its particle size Although the change is 1000 times, when converted into volume, it will be 10 to the 9th power, so there will be obvious differences in the behavior of the two

Method used

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  • A kind of preparation method of antibacterial protein peptide-nanoparticle complex
  • A kind of preparation method of antibacterial protein peptide-nanoparticle complex
  • A kind of preparation method of antibacterial protein peptide-nanoparticle complex

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Embodiment 1

[0023] A preparation method of antibacterial protein peptide-nanoparticle complex, specific preparation steps:

[0024] 1) Preparation of zinc oxide colloid: Add zinc acetate to absolute ethanol, and ultrasonically aid in dissolution to obtain zinc acetate ethanol solution. The concentration of zinc acetate ethanol solution is 0.08-0.12mol / L. Ethanol evaporates. Stir, add sodium hydroxide ethanol solution dropwise to zinc acetate ethanol solution, the concentration of sodium hydroxide ethanol solution is 0.16~0.24mol / L, the volume ratio of sodium hydroxide ethanol solution: sodium hydroxide ethanol solution is 1:0.8~ 1.3, the temperature of the water bath is 75-90°C, shake and stir for 1.5-3 hours, hydrolyze the zinc acetate, sol, and gel to obtain a zinc oxide colloidal solution;

[0025] 2) Preparation of nano-zinc material: add n-hexane and ethylene glycol monomethyl ether to the colloidal solution, n-hexane: colloidal solution volume ratio is 1:0.6-1.5, ethylene glycol mo...

Embodiment 2

[0028] A method for preparing an antibacterial protein peptide-nanoparticle complex, the most preferred preparation steps:

[0029] 1) Preparation of zinc oxide colloid: add zinc acetate to absolute ethanol, and ultrasonically aid in dissolution to obtain zinc acetate ethanol solution, the concentration of zinc acetate ethanol solution is 0.1mol / L, and it is prepared in a closed container to prevent ethanol volatilization . Stir, add the sodium hydroxide ethanol solution dropwise to the zinc acetate ethanol solution, the concentration of the sodium hydroxide ethanol solution is 0.2mol / L, the volume ratio of the zinc acetate ethanol solution:sodium hydroxide ethanol solution is 1:1, and the water bath temperature is 80°C, shaking and stirring for 2 hours, zinc acetate hydrolysis, sol, gel to obtain zinc oxide colloidal solution;

[0030]2) Preparation of nano-zinc material: add n-hexane and ethylene glycol monomethyl ether to the colloidal solution, n-hexane: colloidal solutio...

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Abstract

The invention provides a preparation method of an anti-microbial protein peptide-nanoparticle complex, which comprises operation steps: 1), preparation of zinc oxide colloid; 2), preparation of a nano-zinc material; 3) preparation of protein peptide-nanoparticles. The method has the beneficial effects: nano-zinc oxide has a specific adsorption effect on anti-microbial Setipinna protein peptides, so as to play a role of purification; the Setipinna protein peptides are absorbed on nano-zinc oxide, keep relatively independent space structures and biological activities, and are exponentially increased in specific surface area, so as to have a very strong anti-microbial effect; the nanometer material has slight toxicity, and is coated with a layer of polypeptide on the outer surface to reduce the toxicity, so as to be good for body health; the preparation method is simple, the cost is low, and a prepared product has a good anti-microbial effect and does not generate resistance to drugs, so as to be green and environment-friendly, high in safety, and suitable for mass production.

Description

technical field [0001] The invention relates to the technical field of composite material preparation, in particular to a method for preparing an antibacterial protein peptide-nanoparticle complex. Background technique [0002] Nanoparticle materials, also known as ultrafine particle materials, are composed of nanoparticles. Nanoparticles are also called ultrafine particles, which generally refer to particles with a size between 1 and 100nm, which are in the transition zone between atomic clusters and macroscopic objects. From the usual point of view of micro and macro, such a system is not typical. The microscopic system is not a typical macroscopic system, but a typical mesoscopic system, which has surface effects, small size effects and macroscopic quantum tunneling effects. When people subdivide a macroscopic object into ultrafine particles (nanoscale), it will show many strange properties, namely its optical, thermal, electrical, magnetic, mechanical and chemical prope...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/04A61K9/14A61K38/01A61K47/02A61P31/04
CPCA61K9/143A61K38/012
Inventor 宋茹韦荣编
Owner ZHEJIANG OCEAN UNIV
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