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Antibacterial biological membrane and preparation method thereof

A biofilm and antimicrobial peptide technology, applied in the medical field, can solve the problems of unstable and easy-to-degrade antibacterial biofilms

Inactive Publication Date: 2017-05-31
SHENZHEN QIKANG MEDICAL DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] But at present, the existing antibacterial biofilms are generally prepared using chitosan materials that have antibacterial, anti-inflammatory and other medical effects, but chitosan is easy to degrade, so the obtained antibacterial biofilms are unstable and easy to degrade.

Method used

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  • Antibacterial biological membrane and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Get the concentrated solution of pectin obtained in the production of high methoxy pectin, make its pectin concentration 2%; mix the antimicrobial peptide with 95% alcohol to obtain the antimicrobial peptide solution, control the antimicrobial peptide concentration at 7%; The antimicrobial peptide solution and the pectin solution were mixed in a ratio of 3:1 by volume, and reacted at 12°C for 2h; The solution was washed, and then washed with 60% alcohol; the filter cake was dried at 60°C, crushed, and pectin modified with antimicrobial peptides.

[0023] Concentration is that the pectin modified by 3wt% antimicrobial peptide is dissolved in the concentration of 1wt% acetic acid solution, then add glycerol thereinto, the dosage of glycerol is 25% of the quality of the pectin modified by antimicrobial peptide, prepare antimicrobial peptide modified pectin solution. 10 wt% collagen was dissolved in distilled water, and glycerin was added in an amount of 25% of the mass of...

Embodiment 2

[0029] This embodiment is basically the same as Example 1, except that the pectin concentration in the pectin solution is 2%; the antimicrobial peptide is mixed with 95% alcohol to obtain the antimicrobial peptide solution, and the antimicrobial peptide concentration is controlled at 6%. After the reaction finishes, take out the precipitate, filter, and the filter cake is washed with 60% alcohol, then washed with 60% alcohol solution of pH2, and then washed with 60% alcohol; : 1 ratio mixed, 10 ℃ reaction 3h; Concentration is that the pectin modified by 3wt% antimicrobial peptide is dissolved in the acetic acid solution that concentration is 0.5wt%, then adds glycerol thereinto, and the dosage of glycerol is the pectin modified by antimicrobial peptide The quality of the glue is to prepare a pectin solution modified by antimicrobial peptide; the mass ratio of pectin modified by antimicrobial peptide to collagen in the film-forming solution is 1:0.3, and the pH of the film-formi...

Embodiment 3

[0031] The present embodiment is basically the same as Example 1, except that the concentration of pectin in the pectin solution is 5%; the antimicrobial peptide is mixed with 95% alcohol to obtain the antimicrobial peptide solution, and the concentration of the antimicrobial peptide is controlled at 1%. After the reaction finishes, the precipitate is taken out, filtered, and the filter cake is washed with 60% alcohol, then washed with 60% alcohol solution of pH5, and then washed with 60% alcohol; : 1 ratio mixing, 15 ℃ of reaction 1h; Concentration is that the pectin modified by 3wt% antimicrobial peptide is dissolved in the acetic acid solution that concentration is 2wt%, then adds glycerol wherein, the dosage of glycerol is the pectin modified by antimicrobial peptide 10% of the mass of the film-forming solution; the mass ratio of antimicrobial peptide-modified pectin to collagen in the film-forming solution is 1:0.8, and the pH of the film-forming solution is 6.5.

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Abstract

The invention discloses an antibacterial biological membrane and a preparation method thereof. The preparation method comprises the following steps of firstly, selecting antibacterial peptide amidation pectin, so as to obtain antibacterial peptide-modified pectin; then, dissolving the antibacterial peptide-modified pectin, obtaining an antibacterial peptide-modified pectin solution, mixing with a collagen solution, and homogenizing, so as to form a membrane forming solution; finally, preparing the membrane forming solution to form the antibacterial biological membrane. The antibacterial biological membrane has the characteristics that the pectin is amidated, and then is mixed with collagen to obtain the antibacterial biological membrane; the efficiency is high, and the spectrum is broad; the research prospect and practical value are broad.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to an antibacterial biofilm and a preparation method thereof. Background technique [0002] Antimicrobial peptide is an important component of the innate immune system of organisms, and it is a general term for a variety of basic polypeptide molecules with antibacterial activity. Antimicrobial peptides have high-efficiency and broad-spectrum antibacterial activity, and can quickly kill targets, mainly acting on Gram-negative bacteria, Gram-positive bacteria, fungi, parasites, etc. Antimicrobial peptides also have potential anti-tumor activity, and can accelerate the body's immune and wound healing processes, making it possible to become a new type of drug that can replace traditional antibiotics and anti-tumor drugs. [0003] But at present, the existing antibacterial biofilms are generally prepared using chitosan materials with antibacterial, anti-inflammatory and other medical e...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61K9/70A61K47/42A61P1/12A61P17/02A61P35/00A61K31/732
CPCA61K9/7015A61K31/732A61K38/16A61K47/42
Inventor 佘振安
Owner SHENZHEN QIKANG MEDICAL DEVICES
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