Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Yellow tang sulfated polysaccharide as well as preparation method and application thereof

A technology of Ascophyllum nodosum sulfate ester and polysaccharide, which is applied in the field of polysaccharides, can solve the problems of less research on Ascophyllum nodosum polysaccharide, and achieve the effect of large molecular weight, stable active structure and high purity

Active Publication Date: 2017-05-31
江门市方程健康生物科技有限公司
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on sulfated polysaccharides mainly focuses on the research on laminarin, and the research on ascophyllin is less
At the same time, research on sulfated polysaccharides is generally carried out on low molecular weight polysaccharides, and less research on large molecular weight sulfated polysaccharides is involved.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Yellow tang sulfated polysaccharide as well as preparation method and application thereof
  • Yellow tang sulfated polysaccharide as well as preparation method and application thereof
  • Yellow tang sulfated polysaccharide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] A kind of preparation method of Ascophyllum nodosum sulfated polysaccharide provided in this embodiment, it comprises:

[0056] The Ascophyllum nodosum sample was crushed through a 60-mesh sieve, soaked in 80% ethanol and dried in the air, and repeated several times to remove pigments, polyphenols, monosaccharides and oligosaccharides.

[0057] Extract the impurity-removed Ascophyllum nodosum in water at 60-100°C with a liquid-to-solid ratio of 20mL / g for 3 times, each time for 4 hours, centrifuge, combine the extracts for three times, and concentrate to obtain a water extract.

[0058] Add absolute ethanol to the water extract for alcohol precipitation, and control the ethanol concentration in the alcohol precipitation system to 70%, overnight at 4°C, centrifuge, and collect the precipitate to obtain the precipitate.

[0059] Redissolve the precipitate in pure water, put the sample into a dialysis bag with a cutoff of 8000-14000Da for dialysis for 5 days, then concentr...

Embodiment 2

[0063] A kind of preparation method of Ascophyllum nodosum sulfated polysaccharide provided in this embodiment, it comprises:

[0064] The Ascophyllum nodosum sample was crushed through a 60-mesh sieve, soaked in 70% ethanol and dried in the air, and repeated several times to remove pigments, polyphenols, monosaccharides and oligosaccharides.

[0065] Extract the impurity-removed Ascophyllum nodosum in water at 60-100°C with a liquid-to-solid ratio of 5mL / g for 4 times, each time for 5h, centrifuge, combine the four extracts, and concentrate to obtain the water extract.

[0066] Add absolute ethanol to the water extract for alcohol precipitation, and control the ethanol concentration in the alcohol precipitation system to 60%, overnight at 1°C, centrifuge, and collect the precipitate to obtain the precipitate.

[0067] The precipitate was redissolved in pure water, and the sample was put into a dialysis bag with a cutoff of 8000-14000Da for dialysis for 3 days, then concentrat...

Embodiment 3

[0071] A kind of preparation method of Ascophyllum nodosum sulfated polysaccharide provided in this embodiment, it comprises:

[0072] After the Ascophyllum nodosum sample was crushed through an 80-mesh sieve, it was soaked in 90% ethanol and dried in the air, and repeated several times to remove pigments, polyphenols, monosaccharides and oligosaccharides.

[0073] Extract the impurity-removed Ascophyllum nodosum in water at 60-100°C with a liquid-to-material ratio of 30mL / g, extract twice for 3 hours each time, combine the two extracts after centrifugation, and concentrate to obtain a water extract.

[0074] Add absolute ethanol to the water extract for alcohol precipitation, and control the ethanol concentration in the alcohol precipitation system to 80%, overnight at 8°C, centrifuge, and collect the precipitate to obtain the precipitate.

[0075] The precipitate was redissolved in pure water, and the sample was put into a dialysis bag with a cutoff of 8000-14000 Da for dial...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides yellow tang sulfated polysaccharide as well as a preparation method and application thereof, and relates to the technical field of polysaccharide. The preparation method of the yellow tang sulfated polysaccharide comprises the step of carrying out edulcoration, aqueous extraction, alcohol precipitation and dialysis on yellow tang by adopting alcohol or an aqueous solution of alcohol so as to obtain a polysaccharide crude product. A polysaccharide component with large molecular weight is purified and obtained by adopting an anion-exchange column, and a gel column is adopted for purifying the polysaccharide component with large molecular weight so as to obtain the yellow tang sulfated polysaccharide. The preparation method is high in raw material utilization rate, mild in technology and high in purity, and the stability of the active structure of the sulfated polysaccharide is sufficiently guaranteed. Poisonous and harmful reagents such as acid and alkali are not used in the preparation process, and the yellow tang sulfated polysaccharide is applicable to large-scale production. Moreover, the invention also relates to the yellow tang sulfated polysaccharide prepared by the preparation method and application thereof. The sulfated polysaccharide is large in molecular weight and high in acidophobe content, can adjust the level of intestinal flora, is applied to preparation of products for adjusting intestinal functions, and has great development and application prospects.

Description

technical field [0001] The invention relates to the field of polysaccharides, and in particular to a sulfated polysaccharide from Ascophyllum nodosum and its preparation method and application. Background technique [0002] Sulfated polysaccharides refer to polysaccharides in which the hydroxyl groups in the molecule are replaced by sulfate groups, also known as sulfated polysaccharides or polysaccharide sulfates. Natural sulfated polysaccharides are mainly derived from seaweed, heparin, plants and fungi, and have a wide range of biological activities. In addition to the most important anticoagulant effect, they also have blood lipid lowering, anti-aging, anti-tumor, immune regulation, hypoglycemic, Anti-radiation, anti-thrombosis and other biologically active functions. [0003] Ascophyllum nodosum belongs to Phaeophyta, and it is mainly born on the coast near the Arctic Ocean. Ascophyllum nodosum is a commonly used raw material in seaweed industry. It is used to extract ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08B37/00A61P1/00
CPCC08B37/006
Inventor 陈立根许伟邵荣颜秀花刘钱薇程德林
Owner 江门市方程健康生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com