Bacillus safensis and application in restoration of pollutants in open sea area

A technology of Bacillus suffolus and pollutants, applied in Bacillus suffolus and the application field of remediation of pollutants in open sea areas, can solve the problems of few demonstrations and projects, lack of microbial remediation methods and applications for sediment pollution in open sea areas, etc. , to achieve the effect of reducing the workload, ensuring the effect of delivery and repair, and increasing the probability of survival

Active Publication Date: 2017-05-31
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, microbial remediation of oil pollution in marine sediments is mostly at the level of theoretical research and small-scale laboratory simulation of marine environment experiments, and there are few demonstrations and projects of large-scale microbial remediation of oil pollution in offshore sediments.
On the one hand, due to the special marine environment, offshore construction has strict requirements on weather, sea conditions, construction vessels and offshore operating experience. Now most of the organic pollution in the ocean, especially oil pollution remediation, engineering demonstrations are mainly concentrated in tidal flat areas, lacking open sea areas Microbial remediation method and application of sediment pollution

Method used

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  • Bacillus safensis and application in restoration of pollutants in open sea area
  • Bacillus safensis and application in restoration of pollutants in open sea area
  • Bacillus safensis and application in restoration of pollutants in open sea area

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Screening and combination of high-efficiency petroleum-degrading bacteria

[0037] Using the microorganisms in the sediments of Yantai sea area as the source of high-efficiency oil-degrading bacteria, the indigenous strains with good degradation effect on oil pollution were screened, and the bacteria liquid was mixed according to the maximum degradation effect ratio, adsorbed on the surface of zeolite, and wrapped with a film agent. Subcontract delivery to complete the bioremediation of oil pollution in sediments in designated areas of the Bohai Sea.

[0038] (1) Bacterial screening: Weigh 2g of oil-contaminated seawater sediment samples and add them to the triangular flasks containing seawater culture medium and freshwater culture medium, respectively. 1 mL of culture medium was added to the screening medium, and 30 ℃, 180r / min constant temperature shaker was shaken for 5 days, then 1 mL of culture medium was pipetted and put into the new screening medium, an...

Embodiment 2

[0051] Example 2 Microbial Restoration of Oil Pollution in Sea Area Sediments

[0052] Remediation of oil pollution in sediments in the Bohai Sea.

[0053] (1) Restoration strains: Acinetobacter calcoaceticus, Pseudomonas putida and Bacillus saffron.

[0054] (2) Strain fermentation and mixing: The three strains were fermented on a large scale, respectively, and the concentration of the bacterial liquid after fermentation was 2-3OD. After fermentation, Acinetobacter calcoaceticus, Pseudomonas putida, and Bacillus saphobia were mixed according to the bacterial colony ratio of 3:2:4, and the total bacterial volume in the mixed bacterial solution was about 1×10 9 CFU / ml, mixed and stored in a refrigerator at 4 degrees Celsius.

[0055] (3) Adsorption and wrapping of bacterial liquid: the mixed bacterial liquid is mixed and adsorbed according to the mass ratio of bacterial liquid to zeolite 2:1, and the adsorption time is 2 hours. After the water content is 60%-70%, spray 7wt% ...

Embodiment 3

[0067] Embodiment 3 The method for the degradation of petroleum hydrocarbon by bacillus suffolk

[0068] (1) Microbial strains: Bacillus saphobia (Bb);

[0069] (2) Seed cultivation: select a ring of colonies from the plate and insert it into LB medium, the volume of liquid in the shaker is 30%, the cultivation temperature is 28-30°C, the cultivation speed is 180-200 rpm, and the cultivation time is 10-16h. Centrifuge at 13,000 rpm for 5 min to collect bacterial cells;

[0070] (3) Degradation of petroleum hydrocarbons by Bb: inoculate the Bb cells collected in step (2) with an initial concentration of 1 × 108 in an inorganic salt peptone liquid medium containing 2 g / L petroleum hydrocarbons for cultivation, and use uninoculated Bb The inorganic salt peptone liquid medium containing petroleum hydrocarbon was used as a control. After 7 days, the experimental group and the control group were taken, and the remaining petroleum hydrocarbons were extracted with petroleum ether, a...

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Abstract

The invention relates to the field of control of water environments by microorganisms, in particular to bacillus safensis and an application thereof. The bacillus safensis has been collected into Common Microorganism Center of China Committee for Culture Collection of Microorganisms on September 28th, 2016, with collection number of CGMCC No.13059. The method has the advantages that the indigenously-degradable strain is obtained through screening, is loaded, flocculated and encapsulated, and is applied to sea area to restore, so that the restoring strain can be furthest ensured to reach the specified submarine deposit during restoration, and the restoration effect of the strain is guaranteed; when the strain is fed, the complicated instrument equipment is not needed, and the feeding effect and restoration effect are guaranteed.

Description

technical field [0001] The invention relates to the field of water environment treatment by utilizing microorganisms, in particular to a bacillus saphobia and its application in repairing pollutants in open sea areas. Background technique [0002] Marine oil spills and oil pollution have always been important issues affecting the marine environment and marine ecology. At present, the emergency treatment of marine oil pollution, especially sudden oil spill accidents, is mainly aimed at the ocean surface seawater in the early stage of oil spills. Physical interception, recycling, chemical dispersants, etc. for emergency disposal. However, there is no good physical and chemical treatment methods for oil pollution on the seabed or the oil pollution that settles into seabed sediments with the movement of water bodies. The environmental self-purification of oil-degrading microorganisms in sediments and the artificial introduction of oil-degrading microorganisms are the current sol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07C12R1/01C12R1/40
CPCC02F3/34C02F2103/007C12N1/20C12N1/205C12R2001/07
Inventor 王巧宁陈令新付龙文孙西艳
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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