Light control technology for realizing cell serial adjustment by using dual-fiber optical tweezers

Abstract

The invention discloses an effective organization and adjustment scheme for realizing cell series based on dual-fiber optical tweezers. A photoetching substrate which is manufactured delicately and an operation depth limit are not required, so that potential sample pollution is avoided, and the operation and control range and the catching flexibility are enhanced effectively. Moreover, the position of the fiber optical tweezers is adjusted accurately, so that accurate operation and control of the cell series, including adjustment of the cell action distance, change of a cell connection sequence and the like can be realized.

Description

technical field [0001] The invention belongs to the field of optoelectronic technology, and in particular relates to an optical control technology for realizing cell serial adjustment by using double optical fiber optical tweezers. Background technique [0002] The interaction between adjacent cells is extremely important for the regulation of cell fate and function, including cell growth, tissue differentiation, intercellular signal transmission and mechanism force transmission, etc., which has attracted increasing attention of researchers. There are two main ways of intercellular interaction: exchange of soluble signaling molecules and direct intercellular contact. However, the above two modes of action are constantly changing with time and space. Specifically: different types of cells tend to have specific cell action modes, and the same type of cells has different modes of action at different distances. When two cells are in close contact, they tend to use the stress in...

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Problems solved by technology

[0004] Most of the currently reported cell serial adjustment schemes have certain limitations: in the process of cell serial organization based on microarrays and micromechanical systems, the cells are limited to the carefully crafted substrate surface, and the cells are in direct contact with the substrate surface Will cause potential cell contamination, which will affect the reuse of the system

For holographic optical tweezers, the design of holographic algorithm is more complicated, and the controllable adjustment of the specific arrangement of cells is more challenging: because it is necessary to manipulate a specific beam to move cells while keeping the positions of other beams unchanged, thus giving holographic Algorithm design adds challenges

In addition, holographic optical tweezers usually require complex optical focusing systems, including dichroic beam splitters, spatial beam modulators, and high numerical aperture focusing lenses, etc., which face challenges in the integrated application of micro-nano optoelectronic platforms; and the focusing lens is short The working distance further limits the depth of manipulation of cells in liquid environments

For plasmonic nano-optical tweezers, the specific cell assembly type still depends on the well-designed photolithographic nano-substrate. Therefore, once the cells are stably captured, the position is relatively fixed, and it is difficult to achieve effective dynamic adjustment.

Moreover, the cell capture range is limited to the substrate surface, which is not conducive to large-scale cell capture and manipulation

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