Light control technology for realizing cell serial adjustment by using dual-fiber optical tweezers

An optical fiber optical tweezers and dual-fiber technology, applied in the field of optical control technology, can solve the problems of affecting system reuse, complicated holographic algorithm design, fixed position, etc., and achieve the effect of avoiding holographic algorithm design, realizing reuse, and realizing dynamic adjustment.

Active Publication Date: 2017-05-31
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the currently reported cell serial adjustment schemes have certain limitations: in the process of cell serial organization based on microarrays and micromechanical systems, the cells are limited to the carefully crafted substrate surface, and the cells are in direct contact with the substrate surface Will cause potential cell contamination, which will affect the reuse of the system
For holographic optical tweezers, the design of holographic algorithm is more complicated, and the controllable adjustment of the specific arrangement of cells is more challenging: because it is necessary to manipulate a specific beam to move cells while keeping the positions of other beams unchanged, thus giving holographic Algorithm design adds challenges
In addition, holographic optical tweezers usually require complex optical focusing systems, including dichroic beam splitters, spatial beam modulators, and high numerical aperture focusing lenses, etc., which face challenges in the integrated application of micro-nano optoelectronic platforms; and the focusing lens is short The working distance further limits the depth of manipulation of cells in liquid environments
For plasmonic nano-optical tweezers, the specific cell assembly type still depends on the well-designed photolithographic nano-substrate. Therefore, once the cells are stably captured, the position is relatively fixed, and it is difficult to achieve effective dynamic adjustment.
Moreover, the cell capture range is limited to the substrate surface, which is not conducive to large-scale cell capture and manipulation

Method used

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  • Light control technology for realizing cell serial adjustment by using dual-fiber optical tweezers
  • Light control technology for realizing cell serial adjustment by using dual-fiber optical tweezers
  • Light control technology for realizing cell serial adjustment by using dual-fiber optical tweezers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1 The double-fiber optical tweezers used to adjust the serial order of cells and the manufacturing method of the experimental device thereof

[0053] The preparation of dual-fiber optical tweezers and its experimental equipment such as figure 2 Shown:

[0054] 1. Optical fiber optical tweezers FP 1 and 2 are prepared by the flame fusion tapered method:

[0055] In the first step, the single-mode fiber (connection type: FC / PC, core diameter: 9 μm, cladding diameter: 125 μm; Corning Inc.) is removed with fiber strippers to remove the buffer layer and polymer coating layer, and capillary Glass tube (inner diameter: ~0.9mm, tube wall thickness: ~0.1mm, length: ~120mm) to prevent fiber breakage and damage.

[0056] In the second step, heat the bare optical fiber above the flame of an alcohol lamp for about 1 minute, and stretch it along the optical axis after reaching the melting point of the optical fiber. Since fiber optic tweezers FP 1 and 2 have different ...

Embodiment 2

[0061] Example 2 The dual-fiber optical tweezers experimental device captures and migrates specific cells in the cell series

[0062] 1. The dual-fiber optical tweezers experimental device captures specific cells in the cell series

[0063] The specific experimental manipulation of cell series is as follows: image 3 Shown: when the 980nm laser is passed into the fiber optic tweezers FP 1 (P 1 =30mW), the Escherichia coli at the front end of FP 1 will be captured one by one under the action of light force, and a string of cells containing 6 Escherichia coli will be formed at the end of fiber optical tweezers FP 1 ( image 3 a). Next, a 980nm laser with a power of 50mW was passed into the fiber optic tweezers FP 2, and the fiber optic tweezers FP 2 was controlled to gradually approach the end of the cell series. At this time, E.coli 6( image 3 Middle black ring dotted line) rotates 50° counterclockwise and finally stabilizes its orientation along the axial direction of FP 2...

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Abstract

The invention discloses an effective organization and adjustment scheme for realizing cell series based on dual-fiber optical tweezers. A photoetching substrate which is manufactured delicately and an operation depth limit are not required, so that potential sample pollution is avoided, and the operation and control range and the catching flexibility are enhanced effectively. Moreover, the position of the fiber optical tweezers is adjusted accurately, so that accurate operation and control of the cell series, including adjustment of the cell action distance, change of a cell connection sequence and the like can be realized.

Description

technical field [0001] The invention belongs to the field of optoelectronic technology, and in particular relates to an optical control technology for realizing cell serial adjustment by using double optical fiber optical tweezers. Background technique [0002] The interaction between adjacent cells is extremely important for the regulation of cell fate and function, including cell growth, tissue differentiation, intercellular signal transmission and mechanism force transmission, etc., which has attracted increasing attention of researchers. There are two main ways of intercellular interaction: exchange of soluble signaling molecules and direct intercellular contact. However, the above two modes of action are constantly changing with time and space. Specifically: different types of cells tend to have specific cell action modes, and the same type of cells has different modes of action at different distances. When two cells are in close contact, they tend to use the stress in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/32
CPCG02B21/32
Inventor 李宝军刘晓帅张垚黄建滨
Owner JINAN UNIVERSITY
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