Method for purifying pharmaceutical proteins by aid of density difference processes
A protein and differential method technology, which is applied in the field of protein separation and purification, can solve the problems of long packing time for packed columns, expensive column chromatography materials, and limited quantity of pure products, so as to retain biological activity and solubility, save time and cost and The effect of low material cost and low cost
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Embodiment 1
[0047] A kind of method utilizing density difference method to purify bovine serum albumin of the present embodiment, comprises the following steps:
[0048] Step 1, take 10mL of bovine plasma containing 0.685% bovine serum albumin at normal temperature and pressure, add 50mL of 2MKH 2 PO 4The aqueous solution and 22mL of isobutanol were stirred in a blender at a speed of 500 rpm to 1000 rpm for 5 minutes, and mixed uniformly to obtain a mixed solution;
[0049] Step 2, using 0.1M KOH to adjust the pH value of the mixed solution obtained in Step 1 to 5.5±0.1;
[0050] Step 3: Pour the mixed solution after adjusting the pH value into the separator and let it stand for stratification for 5 minutes. It is divided into upper, middle and lower layers. The upper layer is an organic layer, the lower layer is an aqueous layer, and the middle layer is a bovine serum albumin layer. The middle layer got bovine serum albumin.
[0051] After determination, the purity of the bovine serum...
Embodiment 2
[0053] A kind of method utilizing density difference method to purify bovine serum albumin of the present embodiment, comprises the following steps:
[0054] Step 1, take 10mL of bovine plasma containing 0.685% bovine serum albumin at normal temperature and pressure, add 50mL of 2MKH 2 PO 4 Centrifuge the aqueous solution and 22mL isobutanol in a centrifuge at a speed of 500 rpm to 1000 rpm for 5 minutes, and mix evenly to obtain a mixed solution;
[0055] Step 2, using 0.1M KOH to adjust the pH value of the mixed solution obtained in Step 1 to 5.5±0.1;
[0056] Step 3: Pour the mixed solution after adjusting the pH value into the separator and let it stand for stratification for 5 minutes. It is divided into upper, middle and lower layers. The upper layer is an organic layer, the lower layer is an aqueous layer, and the middle layer is a bovine serum albumin layer. The middle layer got bovine serum albumin.
[0057] After determination, the purity of the bovine serum album...
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