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Method for preparing soapnut saponin by two-step method

A technology of sapindus sapindis and one-step method, applied in the field of two-step preparation of sapindus sapinis, can solve the problems of few practical application research, lack of detection methods for total sapindus saponins, etc., and achieve the effect of simple operation and rapid detection

Inactive Publication Date: 2017-06-13
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a two-step method for preparing Sapindus saponins in order to solve the problems that there are no sapindus sapindus quality control products sold on the market, the detection method of sapindus sapindus total saponins is missing, and there are few practical application studies

Method used

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  • Method for preparing soapnut saponin by two-step method
  • Method for preparing soapnut saponin by two-step method
  • Method for preparing soapnut saponin by two-step method

Examples

Experimental program
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Effect test

Embodiment 1

[0022] 1) Macroporous resin adsorption separation (primary macroporous resin separation)

[0023] Sapindus fruit peel is used as raw material, crushed, extracted with alcohol for 2 to 3 times, combined with sapinsia extract, and spray-dried to obtain sapinsia saponin coarse powder. Accurately weigh 0.5001g of sample powder, dilute to 100ml with deionized water, ultrasonically for 15min, put on D101 resin column, wash with 300ml of deionized water to remove sugar, wash with 100ml of 20% alcohol to remove other impurities, wash with 100ml of 70% alcohol to collect saponins, Rotary evaporation, after evaporation of solvent, received saponin coarse powder. The 70% alcohol wash can be used twice, and 0.5ml and 0.1ml of the two 70% alcohol washes are used to measure the absorbance values ​​of saponins and total sugars respectively with a UV spectrophotometer, and substitute them into the respective relational formulas:

[0024] The relational formula between absorbance value (A) an...

Embodiment 2

[0037] 1) Macroporous resin adsorption separation (twice macroporous resin separation)

[0038] Sapindus fruit peel is used as raw material, crushed, extracted with alcohol for 2 to 3 times, combined with sapinsia extract, and spray-dried to obtain sapinsia saponin coarse powder. Accurately weigh 1.0g of sample powder, dilute to 250ml with deionized water, ultrasonically for 15min, put on D101 resin column, wash with 300ml of deionized water to remove sugar, wash with 100ml of 20% alcohol to remove other impurities, wash with 100ml of 70% alcohol to collect saponins, Rotary evaporation, after evaporating the solvent, the acetone settled, centrifuged, evaporating the acetone, adding deionized water to dissolve, and extracting with saturated water n-butanol three times, taking the organic layer and evaporating the solvent, and preparing a 20 mg / mL aqueous solution of sapindus saponin, Apply D101 macroporous resin at a speed of 1mL / min, wash with 3BV of deionized water and elute ...

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Abstract

The invention provides a method for preparing soapnut saponin by a two-step method and relates to soapnut saponin. The method comprises the following steps: performing macroporous resin adsorption and separation; performing foam separation to obtain the soapnut saponin. The high-purity soapnut saponin is extracted and separated by the macroporous resin and foam separation two-step method, a method which is simple in operation and can quickly detect soapnut total saponin is designed, a basis is provided for quality control of the soapnut saponin, and research on extraction and separation industrialized application of the soapnut saponin is propelled. The soapnut saponin consists of an oleophilic aglycone part and hydrophilic glycosyl. The oleophilic aglycone part enables the soapnut saponin to be adsorbed by adsorbing resin to perform first-step separation. The soapnut saponin is a natural surfactant, has surface activity, is a necessary condition of foam separation, generates foam under the airflow bubbling action, and in the second step, the high-purity soapnut saponin product is refined through foam separation and separation of other impurities.

Description

technical field [0001] The invention relates to sapindus saponin, in particular to a two-step method for preparing sapindus saponin. Background technique [0002] Sapindus saponins mainly include triterpene saponins and some sesquiterpene glycosides with hedera saponin as the basic skeleton. They are a kind of natural non-ionic surfactants, which have antibacterial, bactericidal, anti-inflammatory, foaming and strong decontamination capabilities, and can be used as detergents. The separation and purification of Sapindus is the basis of its development and utilization. At present, the extraction and separation methods include macroporous resin separation method, extraction method and ultrafiltration method. Wei Fengyu et al. ([1] Wei Fengyu, Yu Jincheng, Xie Hui. Extraction and separation of natural Sapindus saponins[J]. Anhui Chemical Industry, 2007, 33(3): 15-17) adopted water extraction-macroporous resin adsorption separation method and Sapindus saponins are separated an...

Claims

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Application Information

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IPC IPC(8): C07J63/00G01N21/33G01N21/31G01N27/62
CPCC07J63/008G01N21/31G01N21/33G01N27/62
Inventor 黄加乐苏曦瑶王海涛李清彪孙道华
Owner XIAMEN UNIV
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