Arthrobacter globosa strain hch-1 that efficiently degrades 2-hydroxypyridine and its preparation method and application of bacterial agent
A technology of Arthrobacter globosa and hydroxypyridine, which is applied in the field of microbial degradation of pyridine compounds, can solve the problems of long growth delay, long treatment cycle, and low tolerance concentration, and achieve mild treatment conditions, extended adaptation time, and stable performance. Effect
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Embodiment 1
[0027] Example 1: Arthrobacter sphaericus Arthrobacter globiformils Isolation and purification of HCH-1
[0028] About 5 g of oil-contaminated soil samples from Liaohe Estuary were added to 50 mL of enrichment medium containing 1 mg / mL 2-hydroxypyridine, and after culturing for 5 days in a constant temperature shaking incubator at 30 °C and 170 rpm, 5 mL of enriched medium was taken. The culture solution was inoculated into a fresh enrichment medium containing 2-hydroxypyridine, and the process was repeated 4-5 times, and the final enriched culture solution was spread on the LB solid medium plate and placed in a 30°C constant temperature incubator Cultured for 48 hours, single colonies with different morphological characteristics were selected and inoculated in the screening medium containing 1 mg / mL 2-hydroxypyridine for verification, and the strains that grew better in the 2-hydroxypyridine screening medium were selected, among which One strain, named HCH-1, was preserved...
Embodiment 2
[0029] Example 2: Arthrobacter sphaericus Arthrobacter globiformils Identification of HCH-1
[0030] 1. Morphological, physiological and biochemical characteristics of the strain
[0031] The colony morphology of strain HCH-1 on LB solid plate is as follows figure 1 It is white, translucent, with wet surface and neat edges. When cultured in 2-hydroxypyridine-MSN medium, the color of the medium changes from colorless and transparent to blue-purple and then to brown-yellow. After identification of the strain, it was found that the medium was blue-purple when the strain was in the logarithmic phase, and the medium turned brownish yellow when it entered the stationary phase. The strains at different growth stages (logarithmic phase and stable phase) were all positive for Gram staining, and the morphology was spherical under the light microscope, and the scanning electron microscope showed that the strains were short rod-shaped, such as figure 2 As shown, the length is about...
Embodiment 3
[0036] Example 3: Arthrobacter sphaericus Arthrobacter globiformils Degradation of 2-Hydroxypyridine in Different Concentrations of Inorganic Salt Culture Medium by HCH-1
[0037] In the application of the strain HCH-1 in degrading 2-hydroxypyridine, the growth temperature is 18-42° C., the pH is 5-9, and the optimum growth temperature is 30° C. and the optimum pH is 7. Therefore, in this example, a temperature of 30° C. and a pH of 7 were selected for testing.
[0038] The Arthrobacter sphaericus strain HCH-1 was inoculated into LB liquid medium for activation for 48 h as seeds, and inoculated to 2-hydroxypyridine concentrations of 1 mg / mL, 2 mg / mL, and 4 mg according to the inoculum of 1%. / mL and 8 mg / mL inorganic salt medium (50 mL), placed in a constant temperature shaking shaker at 30 °C and 170 rpm for cultivation, sampling every 2 hours, and measuring the OD of bacterial growth. 600nm The absorption value and the concentration of 2-hydroxypyridine, and the degradat...
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