Preservation solution of time-resolved fluorescent microsphere labeled myoglobin antibody

Abstract

The invention discloses a preservation solution of a time-resolved fluorescent microsphere labeled myoglobin antibody. The preservation solution is prepared from the following components in percentage by mass: 0.1 percent to 1.5 percent of 4-(2-hydroxyethyl)piperazine-1-erhanesulfonic acid, 1.0 percent to 3.0 percent of sodium chloride, 0.3 percent to 0.8 percent of potassium chloride, 0.08 percent to 0.9 percent of disodium ethylene diamine tetraacetate, 0.01 percent to 0.06 percent of sodium dodecyl sulfonate, 0.1 percent to 3.0 percent of bovine serum albumin, 0.05 percent to 0.5 percent of glucan, 0.02 percent to 0.2 percent of triton, 0.01 percent to 0.05 percent of Tween-20, 0.02 percent to 0.1 percent of a preservative and the balance of water; the pH (Potential of Hydrogen) is adjusted to be 8.0 to 9.0. The preservation solution has the advantages that the raw materials are low in cost and easy to obtain, and a relatively stable microenvironment is provided for preservation of fluorescent microspheres.

Description

technical field [0001] The invention relates to the technical field of biochemical preparations, in particular to a time-resolved fluorescent microsphere-labeled myoglobin antibody preservation solution. Background technique [0002] In acute myocardial injury, myoglobin (MYO) is first released into the blood, and 2-3 hours after the onset of symptoms, the blood myoglobin (MYO) can exceed the normal upper limit, and reach the peak at 9-12 hours, 24 - Recovery after 36 hours. Although positive MYO cannot confirm the diagnosis of AMI, it can be used as an important indicator to exclude the diagnosis of AMI in the early stage. If MYO is negative, myocardial infarction can be basically ruled out. For the accuracy and stability of the detection, the diluent of the labeled antibody used in the final test is a key step. [0003] Time-resolved fluorescence biochemical analysis technology is established based on the special fluorescence properties of rare earth fluorescent complexe...

AI Technical Summary

Problems solved by technology

However, the stability of the fluorescent microspheres plays a key role in the subsequent detection steps. If the fluorescent microspheres are improperly stored, subsequent detection will not be possible, affecting the performance of the final detection reagent and bringing serious risks to reagent detection.

[0004] The time-resolved fluorescent microsphere-labeled myoglobin antibody (hereinafter referred to as labeled antibody) preservation solution is very important to maintain the stability of the labeled antibody in the liquid and prevent it from precipitation. There is no report on the labeled antibody preservation solution.

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