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Nano silk fibroin powder and preparation method thereof

A technology of nano silk and glue silk fibroin, applied in the field of silk products, can solve the problems of low whiteness, reduced whiteness, large particle size of silk fibroin powder, etc., and achieve the effect of obvious functional characteristics, excellent active function and large specific surface area.

Active Publication Date: 2017-06-23
NANTONG LOVER APPL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the existing method for preparing silk fibroin, an alkaline solution is used, which will destroy some amino acids in silk fibroin, especially serine and tyrosine; and in order to meet the whiteness requirements of cosmetic materials, during the preparation process, Bleach is used, and tests have shown that the whiteness of the product produced by this method is relatively low, and its whiteness will be significantly reduced after one year of storage
In particular, the existing method for preparing silk fibroin has a low yield, and the obtained silk fibroin powder has a large particle size

Method used

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  • Nano silk fibroin powder and preparation method thereof

Examples

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Effect test

Embodiment 1

[0032] Embodiment 1 A kind of preparation method of nano silk fibroin powder, comprises the following steps:

[0033] (1) Using mulberry silk as raw material, after removing impurities and rinsing, add pure water to soak for 30 minutes, the bath ratio is 10:1; then dehydrate to obtain cleaned silk; degumming the cleaned silk under high temperature and high pressure to obtain degummed silk cellulose fiber solution (5wt%); the pressure of the high-temperature degumming is 0.15MPa, the temperature is 110 ℃, and the degumming time is 10 minutes; Trichoderma viride is added to the degummed silk fiber solution, and the enzymolysis solution is obtained after enzymatic hydrolysis; The dosage of neutral protease is 1200 activity units / g silk fibroin powder, the enzymolysis temperature is 35°C, the enzymolysis time is 3.5 hours, and the pH value is controlled at 6.5;

[0034] (2) The enzymolysis solution is subjected to wall-breaking treatment; and then subjected to ultrasonic treatment...

Embodiment 2

[0037] Embodiment 2 A kind of preparation method of nano silk fibroin powder, comprises the following steps:

[0038](1) Using mulberry silk as raw material, after removing impurities and rinsing, add pure water to soak for 40 minutes, the bath ratio is 20:1; then dehydrate to obtain cleaned silk; degumming the cleaned silk under high temperature and pressure to obtain degummed silk cellulose fiber solution (20wt%); the pressure of the high-temperature degumming is 0.3MPa, the temperature is 135 ° C, and the degumming time is 20 minutes; Trichoderma viride is added to the degummed silk fiber solution, and the enzymolysis solution is obtained after enzymatic hydrolysis; The dosage of neutral protease is 1600 activity units / g silk fibroin powder, the enzymolysis temperature is 40°C, the enzymolysis time is 6 hours, and the pH value is controlled at 7.5;

[0039] (2) The enzymolysis solution is subjected to wall-breaking treatment; then through ultrasonic treatment, the treatment...

Embodiment 3

[0042] Embodiment three A kind of preparation method of nano silk fibroin powder, comprises the following steps:

[0043] (1) Using mulberry silk as raw material, after removing impurities and rinsing, add pure water to soak for 30 minutes, the bath ratio is 15:1; then dehydrate to obtain cleaned silk; degumming the cleaned silk under high temperature and pressure to obtain degummed silk cellulose fiber solution (45wt%); the pressure of the high-temperature degumming is 0.2MPa, the temperature is 125 ° C, and the degumming time is 15 minutes; Trichoderma viride is added to the degummed silk fiber solution, and the enzymolysis solution is obtained after enzymatic hydrolysis; The dosage of neutral protease is 1400 activity units / g silk fibroin powder, the enzymolysis temperature is 38°C, the enzymolysis time is 5 hours, and the pH value is controlled at 7;

[0044] (2) The enzymolysis solution is subjected to wall-breaking treatment; then after ultrasonic treatment, the treatmen...

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Abstract

The invention discloses nano silk fibroin powder and a preparation method thereof. The preparation method comprises the following steps: (1) adding neutral protease into degummed fibroin fiber solution, and carrying out enzymolysis, thus obtaining enzymatic hydrolysate, wherein a use quantity of the neutral protease is 1,200 to 1,600 activity units per gram of silk fibroin powder, an enzymolysis temperature is 35 to 40 DEG C, enzymolysis time is 3.5 to 6 hours, and a pH value is controlled into a range of 6.5 to 7.5; (2) carrying out wall breaking processing on the enzymatic hydrolysate, and then carrying out ultrasonic processing to obtain processed solution; (3) carrying out microwave heating on the processed solution, and then carrying out centrifugal washing to obtain washes; (4) concentrating the washes to prepare concentrate; (5) carrying out supergravity processing on the concentrate, then carrying out freeze drying, and carrying out irradiation sterilization to obtain the nano silk fibroin powder. Various indexes of a final product obtained by the preparation method disclosed by the invention, such as ash content, lead, mercury, arsenic, a total bacterial count and an escherichia coli content, are all greatly reduced; the finished product has high biological activity; an unexpected effect is obtained; the finished product is more suitable for a biological material.

Description

technical field [0001] The invention relates to a silk product, in particular to a nano silk fibroin powder and a preparation method thereof. Background technique [0002] The main body of silk fiber is silk fibroin, and silk fibroin powder is coated on the periphery of silk fibroin, and silk fibroin powder plays a protective and adhesive role on silk fibroin. The use of silk powder is mainly the trend of silk beauty that has become popular in the world in recent years. Although the concept of silk beauty is not very clear in China at present, but in foreign countries, especially in Japan, silk beauty has become a household name, and everyone knows that it is used in conjunction with pearl powder to form a series of pearl silk beauty powder. , has been widely acclaimed in the market. [0003] In the existing method for preparing silk fibroin, an alkaline solution is used, which will destroy some amino acids in silk fibroin, especially serine and tyrosine; and in order to m...

Claims

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Application Information

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IPC IPC(8): C12P21/06A61L15/32A61L15/64
CPCA61L15/32A61L15/64C12P21/06
Inventor 周小进胡小明张立新潘世俊石继均梅德祥盛家镛
Owner NANTONG LOVER APPL