Orientated surface enhanced Raman spectrum detection method based on high-activity site

A surface-enhanced Raman and spectral detection technology, which is applied in Raman scattering, measuring devices, and material analysis through optical means, can solve problems such as focus shaking, a sample that cannot be tested repeatedly, and unstable detection systems. , to achieve the effect of reducing requirements, easy focusing, and simple operation process

Active Publication Date: 2017-06-23
安徽中科赛飞尔科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above methods are usually tested in a liquid state, not only the focus is easy to shake due to the external environment, but also the detection system is unstable, and a sample cannot be tested repeatedly

Method used

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  • Orientated surface enhanced Raman spectrum detection method based on high-activity site
  • Orientated surface enhanced Raman spectrum detection method based on high-activity site
  • Orientated surface enhanced Raman spectrum detection method based on high-activity site

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A directional surface-enhanced Raman spectroscopy detection method based on highly active sites, comprising the following steps:

[0038] (1) Select a clean silicon wafer as a SERS detection substrate;

[0039] (2) After centrifugation, the silver nanosol with a particle size of 50 nm and SERS activity was synthesized, and concentrated at a ratio of 1:200;

[0040] (3) Dissolve the heroin to be tested into a solution;

[0041] (4) Add 2 μL of sodium chloride solution with a concentration of 0.25 mol / L, 2 μL of heroin solution to be tested, and 1 μL of concentrated silver nano-sol successively onto the substrate treated in step (1) and mix;

[0042] (5) Dry and form a film under the conditions of 24°C, RH= 50% constant temperature and humidity;

[0043] (6) SERS detection was performed on the film layer sample on the substrate by using a confocal microscopic Raman spectrometer; the excitation light wavelength of the Raman spectrometer was 633nm, and the spectrum was co...

Embodiment 2

[0055] A directional surface-enhanced Raman spectroscopy detection method based on highly active sites, comprising the following steps:

[0056] (1) Select a clean silicon wafer as the SERS detection substrate;

[0057] (2) The synthetic silver nanosol with a particle size of 40 nm and SERS activity was centrifuged and concentrated at a ratio of 1:100;

[0058] (3) Dissolving the amphetamine to be tested into a solution;

[0059] (4) Add 2 μL of 0.5 mol / L sodium chloride solution, 2 μL of amphetamine solution of the analyte, and 1 μL of concentrated silver nano-sol onto the substrate treated in step (1) successively and mix;

[0060] (5) Dry and form a film under the conditions of 24°C, RH= 50% constant temperature and humidity;

[0061] (6) SERS detection was performed on the film layer sample on the substrate by using a confocal microscopic Raman spectrometer; the excitation light wavelength of the Raman spectrometer was 531nm, and the spectrum was collected at a distance of...

Embodiment 3

[0065] A directional surface-enhanced Raman spectroscopy detection method based on highly active sites, comprising the following steps:

[0066] (1) Select a clean glass sheet as the SERS detection substrate for backup;

[0067] (2) The synthetic silver nanosol with a particle size of 50 nm and SERS activity was centrifuged and concentrated at a ratio of 1:150;

[0068] (3) Dissolving the test substance cocaine into a solution;

[0069] (4) Add 2 μL of sodium chloride solution with a concentration of 0.2 mol / L, 2 μL of cocaine solution to be tested, and 1 μL of concentrated silver nano-sol in sequence on the substrate treated in step (1) and mix;

[0070] (5) Dry and form a film under the conditions of 24°C, RH= 50% constant temperature and humidity;

[0071] (6) SERS detection was performed on the film layer sample on the substrate by using a confocal microscopic Raman spectrometer; the excitation light wavelength of the Raman spectrometer was 785 nm, and the spectrum was c...

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Abstract

The invention discloses an orientated surface-enhanced Raman spectrum detection method based on a high-activity site. The orientated surface enhanced Raman spectrum detection method comprises the following steps: selecting a clean silicon wafer or glass sheet as an SERS (surface-enhanced Raman scattering) detection chip for later use; synthesizing silver nano-sol with SERS activity and a grain diameter of 10nm to 60nm; after centrifuging, concentrating according to a ratio ranging from (1 to 100) to (1 to 400); dissolving an object to be detected into a solution; sequentially dropwise adding a sodium chloride solution, the solution of the object to be detected and the concentrated silver nano-sol into a substrate treated by the step (1) and mixing; storing a mixture under constant-temperature and constant-moisture conditions and drying to form a film; carrying out SERS detection on a film layer sample on the substrate by utilizing a microscopic confocal Raman spectrometer. The orientated surface enhanced Raman spectrum detection method is used for detecting under a dry state; Raman laser is more easily focused and is not easily interfered by air movement and the like; a sodium chloride crystal is visible under an optical microscope, so that a detection range can be effectively locked; a sample can be repeatedly detected for a plurality of times.

Description

technical field [0001] The invention relates to a surface-enhanced Raman spectroscopy (SERS) detection technology, in particular to a directional surface-enhanced Raman spectroscopy detection method based on highly active sites. Background technique [0002] Surface-Enhanced Raman Scattering (SERS) technology combines the inherent characteristics of Raman spectroscopy, that is, it can provide detailed structural information of molecules, and at the same time, due to the amplification of nanostructure optical signals, SERS can provide ultra-high detection sensitivity , even at the single-molecule level. In addition, SERS detection conditions are mild, the operation is simple, no sample pretreatment is required, and real-time in situ rapid detection can be realized. Therefore, SERS technology has developed into an analysis and detection method widely used in various fields. At present, the factors affecting the detection effect of this technology mainly include the followi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 杨良保于博荣毛妹孟娟陈诚曹晓敏唐祥虎
Owner 安徽中科赛飞尔科技有限公司
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