Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of oleanane type triterpene saponin compound and its preparation method and application

A technology of triterpene saponins and oleanane type, which is applied in the application field of pentacyclic triterpene compounds in the preparation of drugs for the treatment of inflammatory vascular diseases, and can solve the anti-inflammatory activity of oleanane type triterpene saponin compounds Unsatisfactory and other issues

Active Publication Date: 2018-11-16
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some scholars (Wu C, LiYL. Triterpenoid Saponins from the stem Barks of Schefflera heptaphylla. PlantaMed. 2013, 79: 1348-1355) found two oleanane-type triterpenoids Heptoleoside B from the bark of Schefflera heptaphylla. and C, in the LPS-induced RAW264.7 cell model, at a concentration of 40 μM, the inhibitory rates to NO were 22.5% and 45.2%, respectively, but the anti-inflammatory activity of the two oleanane-type triterpene saponins remained not ideal

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of oleanane type triterpene saponin compound and its preparation method and application
  • A kind of oleanane type triterpene saponin compound and its preparation method and application
  • A kind of oleanane type triterpene saponin compound and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0038] 1. Preparation of compounds

[0039] (1) Get the roots of Ilex pubescens, reflux extraction with 12, 10 and 8 times of 70% ethanol successively for 2 hours, combine the ethanol extracts, recycle the ethanol after filtration and concentrate under reduced pressure until there is no alcohol smell, to obtain the total extract 2.2 kg;

[0040] (2) Dissolve the total extract in water, pass through a D-101 macroporous resin column, and elute successively with 30% ethanol, 60% ethanol and 95% ethanol; the collection concentration is 60% % ethanol eluent was recovered under reduced pressure to obtain 566g thick extract, which was passed through a 200-300 mesh silica gel column, and carried out with chloroform-methanol as the solvent according to the gradient of 100:1-0:1 in chloroform:methanol Elution; collect the eluate with chloroform:methanol ratio of 9:1, concentrate and pass through Sephadex LH-20 column, use methanol as the eluent for elution, collect the eluate and conce...

example 2

[0049] Example 2: (injection)

[0050] Take 1000 mg of the compound obtained by the method described in Example 1 above, add 1000 ml of water for injection, adjust the pH value to 7-7.5 with sodium carbonate, stir to dissolve, sterilize and filter, potting, and circulate steam at 100 ° C for 15 minutes Sterilize and make each 2mg / 2ml injection for injection.

example 3

[0051] Example 3: (capsules)

[0052] Take the compound 5000mg obtained by the method described in the above-mentioned Example 1 and fully mix with auxiliary materials such as 4000mg microcrystalline cellulose, 500mg sodium carboxymethyl starch, 400mg sodium lauryl sulfate, and carry out dry granulation by roller compaction, and then mix with An appropriate amount of magnesium stearate is mixed, filled into 3# hollow capsules, and made into capsules with a specification of 100 mg / grain for oral use.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
control rateaaaaaaaaaa
control rateaaaaaaaaaa
Login to View More

Abstract

The invention relates to oleanane triterpene saponin compounds of which the molecular structure is disclosed as Formula (I). The preparation method comprises the following steps: carrying out ethanol reflux extraction on hairy holly root, and carrying out column chromatography separation purification. The compounds have anti-inflammatory activity, and can be used for preparing drugs for preventing and treating inflammatory vascular diseases.

Description

technical field [0001] The invention relates to a steroid compound, in particular to a pentacyclic triterpenoid compound and the application of the compound in the preparation of medicines for treating inflammatory vascular diseases. Background technique [0002] Ilex pubescens is a plant of the genus Ilex pubescens Hook.et Arn. in the family Aquifoliaceae. It is also known as Wuwei Ding, June Frost, Thin-leaf Holly, Topiary, Water Gunpowder, and Firewood, etc. It is mainly produced in Guangdong and Jiangxi , Fujian, Guangxi and other places are commonly used traditional Chinese medicines in southern my country. Its medicinal part is the root, which has the effects of promoting blood circulation and dredging collaterals, reducing swelling and pain, clearing heat and detoxifying, and is widely used clinically to treat coronary heart disease, angina pectoris, thromboangiitis obliterans, etc. [0003] Some literatures have reported the anti-inflammatory effect of Ilex pubescens...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00A61K31/704A61P29/00A61P9/00
CPCC07J63/008
Inventor 刘中秋吴鹏周华高慧朱丽君卢琳琳王莹戚笑笑王立萍
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE