Kit and application for detecting urinary pathogenic bacteria

A urine disease and reagent kit technology, applied in the field of medical testing, can solve problems such as high cost, high technical requirements, and low throughput detection, and achieve the effects of simple operation, high detection sensitivity, and shortened detection time

Active Publication Date: 2021-08-06
HANGZHOU DIAN BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its cost is relatively high, technical requirements are high, and the degree of throughput detection is low, so it is generally difficult for primary hospitals to accept it.

Method used

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  • Kit and application for detecting urinary pathogenic bacteria
  • Kit and application for detecting urinary pathogenic bacteria
  • Kit and application for detecting urinary pathogenic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: the standard curve of pathogenic bacteria of urine sample.

[0043] Test strains: as follows (Table 1), purchased from Guangdong Microbial Culture Collection Center.

[0044] Table 1 Standard strains

[0045]

[0046] Urine source: The midsection urine of several healthy volunteers was collected and mixed for use.

[0047]Urine pathogenic bacteria ATP detection kit includes urine pretreatment reagents, pathogenic bacteria ATP lysate, neutralizing solution, and ATP detection reagents. The urine pretreatment reagent is composed of somatic cell digestion solution and ATPase, the composition of somatic cell digestion solution is 0.3-0.7% Triton and 0.03-0.07% TCA, the ATPase is 95-105U / mL APYrase; the ATP lysate of pathogenic bacteria is 2-5% TCA; the neutralizing solution is composed of 100-123.4mmol / LTris-base, 2-2.34mmol / L magnesium acetate, and the pH is adjusted to 8.2; the ATP detection reagent is composed of luciferase, luciferin and buffer, The c...

Embodiment 2

[0058] Example 2: Determination of the critical value for the detection of pathogenic bacteria ATP in urine samples.

[0059] Urine specimen collection: 224 cases of urine specimens were collected from the Department of Microbiology of our hospital.

[0060] Urine pathogenic bacteria ATP detection kit includes urine pretreatment reagents, pathogenic bacteria ATP lysate, neutralizing solution, and ATP detection reagents. The urine pretreatment reagent is composed of somatic cell digestion solution and ATPase, the composition of somatic cell digestion solution is 0.5% Triton by volume fraction and 0.05% TCA by mass fraction, and APYrase with 100U / mL ATPase; The mass fraction is 4% TCA; the neutralizing solution is composed of 123.4mmol / L Tris-base, 2.34mmol / L magnesium acetate, and the pH is adjusted to 8.2; the ATP detection reagent is composed of luciferase, luciferin and buffer solution, of which The concentration of luciferase was 16 μg / mL, the concentration of luciferin wa...

Embodiment 3

[0071] Example 3: Detection of ATP of pathogenic bacteria in clinical urine samples.

[0072] Urine specimen collection: 1055 urine specimens were collected from three tertiary hospitals.

[0073] Urine pathogenic bacteria ATP detection kit includes urine pretreatment reagents, pathogenic bacteria ATP lysate, neutralizing solution, and ATP detection reagents. The urine pretreatment reagent is composed of somatic cell digestion solution and ATPase, the composition of somatic cell digestion solution is 0.5% Triton by volume fraction and 0.05% TCA by mass fraction, and APYrase with 100U / mL ATPase; 4% TCA; the neutralizing solution is composed of 123.4mmol / L Tris-base, 2.34mmol / L magnesium acetate, and the pH is adjusted to 8.2; the ATP detection reagent is composed of luciferase, luciferin and buffer solution, of which luciferase The concentration was 16 μg / mL, the concentration of fluorescein was 20 μg / mL, the buffer was composed of 20 mmol / L Tris-base and 2 mmol / L magnesium ac...

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Abstract

The invention discloses a kit for detecting urinary pathogenic bacteria, including urine pretreatment reagents, pathogenic bacteria ATP lysate, neutralization solution, and ATP detection reagents. It is fast, accurate and sensitive, and provides a reliable method for clinical diagnosis of urinary tract infection, which can reduce clinical ineffective medication and improve the accuracy of medication.

Description

technical field [0001] The invention belongs to the technical field of medical testing, and in particular relates to a kit for detecting urinary pathogenic bacteria and its application. Background technique [0002] According to the statistics of the World Organization, infectious diseases are still the highest incidence and the main cause of human death. In the past 30 years, due to the irrational clinical application of a large number of broad-spectrum antibiotics, the drug resistance of clinical disease-related microorganisms (including bacteria, viruses, and certain parasites) has continued to increase, resulting in the imbalance of normal flora and the emergence of a large number of drug-resistant strains. For clinical microbial infections, especially the more common pathogenic bacterial infections, quickly and accurately judge whether there is pathogenic bacterial infection, on the one hand, it is conducive to further clinical detection and treatment in a timely manner...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/66C12Q1/04
CPCC12Q1/04C12Q1/66C12Q2304/60
Inventor 虞闰六任绪义施宏杨狄
Owner HANGZHOU DIAN BIOTECH CO LTD
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