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Nano composite of polynucleotide and gold nano rod, and preparation method and applications thereof

A polynucleotide and nanocomposite technology, which can be applied to preparations for in vivo tests, medical preparations containing active ingredients, and drug combinations, etc., can solve the problems that the efficiency needs to be further optimized and improved.

Inactive Publication Date: 2017-07-04
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of lutamine metal-organic complexes in the field of siRNA delivery needs to be further studied, especially the efficiency of gene release behavior needs to be further optimized and improved

Method used

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  • Nano composite of polynucleotide and gold nano rod, and preparation method and applications thereof
  • Nano composite of polynucleotide and gold nano rod, and preparation method and applications thereof
  • Nano composite of polynucleotide and gold nano rod, and preparation method and applications thereof

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Experimental program
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Effect test

preparation example

[0029] 1) Synthesis of lipoic acid modified polynucleotide carrier compound

[0030]

[0031] At room temperature, a catalytic amount of concentrated sulfuric acid (1.0 mL) was added dropwise to a solution of compound 1 (18.2 g, 0.1 mol) in ethanol (100 mL), and the reaction was stirred under reflux overnight. Cooled to room temperature, evaporated to dryness under reduced pressure to obtain 21.2 g of compound 2, yield: 89%.

[0032]

[0033] Under nitrogen protection, under an ice-salt bath, a solution of compound 2 (21.2 g, 0.089 mol) in anhydrous tetrahydrofuran (80 mL) was slowly added dropwise to LiAlH 4 (10.1g, 0.267mol) in anhydrous tetrahydrofuran (50mL) suspension, the temperature was maintained at around 0°C. After the dropwise addition, the reaction was maintained at 0° C. and stirred for 30 minutes, then returned to room temperature, and stirred and reacted for 5 hours. Cool in an ice-salt bath, slowly add ice water (100mL) dropwise, keep the temperature be...

Embodiment example

[0052] 1. If figure 2 As shown, the uniform surface function modified GNR is obtained in the present invention. It can be seen by dynamic light scattering that the average diameter of GNR increases slightly from 48.6±6.8 to 51.13±5.2nm after complexation with dimethylpyridinium zinc, and increases to 84.1±8.6nm after combining with siRNA. And, with step-by-step modification, the surface potential decreased from 66.2±3.4 (GNR) to 24.5±5.2mV (ZD-GNR), and further to 2.3±1.0mV (siRNA / ZD-GNR). The agarose gel retardation test of siRNA / ZD-GNR was carried out by using firefly luciferase gene siRNA (siLuc) as a template siRNA ( figure 2 ). When the molar ratio of siRNA / ZD-GNR reached 100, ZD-GNR was completely combined with siRNA to form a nanocomplex. In addition, for the siLuc / ZD-GNR complex, the complexation between siRNA and Zn / DPA was destroyed after adding phosphate. Without zinc ions, lutamine-GNRs cannot complex with siRNA at any concentration. The results showed that ...

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Abstract

The invention discloses a nano composite of polynucleotide and gold nano rod, and a preparation method and applications thereof. The surface of a nano gold rod is modified by a polynucleotide carrier compound, which is modified by lipoic acid having high affinity with phosphoric acid molecules. The polynucleotide carrier compound and the gold nano rod modified by zinc ion metal organic compounds can be used as an effective carrier for gene therapy. Due to the strong coordinated complexing effect between the surface modified gold nano rod and treatment genes, the nano composite has a high stability, and in in-vitro and in-vivo experiments, the treatment genes can be efficiently delivered to the targeted tumor cells. Gene silencing and gold nano rod photo-thermal activity are used to achieve gene / photo-thermal synergic therapy on cancer.

Description

technical field [0001] The invention relates to a surface-modified gold nanorod as a gene carrier and applied to efficient gene-photothermal synergistic therapy for cancer, belonging to the fields of biomedical materials and nanomedicine. Background technique [0002] RNA interference (RNAi) is the use of specific gene sequences to cause gene silencing after transcription, and has great potential in cancer therapy. However, although small interfering RNA (siRNA) technology has emerged as an effective therapeutic option, the delivery of siRNA is limited due to the large anionic charge and molecular weight of the siRNA phosphate backbone, leading to poor pharmacokinetics after systemic administration. Kinetics and lower rate of cellular internalization. Therefore, siRNA preparations for tumor therapy have not been successfully used clinically so far. Although many siRNA delivery vehicles based on cationic polymers, anionic lipids or amino acids can effectively carry anionic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K41/00A61K49/22A61K31/7088A61P35/00
CPCA61K31/7088A61K41/0052A61K49/221A61K2300/00
Inventor 刘刚陈小元楚成超
Owner XIAMEN UNIV