Two black mustard repeated DNA sequences and application thereof

A technology of DNA molecules and sequences, which is applied to two kinds of black mustard genome DNA sequences and its application fields, can solve the problems of cumbersome methods, poor repeatability, and the identification is greatly affected by subjective factors, and achieve the effect of easy identification
CN106916895AActive Publication Date: 2017-07-04BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Publication Date
2017-07-04

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Abstract

The invention discloses two black mustard genome DNA sequences and application thereof, and provides a DNA molecule set used for identifying a B genome of brassica plants. The DNA molecule set is composed of a single stranded DNA molecule as shown in sequence 1 in a sequence table and a single stranded DNA molecule as shown in sequence 2 in the sequence table. Experiments prove that by obtaining BNSAT28 and BNSAT68, chromosomes of brassica plants can be identified more easily and more accurately, and the problem that no ideal probe is available for identification of chromosomes of brassica plants is solved.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to two genomic DNA sequences of black mustard and applications thereof. Background technique

[0002] Cruciferous Brassica vegetables include the A genome Brassica rapa (AA, n=10) represented by Chinese cabbage, the B genome of black mustard, Brassica nigra (BB, n=8), and the C genome Brassica oleracea represented by cabbage (CC, n=9), and three heterotetraploid compound species formed by their natural doubling: Brassica napus B.napus (AACC, n=19), Brassica napus B.juncea (AABB, n=18 ), and B. carinata (BBCC, n=17), formed the famous U-triangle. Contains the world's most important edible, oily and seasoning vegetables, and is also a model material for studying genetic diversity within the genus and the evolution of species and polyploidy.

[0003] Black mustard is a wild species of Brassica, which retains many excellent traits after long-term ecological adaptation, such as resistance ...

Claims

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