Method for enhancing luminescent properties of silver nanoclusters protected by double stranded DNA based on aggregation induced luminescence enhancement mechanism

A technology of aggregation-induced luminescence and silver nanoclusters, applied in the field of nanomaterials, can solve the problems of limited application and low fluorescence quantum yield

Inactive Publication Date: 2017-07-11
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, compared with quantum dots, its fluorescence quantu

Method used

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  • Method for enhancing luminescent properties of silver nanoclusters protected by double stranded DNA based on aggregation induced luminescence enhancement mechanism
  • Method for enhancing luminescent properties of silver nanoclusters protected by double stranded DNA based on aggregation induced luminescence enhancement mechanism
  • Method for enhancing luminescent properties of silver nanoclusters protected by double stranded DNA based on aggregation induced luminescence enhancement mechanism

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Experimental program
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Embodiment 1

[0024] Prepare double-stranded DNA-protected silver nanoclusters according to the literature method (Analyst, 2017, 142, 800-807): take the template strand DNA sequence 5′-TTTAAATAATATCCCTTAATCCCC-3′ (TTTAAATAATAT is the complementary chain, and CCCTTAATCCCC is the protection silver nano The template segment of the cluster) and the complementary DNA sequence 5′-GGGGTGGGGTGGGGTGGGATATTATTTAAA-3′ (ATATTATTTAAA is the complementary chain segment, GGGGTGGGGTGGGGTGGG is the G-base-rich segment) containing the complementary chain DNA sequence rich in G bases) to prepare DNA-protected silver nanoclusters Specific steps are as follows:

[0025] Method: Weigh silver nitrate (AgNO 3 ) 17.0mg, add 20mL distilled water to prepare 5mmol / L silver nitrate mother liquor for later use (keep away from light); weigh 358mg disodium hydrogen phosphate (Na 2 HPO 4 ·12H 2 O) Add 50mL water to prepare 20mmol / L disodium hydrogen phosphate solution; then weigh 156mg sodium dihydrogen phosphate (Na 2 HPO 4 ...

Embodiment 2

[0027] Establish the quantitative relationship between double-stranded DNA-protected silver nanoclusters and BSA: The double-stranded DNA-protected silver nanoclusters solution prepared in Example 1 was diluted to 10 times the volume with phosphate buffer solution, and the concentration was 1.0×10 -6 mol / L solution; respectively take 1mL of this solution and add BSA mother liquor to it to make the final concentration of BSA be 0~140×10 -6 mol / L(0,10,20,30,40,50,60,70,80,90,100,120,140×10 -6 mol / L), and 4 minutes later, the fluorescence emission spectra (excitation wavelength 470nm) of the silver nanocluster solution in response to different concentrations of BSA were recorded using a fluorescence spectrometer (Shimadzu, Japan, RF-5301PC). Such as figure 1 As shown, as the concentration of BSA increases, its fluorescence emission peak at 555nm is gradually enhanced and blue shifted to 522nm, and the intensity increases from 12 at 555nm to 70 at 522nm, an increase of about 6 times. ...

Embodiment 3

[0029] The double-stranded DNA-protected silver nanocluster solution prepared in Example 1 was diluted with phosphate buffer solution to 10 times the volume to make a concentration of 1.0×10 -6 mol / L solution, respectively take 11 parts of 1mL of this solution, and add BSA mother liquor to the final concentration of 10×10 -6 mol / L; 4min later, add trypsin mother solution to each aliquot to make the final concentration of 0~80ng / μL (0, 5, 10, 15, 20, 25, 30, 40, 50, 60, 70, 80ng / μL), 5min later, use a fluorescence spectrometer to record the fluorescence emission spectra (excitation wavelength of 470nm) of the mixed solution of silver nanoclusters protected by double-stranded DNA and BSA in response to different concentrations of trypsin. Such as image 3 As shown, as the concentration of trypsin increases, the intensity of the fluorescence emission peak at 522 nm gradually increases, from 35 at 522 nm to 120 at 545 nm, which is approximately 3.5 times stronger, and red shifts to 5...

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Abstract

The invention relates to a method for enhancing luminescent properties of silver nanoclusters protected by double stranded DNA based on an aggregation induced luminescence enhancement mechanism, and belongs to the technical field of nano materials. Metal nanoclusters used in the method are the silver nanoclusters protected by the double stranded DNA, the double stranded DNA comprises G-C segments, by use of the mechanism of electrostatic interaction of phosphoric acid groups in the G-C segments and serum protein, BSA is used for ligand DNA aggregation to limit vibration rotation of the ligand double stranded DNA of the silver nanoclusters to reduce internal consumption so as to induce fluorescence enhancement: further Trypsin is added for hydrolysis of the BSA and change of charge distribution on the surface of the BSA to induce further ligand DNA aggregation so as to further enhance fluorescence of the silver nanoclusters, and the luminescent properties of the silver nanoclusters can be enhanced by the aggregation induced luminescence enhancement way. Further a complex formed by combination of the silver nanoclusters protected by the double stranded DNA and the BSA can be used for quantitative detection of the trypsin, and through calculation, the detection sensitivity can reach 2.8ng / mu L.

Description

Technical field [0001] The invention belongs to the technical field of nanomaterials, and specifically relates to a method for improving the luminescence performance of silver nanoclusters protected by double-stranded DNA based on an aggregation-induced luminescence enhancement mechanism. Background technique [0002] Metal nanoclusters have been widely used in bioimaging and the detection of various in vitro and in vivo biomolecules due to their small size, controllable emission wavelength, good stability and good biocompatibility. But compared with quantum dots, its fluorescence quantum yield is still low, which severely limits its application. In recent years, it has been discovered that metal nanoclusters also have aggregation-induced fluorescence enhancement. This is mainly due to the fact that aggregation restricts the vibration and rotation of the ligand and reduces the internal friction, thereby increasing the fluorescence and accompanied by the shift of the peak position...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 李洪伟王威贤吴玉清
Owner JILIN UNIV
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