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A method to enhance the luminescent properties of double-stranded DNA-protected silver nanoclusters

A technology of silver nanoclusters and luminescence properties, applied in the field of nanomaterials, can solve the problems of low fluorescence quantum yield and limited application, etc.

Inactive Publication Date: 2019-03-26
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, compared with quantum dots, its fluorescence quantum yield is still low, which seriously limits its application.

Method used

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  • A method to enhance the luminescent properties of double-stranded DNA-protected silver nanoclusters
  • A method to enhance the luminescent properties of double-stranded DNA-protected silver nanoclusters
  • A method to enhance the luminescent properties of double-stranded DNA-protected silver nanoclusters

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Silver nanoclusters protected by double-stranded DNA were prepared according to the literature method (Analyst, 2017, 142, 800-807): the DNA sequence 5′-TTTAAATAATATCCCCTTAATCCCC-3′ of the template strand containing the complementary strand (TTTAAATAATAT is the complementary segment, CCCTTAATCCCC is the protected silver nanocluster The template segment of the cluster) and the complementary strand DNA sequence 5'-GGGGTGGGGTGGGGTGGGATATTTATTTAAA-3' containing G bases (ATATTATTTAAA is a complementary segment, GGGGTGGGGTGGGGTGGG is a G base-rich segment) to prepare DNA-protected silver nanoclusters, Specific steps are as follows:

[0025] Method: Weigh silver nitrate (AgNO 3 ) 17.0mg, add 20mL distilled water to configure 5mmol / L silver nitrate mother liquor for later use (keep away from light); weigh 358mg disodium hydrogen phosphate (Na 2 HPO 4 12H 2 O) add 50mL water configuration 20mmol / L disodium hydrogen phosphate solution; Then weigh 156mg sodium dihydrogen phosph...

Embodiment 2

[0027] Establish the quantitative relationship between the silver nanoclusters protected by double-stranded DNA and BSA: the silver nanocluster solution protected by double-stranded DNA prepared in Example 1 was diluted to 10 times the volume with phosphate buffer solution, and the concentration was 1.0×10 -6 mol / L solution; take 1mL of this solution, add BSA mother liquor to it respectively, so that the final concentration of BSA is 0~140×10 -6 mol / L (0, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 120, 140×10 -6 mol / L), and after 4min, use a fluorescence spectrometer (Shimadzu, Japan, RF-5301PC) to record the fluorescence emission spectra (excitation wavelength 470nm) of the silver nanocluster solution in response to different concentrations of BSA. Such as figure 1 As shown, with the increase of BSA concentration, its fluorescence emission peak at 555nm is gradually enhanced and blue-shifted to 522nm, and the intensity is enhanced from 12 at 555nm to 70 at 522nm, which is abou...

Embodiment 3

[0029] The silver nanocluster solution protected by double-stranded DNA prepared in Example 1 was diluted to 10 times volume with phosphate buffer solution to form a concentration of 1.0×10 -6 mol / L solution, take 12 parts of 1mL of the solution, add BSA mother liquor to it, so that the final concentration is 10×10 -6 mol / L; 4min later, add trypsin stock solution to each portion to make the final concentration of 0-80ng / μL (0, 5, 10, 15, 20, 25, 30, 40, 50, 60, 70, 80ng / μL), after 5min, use a fluorescence spectrometer to record the fluorescence emission spectra of the mixed solution of double-stranded DNA-protected silver nanoclusters and BSA in response to different concentrations of trypsin (excitation wavelength is 470nm). Such as image 3 As shown, with the increase of trypsin concentration, the fluorescence emission peak intensity at 522nm gradually increased, the intensity increased from 35 at 522nm to 120 at 545nm, about 3.5 times stronger, and red shifted to 545nm. S...

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Abstract

The invention relates to a method for enhancing luminescent properties of silver nanoclusters protected by double stranded DNA based on an aggregation induced luminescence enhancement mechanism, and belongs to the technical field of nano materials. Metal nanoclusters used in the method are the silver nanoclusters protected by the double stranded DNA, the double stranded DNA comprises G-C segments, by use of the mechanism of electrostatic interaction of phosphoric acid groups in the G-C segments and serum protein, BSA is used for ligand DNA aggregation to limit vibration rotation of the ligand double stranded DNA of the silver nanoclusters to reduce internal consumption so as to induce fluorescence enhancement: further Trypsin is added for hydrolysis of the BSA and change of charge distribution on the surface of the BSA to induce further ligand DNA aggregation so as to further enhance fluorescence of the silver nanoclusters, and the luminescent properties of the silver nanoclusters can be enhanced by the aggregation induced luminescence enhancement way. Further a complex formed by combination of the silver nanoclusters protected by the double stranded DNA and the BSA can be used for quantitative detection of the trypsin, and through calculation, the detection sensitivity can reach 2.8ng / mu L.

Description

technical field [0001] The invention belongs to the technical field of nanometer materials, and in particular relates to a method for improving the luminescence performance of double-stranded DNA-protected silver nanoclusters based on an aggregation-induced luminescence enhancement mechanism. Background technique [0002] Metal nanoclusters have been widely used in bioimaging and detection of various in vitro and in vivo biomolecules due to their small size, controllable emission wavelength, good stability and good biocompatibility. However, compared with quantum dots, its fluorescence quantum yield is still low, which seriously limits its application. In recent years, it has been found that metal nanoclusters also have aggregation-induced fluorescence enhancement, which is mainly due to the fact that aggregation limits the vibration and rotation of ligands, which reduces internal energy consumption, thereby enhancing fluorescence and accompanied by peak shifts. Therefore, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 李洪伟王威贤吴玉清
Owner JILIN UNIV
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