Phomopsis fungus strain E41 and applications thereof

A technology of phosphimase and fungus, which is applied to the fungal strain E41 of the genus Phomopsis and its application field in degrading the herbicide dimethyltetrachloride, and can solve the problem that pregnant women are prone to miscarriage, the content exceeds a safe value, and the natural environment is damaged. and other issues, to achieve the effect of important industrial application value, good market prospects and easy industrialization

Active Publication Date: 2017-07-14
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that excessive herbicide residues in plants can cause various diseases such as cancer, leukemia, kidney necrosis and abortion in pregnant women through eating, and can also cause abortion or even death in animals; the abundance of herbicides in the environment A large amount of soil and water are polluted and destroyed by herbicides, and their content exceeds the safe value; the herbicides left in the soil cause phytotoxicity of subsequent crops, which in turn leads to reduced production, and severe areas even cause production failure. In some areas, soil compaction due to the use of herbicides is no longer suitable for farming

Method used

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  • Phomopsis fungus strain E41 and applications thereof
  • Phomopsis fungus strain E41 and applications thereof
  • Phomopsis fungus strain E41 and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 : Isolation, screening and identification of Phomopsis sp. E41

[0021] 1.1 Experimental materials

[0022] Inorganic salt medium: 1 g of ammonium nitrate, 1.5 g of dipotassium hydrogen phosphate, 0.5 g of potassium dihydrogen phosphate, 0.1 g of crystalline magnesium sulfate, 45.72 mg of ferrous sulfate (7 water), 1 L of water, 40 g of agar.

[0023] LB medium: 10g tryptone, 5g yeast powder, 10g sodium chloride, 1L water, 40g agar.

[0024] PDA medium: 200g potatoes, 20g glucose, 500-600mL water, boiled for about half an hour, passed through gauze, dilute to 1 liter, add 40g agar.

[0025] The degradation solution is a mixture of inorganic salt medium and dimethyltetrachloride.

[0026] 1.2 Separation and identification methods

[0027] Phomopsis sp. E41 of the present invention is obtained from sampling, isolation and screening in Mingyang, Guangxi, and is preserved by the Guangdong Microbial Culture Collection Center with a preservation number of GDMCC...

Embodiment 2

[0041] Example 2 : Study on the effect of Phomopsis sp. E41 on the degradation of dimethyltetrachloride

[0042] 2.1 Cultivation of degrading bacteria

[0043] Prepare PDB medium, sterilize it, and put it in sterilized 30mL vials with caps, 10mL per bottle, scrape the purified fungal hyphae in PDA medium with an inoculation loop, and insert them into PDA medium vials respectively, To obtain expanded cultured cells, the culture condition is to cultivate at 28°C for 3-4 days and wait for use.

[0044] 2.2 Screening of degrading bacteria

[0045] Prepare the degradation test medium of degrading bacteria, and distribute it in sterile 30mL vials with caps, 10mL per bottle. Add 0.55g / L (by dry weight) to expand the cultured cells and mix with the subpackaged degrading bacteria degradation test medium. Before mixing, wash with the degradation bacteria degradation test medium for 3 times and then mix with the degradation test medium , three parallel treatments and a blank control...

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Abstract

The invention belongs to the technical field of pesticide degradation by endophytic bacteria, and specifically relates to a phomopsis fungus strain E41 and an application thereof in herbicide chipton degradation. The taxonomic name of the phomopsis fungus strain E41 is Phomopsis sp. E41. The phomopsis fungus strain E41 is preserved in Guangdong Culture Collection Center (GDMCC) in January 16th, 2017. The preservation number is GDMCC No.60143. The provided phomopsis sp. E41 can degrade herbicide chipton in water or soil, the cost is low, the industrialization is easy, the market prospect is good, and at the same time, the industrial application value is great.

Description

technical field [0001] The invention belongs to the technical field of plant endophyte degrading pesticides, and in particular relates to a Phomopsis fungus strain E41 and its application in degrading the herbicide dimethyltetrachloride. Background technique [0002] With the development of China's agricultural modernization, changes in the structure of agricultural planting, the development of agricultural management models in the direction of scale, industrialization, and intensification, and the reduction of rural labor force, the demand for herbicides in agriculture will continue to increase. At the same time, changes in agricultural cultivation methods have also contributed to a surge in the demand for herbicides. [0003] The use of herbicides has greatly increased crop yields, reduced the burden on farmers, and provided a guarantee for agricultural labor-saving and mechanized production. At the same time, the use of herbicides has also had a serious impact on people a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A62D3/02C02F3/34B09C1/10C12R1/645A62D101/04A62D101/22A62D101/28
CPCA62D3/02A62D2101/04A62D2101/22A62D2101/28B09C1/10C02F3/347C12N1/145C12R2001/645
Inventor 王彦辉曾东强封国君唐文伟李红红
Owner GUANGXI UNIV
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