Liquid chromatogram-inductively coupled plasma mass spectrometry detection method of vitamin B12

A liquid chromatography and inductive coupling technology, applied in the field of component detection, can solve the problems of low detection limit, matrix interference, and high detection limit, and achieve the effects of low detection limit, strong resistance to matrix interference, and easy operation.

Inactive Publication Date: 2017-07-14
HUBEI PROVINCIAL INST FOR FOOD SUPERVISION & TEST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects of low sensitivity, high detection limit and serious matrix interference in the prior art, and provide a vitami

Method used

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  • Liquid chromatogram-inductively coupled plasma mass spectrometry detection method of vitamin B12
  • Liquid chromatogram-inductively coupled plasma mass spectrometry detection method of vitamin B12
  • Liquid chromatogram-inductively coupled plasma mass spectrometry detection method of vitamin B12

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Vitamin B 12 The liquid chromatography-inductively coupled plasma mass spectrometry detection method, the steps are as follows:

[0031] (1) Sample processing:

[0032] Sample 1: Juice sample

[0033] Weigh 10g (accurate to 0.01g) of the fruit juice sample into a 25mL brown volumetric flask, and dilute to the mark with water to obtain the liquid to be purified 1;

[0034] Sample Two: Cereal Products

[0035] Weigh 5g of cereal products (accurate to 0.01g) into a 50mL centrifuge tube, add 25mL of 0.01mol / L hydrochloric acid solution, vortex and mix for 1min, sonicate for 15min, centrifuge at 3900r / min for 15min, collect the supernatant in a 50mL brown capacity In the bottle, extract the solid residue once more with 20mL of 0.01mol / L hydrochloric acid solution, combine the extracted supernatant, and dilute to the mark with water to obtain the liquid to be purified 2;

[0036] Sample Three: Milky Beverages

[0037] Weigh 10g (accurate to 0.01g) of milk-containing beve...

Embodiment 2

[0053] Vitamin B 12 The liquid chromatography-inductively coupled plasma mass spectrometry detection method, the steps are as follows:

[0054] (1) Sample processing:

[0055] Using sample one (juice sample) in Example 1, process the obtained liquid 1 to be purified;

[0056] (2) Accurately pipette 10mL of the above-mentioned solution 1 to be purified, pass through the activated solid phase extraction column, use 6mL 5% v / v acetonitrile solution to rinse the interfering substances from the solid phase extraction column, and finally use 4mL 20% v / v acetonitrile solution will vitamin B 12 For elution, collect all the eluate, dilute the eluate according to the content, and pass through a 0.22 μm microporous membrane to obtain the test solution for detection and analysis.

[0057] (3) Accurately pipette vitamin B respectively 12 Standard working solutions of 5 μL, 10 μL, 25 μL, 50 μL, 100 μL, 125 μL, 250 μL, and dilute to 10 mL with pH 5.0 aqueous solution. The concentration...

Embodiment 3

[0065] Vitamin B 12 The liquid chromatography-inductively coupled plasma mass spectrometry detection method, the steps are as follows:

[0066] (1) Sample processing:

[0067] Using sample one (juice sample) in Example 1, process the obtained liquid 1 to be purified;

[0068] (2) Accurately pipette 10mL of the above-mentioned solution 1 to be purified, pass through the activated solid phase extraction column, use 5mL 10% v / v acetonitrile solution to rinse the interfering substances from the solid phase extraction column, and finally use 3.0mL 40% v / v acetonitrile solution to vitamin B 12 For elution, collect all the eluate, dilute the eluate according to the content, pass through a 0.22 μm microporous membrane, and wait for detection and analysis.

[0069] (3) Accurately pipette vitamin B respectively 12 Standard working solutions of 5 μL, 10 μL, 25 μL, 50 μL, 100 μL, 125 μL, 250 μL, and dilute to 10 mL with pH 5.0 aqueous solution. The concentrations are: 0.5ng / mL, 1.0ng...

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Abstract

The invention discloses a liquid chromatogram-inductively coupled plasma mass spectrometry detection method of vitamin B12. According to the liquid chromatogram-inductively coupled plasma mass spectrometry detection method of vitamin B12, high performance liquid chromatography and inductively coupled plasma mass spectrometry are combined, so that the obtained liquid chromatogram-inductively coupled plasma mass spectrometry detection method of vitamin B12 is stable and reliable, is high in anti-matrix interference performance, low in detection limit, simple in operation, and high in speed, and is especially suitable for determination of vitamin 12 in food, and novel reference is provided for detection and analysis of vitamin B12 in food.

Description

technical field [0001] The invention relates to the technical field of component detection, in particular to a vitamin B 12 liquid chromatography-inductively coupled plasma mass spectrometry detection method. Background technique [0002] Vitamin B 12 Also known as cobalamin or cyanocobalamin, it is a B vitamin composed of cobalt-containing porphyrin compounds. Vitamin B 12 The detection of vitamin B is one of the most difficult problems in food analysis in the world. The first is vitamin B in animal food. 12 There are various structural forms such as cyanocobalamin, hydroxocobalamin, adenosylcobalamin and methylcobalamin, and traditional analysis methods are difficult to reflect its actual content; secondly, vitamin B 12 Low content in food, 10 -9 Level, usually the food content is 0.4 ~ 3μg / 100g; in addition, vitamin B 12 Mainly vitamin B 12 -Exist in the form of intrinsic factor complex, it is very difficult to fully extract and separate, and other chromatographic ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 刘杰范志勇张莉彭青枝文红王会霞周陶鸿朱晓玲龚蕾
Owner HUBEI PROVINCIAL INST FOR FOOD SUPERVISION & TEST
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