Platelet antibody detection kit and use method thereof

An antibody detection and platelet technology, applied in the field of blood transfusion testing, can solve the problems of low sensitivity, high technical requirements, poor specificity, etc.

Inactive Publication Date: 2017-07-14
北京乐普诊断科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to overcome the practical problems of other clinical platelet antibody detection methods such as low sensitivity, poor specificity, complicated clinical operation, long detection time, difficulty in interpreting results, and lack of corresponding reagent quality control; the existing microcolumn gel method for platelet antibody detection Indicating e

Method used

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  • Platelet antibody detection kit and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Such as figure 1 As shown, the platelet antibody detection kit includes a detection plate (1), a gel tube (2) and a parafilm (3), and there are six gel tubes (2), which are arranged on the detection plate ( 1), the parafilm (3) is arranged above the detection plate (1) and covers the tops of the six gel tubes (2), each of the gel tubes (2) from top to bottom Each includes a nozzle (2.1), a buffer chamber (2.2) and a reaction chamber (2.3), the opening of the nozzle (2.1) faces upward, and the outer wall of each gel tube (2) is also provided with two supports plate (2.4), the two support plates (2.4) are respectively arranged at the front and back of each gel tube (2), and the detection plate (1) is a polypropylene plastic transparent plate. The six gel tubes (2) contain anti-human globulin reagent IgG with a titer of 64-128, 0.1-1M imidazole buffer, 1%-10% bovine serum albumin, 0.5%- 1% sodium chloride, 0.1%-1% sodium azide, 0.1%-2% Tween-20; contain 2 / 3 volume of G-2...

Embodiment 2

[0065]The method for platelet antibody detection using the platelet antibody detection kit of Example 1, the method includes the following steps: adding a positive control tube containing anti-human platelet antibody, platelet to the positive control tube of the platelet antibody detection kit Indicating erythrocytes and platelet antigens for positive reactions; adding negative control not containing anti-human platelet antibodies, platelets indicating erythrocytes and platelet antigens to negative control tubes for negative reactions; adding platelets indicating erythrocytes, platelet antigens and Serum samples were reacted.

[0066] Among them: specifically include the following operations:

[0067] S1. A method for preparing indicator erythrocytes coated with mouse anti-human platelet monoclonal antibody, and placing the indicator erythrocytes in vials after subpackaging;

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Abstract

The invention provides a platelet antibody detection kit and a use method thereof. The platelet antibody detection kit comprises a detection plate (1), six gel tubes (2) and an opening sealing film (3), wherein the six gel tubes (2) are arranged at the top end of the detection plate (1) in a left and right arranging way; the opening sealing film (3) is arranged above the detection plate (1), and covers the top ends of the six gel tubes (2); each of six gel tubes (2) contains an anti-human globulin reagent and sephadex; the first gel tube and the sixth gel tube are respectively a positive contrast tube and a negative contrast tube, and the second gel tube, the third gel tube and the fourth gel tube are reaction tubes. The platelet antibody detection kit has the advantages that anti-human IgG and IgG sensitized erythrocyte are used as indication systems to replace anti-human IgG-coated aldehydated erythrocyte, the detection result is clear and accurate, and the repeatability is strong; the effect that detection result can be accurately judged by the naked eye and automatic robot at the same time, and the detection result is easily standardized.

Description

technical field [0001] The invention belongs to the technical field of blood transfusion testing, and relates to a platelet antibody detection kit and a use method thereof. Background technique [0002] With the significant increase of patients with tumors and blood system diseases and the rapid development of clinical transfusion medicine, the amount of platelet transfusion is increasing year by year. Platelet transfusion is an effective measure to increase platelet count, prevent bleeding, and protect patients' life safety. Its safe, scientific and effective transfusion has become an inevitable requirement for the development of clinical blood transfusion technology. However, at present, most of the platelet transfusions in my country still use random transfusion (that is, "blind transfusion"). Improper platelets are quickly destroyed and cleared by the body's immune system after transfusion, resulting in frequent occurrence of platelet ineffectiveness, which not only wast...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 魏明明陈江莎杨洋王占伟邱笑违
Owner 北京乐普诊断科技股份有限公司
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