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A kind of method of tissue culture and rapid propagation of Sorrel sativa

A technique for cultivating sagebrush and tissue culture, which is applied in the fields of plant tissue culture and ecological environment restoration, can solve problems such as pollution and provenance degradation, and achieves the effects of easy operation, convenient operation and high yield.

Active Publication Date: 2019-05-28
SHUISHENGZAOAN BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is a pity that there is still a lack of mature technology at present, which can multiply the high-quality engineered seedlings of S. grateifolia on a large scale, and at the same time overcome the problem of degraded provenance and serious pollution of this species in the natural environment.

Method used

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  • A kind of method of tissue culture and rapid propagation of Sorrel sativa
  • A kind of method of tissue culture and rapid propagation of Sorrel sativa
  • A kind of method of tissue culture and rapid propagation of Sorrel sativa

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Experimental program
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Effect test

Embodiment 1

[0044] A, explant selection and pretreatment: choose bright green color, grow vigorously and can germinate the stem section of axillary buds as explants, soak with alkaline detergent for 10min, then rinse with tap water for 1 hour, and rinse with tap water for 1 hour. Repeatedly rinse with distilled water for 4-5 times, then wash with sterile water for 4-5 times, and place on a sterile operating table;

[0045] B. Preparation of sterile explants: Soak in 75% ethanol for 15 seconds on a sterile operating table, rinse with sterile water for 3-4 times, and then use 0.1% mercuric chloride (add a few drops of Tween 80) Disinfect the surface for 5 minutes, rinse with sterile water 4-5 times, blot the water dry on sterile filter paper, and set aside;

[0046] C. Cluster bud induction: select MS solid medium with 0.10 mg / L KT and 0.01mg / L NAA, the mass concentration of sucrose is 3%, the pH is adjusted to 5.8-6.0, and the solid medium is packed in a tissue culture bottle; Sterilize a...

Embodiment 2

[0054] A, selection and pretreatment of explants: select the bright green color, grow vigorously and can germinate the axillary buds of the stem section of S. argentinae as explants, soak with alkaline detergent for 10min, then rinse with tap water for 1 hour, and rinse with tap water for 1 hour. Repeatedly rinse with distilled water for 4-5 times, then wash with sterile water for 4-5 times, and place on a sterile operating table;

[0055] B. Preparation of sterile explants: Soak in 75% ethanol for 15 seconds on a sterile operating table, rinse with sterile water for 3-4 times, and then use 0.1% mercuric chloride (add a few drops of Tween 80) Disinfect the surface for 5 minutes, rinse with sterile water 4-5 times, blot the water dry on sterile filter paper, and set aside;

[0056] C. Cluster bud induction: select 0.10 mg / L KT and 0.01 mg / L NAA MS solid medium, the mass concentration of sucrose is 3%, the pH is adjusted to 5.8-6.0, and the solid medium is packed in a tissue cul...

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Abstract

The invention discloses a tissue culture and rapid propagation method for stuckenia pectinata. The method comprises the following steps: A) selecting and pre-treating explants: selecting robust and green plant stems as explants; B) preparing sterile explants: treating the explants with ethyl alcohol and mercuric chloride to prepare the sterile explants; C) inducing multiple shoots: confirming suitable hormone variety and concentration ratio conditions and inducing the bud growth; D) performing multiplication culture: adjusting hormone ratio for performing multiplication culture; E) performing rooting culture: selecting hormone variety and concentration ratio for performing rooting culture; F) performing enlarging cultivation: selecting sterile seedlings in suitable length and placing into a proliferation medium, performing enlarging subculture and industrially producing the sterile plants; G) hardening seedlings and transplanting: opening a bottle cover in an incubator and culturing for 2 days, transferring into natural water and culturing. The method disclosed by the invention is not limited by season environment, the sterile plants are cultured and the continuous seedlings are supplied for the recovery of aquatic ecosystem.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and specifically relates to a method for tissue culture and rapid propagation of the submerged plant sarcophagus. The method adopts complete aseptic and strict temperature control measures to realize the submerged plant sarcophagus Vegetables are produced in large quantities in the laboratory and can be extended to the field of ecological environment restoration. Background technique [0002] Selina genus is a submerged plant distributed all over the world. Ginger eye vegetable grows in various water bodies such as river ditches, canals, ponds, etc. The water bodies are mostly slightly acidic or neutral, and it is also found in a few slightly alkaline water bodies and salt water in Northwest China. Globally distributed, especially common in the temperate waters of the two hemispheres, it has an outstanding purification effect on nitrogen and phosphorus in water. [0003] In recent ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 不公告发明人
Owner SHUISHENGZAOAN BIOTECH WUHAN CO LTD