Method for preparing waxberry extract for cigarette by immobilized saccharomycetes

A technology of yeast and extract, which is applied in the field of preparation of bayberry extract for tobacco, to achieve the effects of strong anti-toxicity, increased fruity sweet aroma, and reduced irritation

Inactive Publication Date: 2017-07-18
WUHAN HUANGHELOU FLAVOR & SPICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Method for preparing waxberry extract for cigarette by immobilized saccharomycetes

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Experimental program
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Effect test

Embodiment 1

[0020] (1) Take fresh bayberry, remove the core, squeeze the juice, sterilize at 121°C for 20 minutes, cool, and filter to obtain bayberry juice;

[0021] (2) Saccharomyces bacterium suspension preparation: inoculate saccharomycete in broth culture medium, the specific formula of broth culture medium is (per liter): peptone 10g, beef extract powder 3g, sodium chloride 5g, final pH7. 3±0.1, cultured for 48 hours at a temperature of 37°C and a stirring speed of 50 rpm, took the culture solution and centrifuged at 8000 rpm, and the wet cells after centrifugation were used for later use;

[0022] (3) Sodium alginate-CaCl of yeast 2 Immobilization: Prepare a sodium alginate solution with a mass fraction of 4%, and then add 3% of its volume of the yeast wet cell prepared in the step (2) to prepare a mixed solution, and use a peristaltic pump to pass through a 1mm aperture Add the prepared mixture dropwise to the CaCl with a mass fraction of 3% 2 solution, into balls, and the CaCl ...

Embodiment 2

[0026] (1) Take fresh bayberry, remove the core, squeeze the juice, sterilize at 121°C for 20 minutes, cool, and filter to obtain bayberry juice;

[0027] (2) Saccharomyces bacterium suspension preparation: inoculate saccharomycete in broth culture medium, the specific formula of broth culture medium is (per liter): peptone 10g, beef extract powder 3g, sodium chloride 5g, final pH7. 3±0.1, cultured for 48 hours at a temperature of 37°C and a stirring speed of 50 rpm, took the culture solution and centrifuged at 8000 rpm, and the wet cells after centrifugation were used for later use;

[0028] (3) Sodium alginate-CaCl of yeast 2 Immobilization: Prepare a sodium alginate solution with a mass fraction of 5%, then add 4% of its volume of the yeast wet cell prepared in the step (2), prepare a mixed solution, and use a peristaltic pump to pass through a 3mm aperture Add the prepared mixture dropwise to the CaCl with a mass fraction of 4% 2 solution, into balls, and the CaCl 2 The...

Embodiment 3

[0032] (1) Take fresh bayberry, remove the core, squeeze the juice, sterilize at 121°C for 20 minutes, cool, and filter to obtain bayberry juice;

[0033] (2) Saccharomyces bacterium suspension preparation: inoculate saccharomycete in broth culture medium, the specific formula of broth culture medium is (per liter): peptone 10g, beef extract powder 3g, sodium chloride 5g, final pH7. 3±0.1, cultured for 48 hours at a temperature of 37°C and a stirring speed of 50 rpm, took the culture solution and centrifuged at 8000 rpm, and the wet cells after centrifugation were used for later use;

[0034] (3) Sodium alginate-CaCl of yeast 2 Immobilization: Prepare a sodium alginate solution with a mass fraction of 5%, then add 5% of its volume of the yeast wet cell prepared in the step (2), prepare a mixed solution, and use a peristaltic pump to pass through a 4mm aperture Add the prepared mixed solution drop by drop to the CaCl with a mass fraction of 6%. 2solution, into balls, and the ...

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Abstract

The invention provides a method for preparing a waxberry extract for cigarette by immobilized saccharomycetes. The method comprises the following steps: (1) juicing fresh waxberry; (2) preparing a saccharomycetes bacterial suspension; (3) immobilizing saccharomycetes with sodium alginate-CaCl2; (4) performing fermenting reaction on the immobilized saccharomycetes and the waxberry juice; (5) filtering fermentation liquor, thereby acquiring clear fermentation liquor, and then performing alcohol precipitation and vacuum concentration, thereby acquiring the waxberry extract. According to the invention, the waxberry is taken as a raw material and the immobilized saccharomycetes are utilized to ferment the waxberry juice, so that the high-quality waxberry extract for cigarette can be acquired. The extract in cigarette has the functions of enriching cigarette flavor, inducing cigarette fragrance, increasing fruity sweet and fragrance, enhancing smoke saliva-generating feel, softening and fining smoke, relieving throat irritation, and the like.

Description

technical field [0001] The invention relates to the field of tobacco additives, in particular to a preparation method of using immobilized yeast to prepare tobacco bayberry extract. Background technique [0002] Flavoring is a key link in the cigarette process, and it is one of the effective means to improve the determinant of product style and improve the quality of cigarette aroma. At present, flavors and fragrances for tobacco are mainly prepared through extraction and chemical synthesis of natural animals and plants. Due to limited natural plant resources and harsh conditions for chemical synthesis. With the development of biotechnology, in recent years, the use of microbial fermentation to prepare tobacco flavor raw materials provides an economical and efficient way for the production of tobacco flavor raw materials, and develops some new types of tobacco flavor raw materials that cannot be produced by traditional methods. [0003] Microbial immobilization technology ...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12N11/10C11B9/00C12R1/865
CPCC11B9/00C12N11/10C12P1/02C12N1/185C12R2001/865
Inventor 庞登红尹团章王珊张磊苏宏
Owner WUHAN HUANGHELOU FLAVOR & SPICES
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